Relationship among obesity, caries and gut/oral microbiome in preschoolers aging 3 to 5 years in Bragança Paulista-SP

• Grant number: 15/24600-2
• Support Opportunities: Regular Research Grants
• Duration: April 01, 2016 - December 31, 2018
• Field of knowledge: Health Sciences - Collective Health - Preventive Medicine
• Principal Investigator: Thaís Parisotto Ulmer
• Grantee: Thaís Parisotto Ulmer
• Host Institution: Universidade São Francisco (USF). Campus Bragança Paulista. Bragança Paulista , SP, Brazil

Abstract

Controlling worldwide diseases like obesity and early childhood caries is one of the most important public health challenges nowadays. Therefore, studies identifying risk indicators related to these diseases should be encouraged. The aim of this research is to evaluate the relationship between obesity, early childhood caries, frequency of sugar exposure, immunological factors and oral/gut microbiome. Initially, 400 children, aging 3-5 years, who attend public preschools in Bragança-SP will be submitted to clinical examinations for nutritional status diagnosis using the body mass index according to World Health Organization (WHO) standards. Considering that the obesity prevalence in this population is about 10%, two groups will be formed: 1. Obese (n=40) 2. Eutrofiphic (n=40). Additional anthropometric measurements (waist-hip circumference and triceps skinfold will be also measured) and caries índex will be assessed using WHO criteria, modified by the inclusion of active white spot lesions. The dental biofilm of all free smooth surfaces and fecal samples will be collected for evaluation of Streptococcus mutans/sobrinus (mutans streptococci), Lactobacillus and the proportion of Firmicutes x Bacteroidetes bacteria phylum by real-time PCR. Saliva will be collected without stimulation in order to evaluate Interleukin-6 (IL-6), tumor necrosis factor a (TNF-a), total secretory immunoglobulin A (IGA) by ELISA test and to evaluate IgA specific for glucan binding protein B (virulence antigen of Streptococcus mutans, the main pathogen of caries) a flow cytometry will be used. The frequency of sugar exposure will be assessed by a diet chart. The data will be analyzed by logistic regression, in order to build a model to identify among the studied factors, which are more relevant for obesity/caries (± = 0.05). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RIZZARDI, KARINA FERREIRA; INDIANI, CLAUDIA MARIA DOS SANTOS PEREIRA; MATTOS-GRANER, RENATA DE OLIVEIRA; DE SOUSA, EMERSON TAVARES; NOBRE-DOS-SANTOS, MARINES; PARISOTTO, THAIS MANZANO. Firmicutes Levels in the Mouth Reflect the Gut Condition With Respect to Obesity and Early Childhood Caries. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 11, MAY 27 2021. (15/24600-2)

DOS SANTOS PEREIRA INDIANI, CLAUDIA MARIA; RIZZARDI, KARINA FERREIRA; CRESCENTE, CAMILA LOPES; STEINER-OLIVEIRA, CAROLINA; NOBRE-DOS-SANTOS, MARINES; PARISOTTO, THAIS MANZANO. Relationship Between Mutans Streptococci and Lactobacilli in the Oral Cavity and Intestine of Obese and Eutrophic Children With Early Childhood Caries-Preliminary Findings of a Cross-Sectional Study. FRONTIERS IN PEDIATRICS, v. 8, DEC 11 2020. (15/24600-2)

LEME, LUCIA APARECIDA FEDERIGHI PEREIRA; RIZZARDI, KARINA FERREIRA; SANTOS, ISIS BOLSONARO; PARISOTTO, THAIS MANZANO. Exploring the Relationship between Salivary Levels of TNF-alpha, Lactobacillus acidophilus, Lactobacillus gasseri, Obesity, and Caries in Early Childhood. PATHOGENS, v. 11, n. 5, p. 11-pg., 2022-05-01. (15/24600-2)