Improving antimicrobial photodynamic therapy for infectious disease associating peptide aurein 1.2

• Grant number: 16/05345-4
• Support Opportunities: Regular Research Grants
• Duration: July 01, 2016 - June 30, 2018
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Carla Raquel Fontana Mendonça
• Grantee: Carla Raquel Fontana Mendonça
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

According to the World Health Organization (WHO), infections by resistant bacteria affect more than 2 million people, causing more than 20,000 deaths per year in the United States and Europe. Bacterial resistance has grown over the years, however, new antibiotics development rate has declined over the past three decades, with only one new breakthrough therapeutic class since the 1980s. This growing antibiotic resistance managed to search by drugs and / or therapies that are not only capable of eliminating the resistant microorganisms, but also have characteristics which hinder the development of resistance. In this context, Photodynamic Therapy (PDT) and antimicrobial peptides are highlighted, being identified as the most promising alternatives to antimicrobial drugs for the treatment of localized infections. In this study, methylene blue and curcumin will be used as photosensitizers and 1.2 aureína peptide, a peptide of 13 amino acid residues will also be tested. Both therapeutic approaches will be assessed to increase bacterial reduction, both individually and in combination. Still, new drug delivery systems will be developed and evaluated with the aim of incorporating the system in the same photosensitizer and the peptide in order to optimize the combination therapy. Thus, this project aims to develop new drugs and pharmaceutical formulations that optimize the effect of photodynamic therapy on bacterial reduction. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VELASQUEZ, ANGELA M. A.; BARTLETT, PAULA J.; LINARES, IRWIN A. P.; PASSALACQUA, THAIS G.; TEODORO, DAPHNE D. L.; IMAMURA, KELY B.; VIRGILIO, STELA; TOSI, LUIZ R. O.; LEITE, ALINE DE LIMA; BUZALAF, MARILIA A. R.; et al. ew Insights into the Mechanism of Action of the Cyclopalladated Complex (CP2) in Leishmania: Calcium Dysregulation, Mitochondrial Dysfunction, and Cell Deat. Antimicrobial Agents and Chemotherapy, v. 66, n. 1, JAN 2022. (16/18191-5, 16/05345-4, 18/23015-7, 20/04415-4, 17/03552-5, 16/19289-9, 19/21661-1)

FRADE, MARIA LUCIA; DE ANNUNZIO, SARAH RAQUEL; FIORAMONTI CALIXTO, GIOVANA MARIA; VICTORELLI, FRANCESCA DAMIANI; CHORILLI, MARLUS; FONTANA, CARLA RAQUEL. Assessment of Chitosan-Based Hydrogel and Photodynamic Inactivation against Propionibacterium acnes. Molecules, v. 23, n. 2, FEB 2018. (16/05345-4)

LOPERA SEPULVEDA, ALEX ARBEY; ARENAS VELASQUEZ, ANGELA MARIA; PATINO LINARES, IRWIN ALEXANDER; DE ALMEIDA, LETICIA; FONTANA, CARLA RAQUEL; GARCIA, CLAUDIA; SILVA GRAMINHA, MARCIA APARECIDA. Efficacy of photodynamic therapy using TiO2 nanoparticles doped with Zn and hypericin in the treatment of cutaneous Leishmaniasis caused by Leishmania amazonensis. Photodiagnosis and Photodynamic Therapy, v. 30, JUN 2020. (16/05345-4, 17/03552-5, 16/19289-9, 18/23015-7)

LOPERA, A. A.; VELASQUEZ, A. M. A.; CLEMENTINO, L. C.; ROBLEDO, S.; MONTOYA, A.; DE FREITAS, L. M.; BEZZON, V. D. N.; FONTANA, C. R.; GARCIA, C.; GRAMINHA, M. A. S.. Solution-combustion synthesis of doped TiO2 compounds and its potential antileishmanial activity mediated by photodynamic therapy. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 183, p. 64-74, JUN 2018. (17/03552-5, 16/05345-4, 16/19289-9, 14/24581-5, 13/08248-1)

FONTANA, CARLA RAQUEL; GRECCO, CLOVIS; BAGNATO, VANDERLEI SALVADOR; DE FREITAS, LAURA MARISE; BOUSSIOS, CONSTANTINOS I.; SOUKOS, NIKOLAOS S.. Molecular analyses of two bacterial sampling methods in ligature-induced periodontitis in rats. CLINICAL AND EXPERIMENTAL DENTAL RESEARCH, v. 4, n. 1, p. 19-24, FEB 2018. (16/05345-4, 13/07276-1)

FOGACA DE AGUIAR COLETTI, TATIANA MARIA STARCK; DE FREITAS, LAURA MARISE; FUSCO ALMEIDA, ANA MARISA; FONTANA, CARLA RAQUEL. Optimization of Antimicrobial Photodynamic Therapy in Biofilms by Inhibiting Efflux Pump. Photomedicine and Laser Surgery, v. 35, n. 7, p. 378-385, JUL 2017. (16/05345-4)

ESTEVAM-ALVES, REGINA; DIAS FERREIRA, PAULO HENRIQUE; COATRINI, ANDREY C.; OLIVEIRA, JR., OSVALDO N.; FONTANA, CARLA RAQUEL; MENDONCA, CLEBER RENATO. Femtosecond Laser Patterning of the Biopolymer Chitosan for Biofilm Formation. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 17, n. 8, AUG 2016. (11/12399-0, 16/05345-4, 13/14262-7)

DE SOUSA, JULIANA FERREIRA; SERAFIM, RODOLFO BORTOLOZO; DE FREITAS, LAURA MARISE; FONTANA, CARLA RAQUEL; VALENTE, VALERIA. DNA repair genes in astrocytoma tumorigenesis, progression and therapy resistance. GENETICS AND MOLECULAR BIOLOGY, v. 43, n. 1, 1, 2020. (16/05345-4, 13/13465-1, 14/24581-5, 18/05018-9, 13/08135-2)

JULIANA FERREIRA DE SOUSA; RODOLFO BORTOLOZO SERAFIM; LAURA MARISE DE FREITAS; CARLA RAQUEL FONTANA; VALERIA VALENTE. DNA repair genes in astrocytoma tumorigenesis, progression and therapy resistance. GENETICS AND MOLECULAR BIOLOGY, v. 43, n. 1, 2019-12-13. (14/24581-5, 18/05018-9, 13/13465-1, 13/08135-2, 16/05345-4)

DE FREITAS, LAURA MARISE; SERAFIM, RODOLFO BORTOLOZO; DE SOUSA, JULIANA FERREIRA; MOREIRA, THAIS FERNANDA; DOS SANTOS, CLAUDIA TAVARES; BAVIERA, AMANDA MARTINS; VALENTE, VALERIA; SOARES, CHRISTIANE PIENNA; FONTANA, CARLA RAQUEL. Photodynamic therapy combined to cisplatin potentiates cell death responses of cervical cancer cells. BMC CANCER, v. 17, FEB 10 2017. (16/05345-4, 12/25414-0)

DE ANNUNZIO, SARAH RAQUEL; DE FREITAS, LAURA MARISE; BLANCO, ANA LIGIA; DA COSTA, MARDOQUEU MARTINS; CARMONA-VARGAS, CHRISTIAN C.; DE OLIVEIRA, KLEBER THIAGO; FONTANA, CARLA RAQUEL. Susceptibility of Enterococcus faecalis and Propionibacterium acnes to antimicrobial photodynamic therapy. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 178, p. 545-550, JAN 2018. (16/05345-4, 14/24581-5, 15/21110-4)