Influence of the citrotherapy in the production of prostaglandins by healthy women during menstrual period

• Grant number: 15/23679-4
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2016
• End date: May 31, 2018
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Priscila Maria de Andrade Borra
• Grantee: Priscila Maria de Andrade Borra
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated researchers: Maria Fátima das Graças Fernandes da Silva ; Maristela Carbol ; Ricardo Carneiro Borra

Abstract

The woman's body suffers several hormonal changes for the preparation of pregnancy. Thus, estrogen and progesterone act in response to fertilization, or not. If there is no implantation, hormonal levels decrease, increasing the concentration of prostaglandin (PG) through action of cyclooxygenase enzyme. The PGF2± and PGE2, heavily produced during menstruation, are responsible for part of the endometrial contraction, causing severe uterine pain, known as dysmenorrhea. Depending on the intensity, cramps may be controlled by the use of anti-inflammatory drugs, blocking prostaglandin synthesis. However, this kind of therapeutic option produces side effects. In this sense, the use of phytotherapy products has been proposed as an alternative for the control of inflammatory symptoms. The Taiti lemon (Citrus latifolia), has been shown to be effective in controlling the effects of menstruation in women who have deregulated cycles. However, these mechanisms are not entirely known, but the inhibition of the arachidonic acid cascade is one possibility. Thus, it is intended to study the effect of citrus therapy based on Taiti lemon in the production of PGs and gene expression of transcripts IL-1b, IL-6, TNFa, AKR1B1 and AKR1C3 involved in the PGs synthesis or on uterine inflammatory process in healthy women during menstruation. For this, fertile period women in the age group of 18-40 years will be invited and divided into three groups, depending on the stimulation that will be exhibited: Lemon Group (G1, n = 15), Meloxicam Group (G2, n = 15) and control group (G3, n = 15). The prostaglandin production will be evaluated in the three groups, and the total blood and collected menstrual blood will be used for plasma dosage and cell culture of total blood stimulated or not with LPS to compare lemon inhibitory activity with meloxicam medicine in the genes expression or production of PGE2 and PGF2a and gene expression of transcript involved in the synthesis of the PGs or on uterine inflammatory process. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROBELDO, THAIANE; CANZI, EDIONE FATIMA; DE ANDRADE, PRISCILA MARIA; PEDOTTE SANTANA, JHONNE PEDRO; TEIXEIRA, FELIPE ROBERTI; SPAGNOL, VALENTINE; NORONHA SALES MAIA, BEATRIZ HELENA LAMEIRO; CARBOL, MARISTELA; CANEIRA, ERIKA GONCALVES; DAS GRACAS FERNANDES DA SILVA, MARIA FATIMA; et al. Effect of Tahiti lime (Citrus latifolia) juice on the Production of the PGF2 alpha/PGE2 and Pro-Inflammatory Cytokines involved in Menstruation. SCIENTIFIC REPORTS, v. 10, n. 1, APR 27 2020. (15/23679-4, 15/23509-1)