Investigation of quinolone resistance mechanisms in environmental bacterial isolates

• Grant number: 15/18990-2
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2016
• End date: December 31, 2018
• Field of knowledge: Biological Sciences - Microbiology
• Principal Investigator: Eliana Guedes Stehling
• Grantee: Eliana Guedes Stehling
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Fluoroquinolones are antimicrobials often prescribed in Brazil and in other countries and used to treat several types of infections, especially those of urinary and gastrointestinal tract. The action of these compounds is due to inhibition of DNA gyrase and topoisomerase IV, preventing replication of bacterial DNA. Mutations in the QRDR region (Quinolone Resistance-Determining Regions) of these genes, especially in the gyrA gene, corresponds to the main quinolone resistance mechanism, however, other mechanisms of resistance may be present, including the acquisition of plasmid determinants [qnr, qepA and aac (6 ') - Ib-cr] and changes in permeability of the efflux pump AcrAB-TolC.The objective of this project is to identify the main resistance mechanisms to quinolones in soil bacterial isolates and identify the main genera from this microbiota that carry such determinants of resistance. (AU)

Scientific publications

(23)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

JOÃO PEDRO RUEDA FURLAN; ANDRÉ PITONDO-SILVA; ELIANA GUEDES STEHLING. Detection of blaNDM-1 in Stenotrophomonas maltophilia isolated from Brazilian soil. Memórias do Instituto Oswaldo Cruz, v. 113, n. 6, 2018-05-14. (15/18990-2)

RUEDA FURLAN, JOAO PEDRO; BRAZ, VANIA SANTOS; RIZZATO PASCHOAL, JONAS AUGUSTO; STEHLING, ELIANA GUEDES. Buttiauxella chrysanthemi sp nov., isolated from a chrysanthemum plantation in Brazil. Archives of Microbiology, v. 200, n. 9, p. 1365-1369, NOV 2018. (15/18990-2)

RUEDA FURLAN, JOAO PEDRO; SANCHEZ, DANILO GARCIA; LAGE GALLO, INARA FERNANDA; STEHLING, ELIANA GUEDES. Replicon typing of plasmids in environmental Achromobacter sp producing quinolone-resistant determinants. APMIS, v. 126, n. 11, p. 864-869, NOV 2018. (15/18990-2)

RUEDA FURLAN, JOAO PEDRO; PITONDO-SILVA, ANDRE; STEHLING, ELIANA GUEDES. Detection of bla(NDM-1) in Stenotrophomonas maltophilia Isolated from Brazilian soil. Memórias do Instituto Oswaldo Cruz, v. 113, n. 6, 2018. (15/18990-2)

BRAZ, VANIA SANTOS; SILVA MORETTO, JESSICA APARECIDA; TONELLI FERNANDES, ANA FLAVIA; STEHLING, ELIANA GUEDES. Change in the antimicrobial resistance profile of Pseudomonas aeruginosa from soil after exposure to herbicides. JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMIN, v. 54, n. 4, p. 290-293, APR 3 2019. (14/02806-5, 15/18990-2)

RUEDA FURLAN, JOAO PEDRO; STEHLING, ELIANA GUEDES. High-level of resistance to beta-lactam and presence of beta-lactamases encoding genes in Ochrobactrum sp and Achromobacter sp isolated from soil. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 11, p. 133-137, DEC 2017. (15/18990-2)

RODRIGUEZ BARDAJI, DANAE KALA; RUEDA FURLAN, JOAO PEDRO; STEHLING, ELIANA GUEDES. Isolation of a polyethylene degrading Paenibacillus sp. from a landfill in Brazil. Archives of Microbiology, v. 201, n. 5, p. 699-704, JUL 2019. (15/18990-2)

SILVA MORETTO, JESSICA APARECIDA; BRAZ, VANIA SANTOS; RUEDA FURLAN, JOAO PEDRO; STEHLING, ELIANA GUEDES. Plasmids associated with heavy metal resistance and herbicide degradation potential in bacterial isolates obtained from two Brazilian regions. ENVIRONMENTAL MONITORING AND ASSESSMENT, v. 191, n. 5, MAY 2019. (15/18990-2)

SILVA MORETTO, JESSICA APARECIDA; RUEDA FURLAN, JOAO PEDRO; TONELLI FERNANDES, ANA FLAVIA; BAUERMEISTER, ANELIZE; LOPES, NORBERTO PEPORINE; STEHLING, ELIANA GUEDES. Alternative biodegradation pathway of the herbicide diuron. INTERNATIONAL BIODETERIORATION & BIODEGRADATION, v. 143, SEP 2019. (15/18990-2)

RUEDA FURLAN, JOAO PEDRO; STEHLING, ELIANA GUEDES. Detection of bla (PER) on an IncA/C Plasmid in Stenotrophomonas maltophilia Isolated from Brazilian Soil. WATER AIR AND SOIL POLLUTION, v. 229, n. 5, MAY 2018. (15/18990-2)

SANCHEZ, DANILO GARCIA; DE MELO, FERNANDA MACIEL; SAVAZZI, EDUARDO ANGELINO; STEHLING, ELIANA GUEDES. Detection of different beta-lactamases encoding genes, including bla (NDM), and plasmid-mediated quinolone resistance genes in different water sources from Brazil. ENVIRONMENTAL MONITORING AND ASSESSMENT, v. 190, n. 7, JUL 2018. (15/18990-2)

RUEDA FURLAN, JOAO PEDRO; STEHLING, ELIANA GUEDES. Draft genome sequence of a multidrug-resistant tetA/IncF-harbouring Escherichia coli ST906 obtained from a soil cultivated with jaboticaba (Plinia cauliflora). JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 16, p. 2-pg., 2019-03-01. (15/18990-2)

RUEDA FURLAN, JOAO PEDRO; SANCHEZ, DANILO GARCIA; FACHIN, ANA LUCIA; STEHLING, ELIANA GUEDES. Presence of -Lactamase Encoding Genes in Burkholderia cepacia Complex Isolated from Soil. MICROBIAL DRUG RESISTANCE, v. 24, n. 4, p. 347-352, MAY 2018. (15/18990-2)

RUEDA FURLAN, JOAO PEDRO; STEHLING, ELIANA GUEDES; PITONDO-SILVA, ANDRE. Importance of Sequencing To Determine Functional bla(TEM) Variants. Antimicrobial Agents and Chemotherapy, v. 61, n. 5, MAY 2017. (15/18990-2, 13/22581-5)

RUEDA FURLAN, JOAO PEDRO; STEHLING, ELIANA GUEDES. Presence of beta-Lactamases Encoding Genes in Soil Samples from Different Origins. WATER AIR AND SOIL POLLUTION, v. 228, n. 4, APR 2017. (15/18990-2)

BRAZ, VANIA SANTOS; RUEDA FURLAN, JOAO PEDRO; PASSAGLIA, JAQUELINE; FALCAO, JULIANA PFRIMER; STEHLING, ELIANA GUEDES. Genotypic diversity and presence of beta-lactamase encoding genes in Pseudomonas aeruginosa isolated from Brazilian soils. APPLIED SOIL ECOLOGY, v. 129, p. 94-97, AUG 2018. (15/18990-2, 16/24716-3)

LAGE GALLO, INARA FERNANDA; RUEDA FURLAN, JOAO PEDRO; SANCHEZ, DANILO GARCIA; STEHLING, ELIANA GUEDES. Heavy metal resistance genes and plasmid-mediated quinolone resistance genes in Arthrobacter sp. isolated from Brazilian soils. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, v. 112, n. 10, p. 1553-1558, OCT 2019. (15/18990-2)

RUEDA FURLAN, JOAO PEDRO; SANCHEZ, DANILO GARCIA; LAGE GALLO, INARA FERNANDA; STEHLING, ELIANA GUEDES. Characterization of Acquired Antimicrobial Resistance Genes in Environmental Stenotrophomonas maltophilia Isolates from Brazil. MICROBIAL DRUG RESISTANCE, v. 25, n. 4, NOV 2018. (15/18990-2)

TONELLI FERNANDES, ANA FLAVIA; WANG, PING; STALEY, CHRISTOPHER; SILVA MORETTO, JESSICA APARECIDA; ALTARUGIO, LUCAS MIGUEL; CAMPANHARO, SARAH CHAGAS; STEHLING, ELIANA GUEDES; SADOWSKY, MICHAEL JAY. Impact of Atrazine Exposure on the Microbial Community Structure in a Brazilian Tropical Latosol Soil. MICROBES AND ENVIRONMENTS, v. 35, n. 2, 2020. (15/18990-2, 17/03982-0)

FURLAN, J. P. R.; PITONDO-SILVA, A.; STEHLING, E. G.. New STs in multidrug-resistant Acinetobacter baumannii harbouring -lactamases encoding genes isolated from Brazilian soils. Journal of Applied Microbiology, v. 125, n. 2, p. 506-512, AUG 2018. (15/18990-2)

RUEDA FURLAN, JOAO PEDRO; STEHLING, ELIANA GUEDES. Detection of beta-lactamase encoding genes in feces, soil and water from a Brazilian pig farm. ENVIRONMENTAL MONITORING AND ASSESSMENT, v. 190, n. 2, FEB 2018. (15/18990-2)

TONELLI FERNANDES, ANA FLAVIA; BRAZ, VANIA SANTOS; BAUERMEISTER, ANELIZE; RIZZATO PASCHOAL, JONAS AUGUSTO; LOPES, NORBERTO PEPORINE; STEHLING, ELIANA GUEDES. Degradation of atrazine by Pseudomonas sp and Achromobacter sp isolated from Brazilian agricultural soil. INTERNATIONAL BIODETERIORATION & BIODEGRADATION, v. 130, p. 17-22, MAY 2018. (15/18990-2)

RUEDA FURLAN, JOAO PEDRO; PITONDO-SILVA, ANDRE; BRAZ, VANIA SANTOS; LAGE GALLO, INARA FERNANDA; STEHLING, ELIANA GUEDES. Evaluation of different molecular and phenotypic methods for identification of environmental Burkholderia cepacia complex. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, v. 35, n. 3, MAR 2019. (15/18990-2)