BRAIN INFLAMMATION IN DOGS WITH VISCERAL LEISHMANIASIS: Activation of Innate and Adaptative Immune Response.

• Grant number: 16/02384-9
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2016
• End date: May 31, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Gisele Fabrino Machado
• Grantee: Gisele Fabrino Machado
• Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Associated researchers: Flavia Lombardi Lopes ; Guilherme Dias de Melo ; Paulo Ricardo Dell'Armelina Rocha

Abstract

Visceral leishmaniasis (VL) is a disease with different clinical manifestations in dogs, varying fromsubclinical to generalized. Dogs chronically infected may present neurologic deficits, however only fewreports have characterize lesions that may contribute to better understand the pathogenesis of theneurologic form of VL. Results from previous projects have demonstrated the presence of inflammatorylesions in the brain of dogs, characterized by meningitis and choroiditis, composed by T lymphocytes,glial cells activation, neuronal degeneration, as well as the presence of matrix metalloproteinases andexpression of inflammatory mediators. These data indicate that during VL there are stimuli to establishthe inflammatory alterations, even without the detection of the protozoa in the brain. The pathogenesis ofthese lesions was not fully understand. The usefulness of PCR Profiler technology allows to simultaneously evaluate 84 of genes expressed of different ways, allowing the selection of genes for future studies. Thus, the first aim of this study is to characterize the genic expression in the brain (eg.periventricular region) of dogs with and without visceral leishmaniasis. We hope to continue previousprojects, evaluating brain lesions in dogs VL and investigating the mechanisms and genes related toinflammation and morphological changes in the brain of the dogs with VL comparing both immunological responses in the spleen and in the brain (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GRANO, FERNANDA G.; SILVA, JOSE EDUARDO DOS S.; MELO, GUILHERME D.; DE SOUZA, MILENA S.; LIMA, VALERIA M. F.; MACHADO, GISELE F.. Toll-like receptors and cytokines in the brain and in spleen of dogs with visceral leishmaniosis. Veterinary Parasitology, v. 253, p. 30-38, APR 15 2018. (13/25498-1, 16/02384-9)

SILVA, JOSE EDUARDO DOS SANTOS; JUSSIANI, GIULIA GONCALVES; GRANO, FERNANDA GRECCO; PELISSARI, MARIA CECILIA CLARINDO; DE MELO, GUILHERME DIAS; WATANABE, TATIANE TERUMI NEGRAO; DE LIMA, VALERIA FELIX; MACHADO, GISELE FABRINO. Increased CCL-5 (RANTES) Gene Expression in the Choroid Plexus of Dogs with Canine Leishmaniosis. ANIMALS, v. 13, n. 13, p. 14-pg., 2023-07-01. (16/02384-9)

GRANO, FERNANDA GRECCO; DOS SANTOS SILVA, JOSE EDUARDO; DE MELO, GUILHERME DIAS; MACHADO, GISELE FABRINO. Leishmania hide-and-seek: Parasite amastigotes in the choroid plexus of a dog with neurological signs in an endemic municipality in Brazil. VETERINARY PARASITOLOGY- REGIONAL STUDIES AND REPORTS, v. 17, p. 4-pg., 2019-08-01. (16/02384-9, 14/01202-9, 13/25498-1)

JUSSIANI, GIULIA GONCALVES; MARCO, KAREN SANTOS; BERTOLO, PAULO HENRIQUE LEAL; VASCONCELOS, ROSEMERI DE OLIVEIRA; MACHADO, GISELE FABRINO. Thymic changes due to leishmaniasis in dogs: An immunohistochemical study. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, v. 247, p. 9-pg., 2022-03-28. (16/02384-9, 17/26964-7)