Fabrication and characterisation of nanoelectrochemical sensors for the study of single cells and single nanoparticles

• Grant number: 16/50020-6
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2016
• End date: May 31, 2018
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Agreement: University of Southampton
• Mobility Program: SPRINT - Projetos de pesquisa - Mobilidade
• Principal Investigator: Mauro Bertotti
• Grantee: Mauro Bertotti
• Principal researcher abroad: Guy Denuault
• Institution abroad: University of Southampton, England
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 15/20776-9 - Fabrication of nanometric probes for the study of biochemical processes by using Scanning Electrochemical Microscopy, AP.R

Abstract

The primary research objetive of this project is to explore the use of nanoelectrochemical sensors to study single cells and single nanoparticles. The proposed scientific activity will focus on: 1) the application of nanoelectrodes to get information on reactive oxygen/nitrogen species and thioredoxin proteins in macrophages after an inflammatory activation; 2) scanning electrochemical microscopy (SECM) studies to investigate the role of mitochondria on the respiration process; 3) a study of oxygen adsorption on single nanoparticles grown on nanoelectrodes; 4) a study of oxygen reduction on single nanoparticles grown on nanoelectrodes. In addition, the project includes the organization of a two-day international workshop on nanoelectrodes and we will seek the financial support of sponsors. The proposal is led by two investigators with established track records in electroanalytical chemistry, microelectrochemistry and scanning electrochemical microscopy. (AU)