Comparative and integrative analysis of the transcriptome and the microRNAoma in skeletal muscle of Colossoma macropomum (tambaqui), Piaractus mesopotamicus (pacu) and its hybrid, tambacu: molecular, in silico, in vitro and in vivo approaches.

• Grant number: 16/05009-4
• Support type: Regular Research Grants
• Duration: July 01, 2016 - September 30, 2018
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal researcher: Maeli Dal Pai
• Grantee: Maeli Dal Pai
• Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Assoc. researchers: Daniel Garcia De La Serrana Castillo ; Edson Assunção Mareco ; Robson Francisco Carvalho

Abstract

Colossoma macropomum (tambaqui) and Piaractus mesopotamicus (pacu) have wide acceptance in the market, especially because they have desirable characteristics for the production environment. Although these species have similar eating habits, there are wide variations related to adaptation to cultivation temperature and growth rate. Tambaqui has a higher growth rate compared to pacu which has a greater adaptation to lower temperatures. However, considering the tambacu, resulted from the hybridization between tambaqui and pacu, this presents characteristics that overlap those observed in tambaqui and pacu, and therefore, most suitable for the cultivation environment. Despite the importance of pacu, tambaqui and tambacu for Brazilian aquaculture programs, genetic information for these genotypes are extremely scarce in specialized databases. In this sense, we intend to characterize the transcriptome (RNA transcripts) and microRNAome in the white muscle of the three genotypes. After this, an integrative and comparative analysis of the transcriptome and microRNAome of the three genotypes will be accomplished with the following specific objectives: (i) Identify the differential expression profile of the transcript and microRNA sequences in each parental genotype (pacu and tambaqui) compared to hybrid (tambacu); (ii) evaluate of similarity degree between the nucleotide sequences among genotypes; (iii) performing the signalling pathways prediction and construction of microRNA-mRNA interaction networks involved with the development, growth and maintenance of muscle phenotype among genotypes; (iv) identify potential biomarkers or genetic targets related to hybrid vigor presented by tambacu; (v) evaluate the expression of microRNA and the main transcripts through RTqPCR, and the biomarkers identified after comparing the three genotypes; (vi) establish the primary culture of myoblasts in tambaqui and tambacu and (vii) corroborating data obtained from the differential expression profiling, prediction signaling pathways and networks of microRNA-mRNA interaction (in silico analysis) among the three genotypes in cell culture of myoblasts. Our results will contribute to a better understanding of the biological processes related to the higher growth rate of tambaqui and the better adaptation to low temperatures observed in pacu. Considering the more specific results with better applicability we expected to identify potential biomarkers and/or targets gene related to hybrid vigor presented by tambacu. Also, relevant data regarding the pattern of mRNAs and microRNAs expression between genotypes will be obtained, with emphasis on the sequences related to muscle growth. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE PAULA, TASSIANA GUTIERREZ; THOMAZINI ZANELLA, BRUNA TEREZA; DE MORAES, LEONARDO NAZARIO; VALENTE, JESSICA SILVINO; NASCIMENTO-OLIVEIRA, JORDANA INACIO; MARECO, EDSON ASSUNCAO; FERNANDEZ, GEYSSON JAVIER; DAL-PAI-SILVA, MAELI. egree of piRNA sharing and Piwi gene expression in the skeletal muscle of Piaractus mesopotamicus (pacu), Colossoma macropomum (tambaqui), and the hybrid tambac. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v. 264, FEB 2022. Web of Science Citations: 0.

FANTINATTI, BRUNO E. A.; PEREZ, ERIKA S.; ZANELLA, BRUNA T. T.; VALENTE, JESSICA S.; DE PAULA, TASSIANA G.; MARECO, EDSON A.; CARVALHO, ROBSON F.; PIAZZA, SILVANO; DENTI, MICHELA A.; DAL-PAI-SILVA, MAELI. Integrative microRNAome analysis of skeletal muscle of Colossoma macropomum (tambaqui), Piaractus mesopotamicus (pacu), and the hybrid tambacu, based on next-generation sequencing data. BMC Genomics, v. 22, n. 1 APR 6 2021. Web of Science Citations: 0.

DA SILVA DURAN, BRUNO OLIVEIRA; DAL-PAI-SILVA, MAELI; GARCIA DE LA SERRANA, DANIEL. Rainbow trout slow myoblast cell culture as a model to study slow skeletal muscle, and the characterization of mir-133 and mir-499 families as a case study. Journal of Experimental Biology, v. 223, n. 2 JAN 2020. Web of Science Citations: 3.

SILVA DURAN, BRUNO OLIVEIRA; GOES, GUILHERME ALCARAS; THOMAZINI ZANELLA, BRUNA TEREZA; FREIRE, PAULA PACCIELLI; VALENTE, JESSICA SILVINO; SIMOES SALOMAO, RONDINELLE ARTUR; FERNANDES, ANA; MARECO, EDSON ASSUNCAO; CARVALHO, ROBSON FRANCISCO; DAL-PAI-SILVA, MAELI. Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus). SCIENTIFIC REPORTS, v. 9, FEB 18 2019. Web of Science Citations: 2.