S-nitrosothiol containing nanoparticles: Synthesis, characterization, cytotoxicity and applications

• Grant number: 16/10347-6
• Support Opportunities: Regular Research Grants
• Duration: August 01, 2016 - July 31, 2018
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Amedea Barozzi Seabra
• Grantee: Amedea Barozzi Seabra
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil

Abstract

The free radical nitric oxide (NO) plays a key role in several biological processes in mammals, such as the control of vasodilation, the immunological responses, the inhibition of platelet adhesion, the cell communication, and the wound healing. In addition, NO is recognized as important molecule in several physiologic process in plants, including break seed dormancy, stimulate plant germination and greening, control iron homeostasis in plants, and improve plant tolerance to salinity, metal toxicity, temperature and drought stress. As a free radical, NO donors are administrated in several applications. Among them, S-nitrosothiols (RSNOs) are an important class of NO donors, since they spontaneously decompose releasing NO, and this decomposition can be triggered by light irradiation (visible region). Several papers in the literature reveal that the combination of nanotechnology and RSNOs is a promising strategy to allow the therapeutic uses of NO. In this context, the main goal of this project is the synthesis and characterization of RSNOs containing (i) biocompatible polymeric based nanoparticles (poly(lactic-co-glycolic acid), quitosan and polycaprolactone); (ii) ferromagnetic nanoparticles (Fe3O4@quitosana). The kinetics of thermal and photochemical NO release from the synthesized nanoparticles and the cytotoxicity and mechanisms of toxicity will be evaluated. Moreover, the NO-releasing nanomaterials will be applied on plants under abiotic stresses. The project hopes to contribute to the design of biocompatible NO-releasing nanomaterials for biomedical and agriculture applications. (AU)

Scientific publications

(16)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PELEGRINO, MILENA T.; PAGANOTTI, ANDRE; SEABRA, AMEDEA B.; WELLER, RICHARD B.. Photochemistry of nitric oxide andS-nitrosothiols in human skin. Histochemistry and Cell Biology, v. 153, n. 6, p. 431-441, JUN 2020. (16/10347-6, 17/05029-8)

PELEGRINO, M. T.; BALDI, C.; SOUZA, A. C. S.; SEABRA, A. B.; IOP. Cytotoxicity of hyaluronic acid coated chitosan nanoparticles containing nitric oxide donor against cancer cell lines. WAKE CONFERENCE 2021, v. 1323, p. 8-pg., 2019-01-01. (16/10347-6, 17/05029-8, 18/13761-3)

NELSON DURÁN; WALLACE R. ROLIM; MARCELA DURÁN; WAGNER J. FÁVARO; AMEDEA B. SEABRA. NANOTOXICOLOGIA DE NANOPARTÍCULAS DE PRATA: TOXICIDADE EM ANIMAIS E HUMANOS. Química Nova, v. 42, n. 2, p. 206-213, 2019-02-00. (16/10347-6)

GONCALVES, L. C.; SEABRA, A. B.; PELEGRINO, M. T.; DE ARAUJO, D. R.; BERNARDES, J. S.; HADDAD, P. S.. Superparamagnetic iron oxide nanoparticles dispersed in Pluronic F127 hydrogel: potential uses in topical applications. RSC ADVANCES, v. 7, n. 24, p. 14496-14503, 2017. (16/10347-6, 14/13913-7)

PELEGRINO, M. T.; WELLER, R. B.; CHEN, X.; BERNARDES, J. S.; SEABRA, A. B.. Chitosan nanoparticles for nitric oxide delivery in human skin. MedChemComm, v. 8, n. 4, p. 713-719, APR 1 2017. (16/10347-6, 15/00393-8)

OLIVEIRA, HALLEY C.; GOMES, BRUNA C. R.; PELEGRINO, MILENA T.; SEABRA, AMEDEA B.. Nitric oxide-releasing chitosan nanoparticles alleviate the effects of salt stress in maize plants. NITRIC OXIDE-BIOLOGY AND CHEMISTRY, v. 61, p. 10-19, DEC 30 2016. (16/10347-6, 15/00393-8)

HADDAD, PAULA S.; SANTOS, MARCONI C.; DE GUZZI CASSAGO, CAROLINA APARECIDA; BERNARDES, JULIANA S.; DE JESUS, MARCELO BISPO; SEABRA, AMEDEA B.. Synthesis, characterization, and cytotoxicity of glutathione-PEG-iron oxide magnetic nanoparticles. JOURNAL OF NANOPARTICLE RESEARCH, v. 18, n. 12, DEC 10 2016. (16/10347-6, 14/13913-7)

ROLIM, WALLACE R.; PIERETTI, JOANA C.; RENO, DEBORA L. S.; LIMA, BRUNA A.; NASCIMENTO, MONICA H. M.; AMBROSIO, FELIPE N.; LOMBELLO, CHRISTIANE B.; BROCCHI, MARCELO; DE SOUZA, ANA CAROLINA S.; SEABRA, AMEDEA B.. Antimicrobial Activity and Cytotoxicity to Tumor Cells of Nitric Oxide Donor and Silver Nanoparticles Containing PVA/PEG Films for Topical Applications. ACS APPLIED MATERIALS & INTERFACES, v. 11, n. 6, p. 6589-6604, FEB 13 2019. (16/10347-6, 18/02832-7)

PELEGRINO, MILENA T.; SILVA, LETECIA C.; WATASHI, CAROLINA M.; HADDAD, PAULA S.; RODRIGUES, TIAGO; SEABRA, AMEDEA B.. Nitric oxide-releasing nanoparticles: synthesis, characterization, and cytotoxicity to tumorigenic cells. JOURNAL OF NANOPARTICLE RESEARCH, v. 19, n. 2, FEB 8 2017. (16/10347-6, 15/00393-8, 14/13913-7)

PELEGRINO, MILENA T.; LIMA, BRUNA DE ARAUJO; DO NASCIMENTO, MONICA H. M.; LOMBELLO, CHRISTIANE B.; BROCCHI, MARCELO; SEABRA, AMEDEA B.. Biocompatible and Antibacterial Nitric Oxide-Releasing Pluronic F-127/Chitosan Hydrogel for Topical Applications. POLYMERS, v. 10, n. 4, APR 2018. (16/10347-6, 17/05029-8)

SEABRA, AMEDEA B.; OLIVEIRA, HALLEY C.. How nitric oxide donors can protect plants in a changing environment: what we know so far and perspectives. AIMS MOLECULAR SCIENCE, v. 3, n. 4, p. 692-718, 2016. (16/10347-6)

PELEGRINO, MILENA T.; DE ARAUJO, DANIELE R.; SEABRA, AMEDEA B.. S-nitrosoglutathione-containing chitosan nanoparticles dispersed in Pluronic F-127 hydrogel: Potential uses in topical applications. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, v. 43, p. 211-220, FEB 2018. (16/10347-6, 15/00393-8, 14/13913-7)

PELEGRINO, MILENA T.; SEABRA, AMEDEA B.. Chitosan-Based Nanomaterials for Skin Regeneration. AIMS MEDICAL SCIENCE, v. 4, n. 3, p. 352-381, 2017. (16/10347-6, 17/05029-8)

ROLIM, WALLACE R.; PELEGRINO, MILENA T.; LIMA, BRUNA DE ARAUJO; FERRAZ, LETICIA S.; COSTA, FANNY N.; BERNARDES, JULIANA S.; RODIGUES, TIAGO; BROCCHI, MARCELO; SEABRA, AMEDEA B.. Green tea extract mediated biogenic synthesis of silver nanoparticles: Characterization, cytotoxicity evaluation and antibacterial activity. Applied Surface Science, v. 463, p. 66-74, JAN 1 2019. (16/10347-6)

SILVEIRA, NEIDIQUELE M.; SEABRA, AMEDEA B.; MARCOS, FERNANDA C. C.; PELEGRINO, MILENA T.; MACHADO, EDUARDO C.; RIBEIRO, RAFAEL V.. Encapsulation of S-nitrosoglutathione into chitosan nanoparticles improves drought tolerance of sugarcane plants. NITRIC OXIDE-BIOLOGY AND CHEMISTRY, v. 84, p. 38-44, MAR 1 2019. (16/10347-6, 18/08194-2, 12/19167-0, 15/00393-8)

LOURENCO, I. M.; PELEGRINO, M. T.; PIERETTI, J. C.; ANDRADE, G. P.; CERCHIARO, G.; SEABRA, A. B.; IOP. Synthesis, characterization and cytotoxicity of chitosan-coated Fe3O4 nanoparticles functionalized with ascorbic acid for biomedical applications. WAKE CONFERENCE 2021, v. 1323, p. 10-pg., 2019-01-01. (18/02832-7, 16/10347-6, 18/13761-3, 16/09652-9, 17/05029-8)

Filed patent(s) as a result of this research project