Abstract
The free radical nitric oxide (NO) plays a key role in several biological processes in mammals, such as the control of vasodilation, the immunological responses, the inhibition of platelet adhesion, the cell communication, and the wound healing. In addition, NO is recognized as important molecule in several physiologic process in plants, including break seed dormancy, stimulate plant germination and greening, control iron homeostasis in plants, and improve plant tolerance to salinity, metal toxicity, temperature and drought stress. As a free radical, NO donors are administrated in several applications. Among them, S-nitrosothiols (RSNOs) are an important class of NO donors, since they spontaneously decompose releasing NO, and this decomposition can be triggered by light irradiation (visible region). Several papers in the literature reveal that the combination of nanotechnology and RSNOs is a promising strategy to allow the therapeutic uses of NO. In this context, the main goal of this project is the synthesis and characterization of RSNOs containing (i) biocompatible polymeric based nanoparticles (poly(lactic-co-glycolic acid), quitosan and polycaprolactone); (ii) ferromagnetic nanoparticles (Fe3O4@quitosana). The kinetics of thermal and photochemical NO release from the synthesized nanoparticles and the cytotoxicity and mechanisms of toxicity will be evaluated. Moreover, the NO-releasing nanomaterials will be applied on plants under abiotic stresses. The project hopes to contribute to the design of biocompatible NO-releasing nanomaterials for biomedical and agriculture applications. (AU)
Scientific publications
(14)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ROLIM, WALLACE R.;
PIERETTI, JOANA C.;
RENO, DEBORA L. S.;
LIMA, BRUNA A.;
NASCIMENTO, MONICA H. M.;
AMBROSIO, FELIPE N.;
LOMBELLO, CHRISTIANE B.;
BROCCHI, MARCELO;
DE SOUZA, ANA CAROLINA S.;
SEABRA, AMEDEA B.
Antimicrobial Activity and Cytotoxicity to Tumor Cells of Nitric Oxide Donor and Silver Nanoparticles Containing PVA/PEG Films for Topical Applications.
ACS APPLIED MATERIALS & INTERFACES,
v. 11,
n. 6,
p. 6589-6604,
FEB 13 2019.
Web of Science Citations: 5.
ROLIM, WALLACE R.;
PELEGRINO, MILENA T.;
LIMA, BRUNA DE ARAUJO;
FERRAZ, LETICIA S.;
COSTA, FANNY N.;
BERNARDES, JULIANA S.;
RODIGUES, TIAGO;
BROCCHI, MARCELO;
SEABRA, AMEDEA B.
Green tea extract mediated biogenic synthesis of silver nanoparticles: Characterization, cytotoxicity evaluation and antibacterial activity.
Applied Surface Science,
v. 463,
p. 66-74,
JAN 1 2019.
Web of Science Citations: 35.
PELEGRINO, MILENA T.;
SILVA, LETECIA C.;
WATASHI, CAROLINA M.;
HADDAD, PAULA S.;
RODRIGUES, TIAGO;
SEABRA, AMEDEA B.
Nitric oxide-releasing nanoparticles: synthesis, characterization, and cytotoxicity to tumorigenic cells.
JOURNAL OF NANOPARTICLE RESEARCH,
v. 19,
n. 2
FEB 8 2017.
Web of Science Citations: 12.
GONCALVES, L. C.;
SEABRA, A. B.;
PELEGRINO, M. T.;
DE ARAUJO, D. R.;
BERNARDES, J. S.;
HADDAD, P. S.
Superparamagnetic iron oxide nanoparticles dispersed in Pluronic F127 hydrogel: potential uses in topical applications.
RSC ADVANCES,
v. 7,
n. 24,
p. 14496-14503,
2017.
Web of Science Citations: 19.
HADDAD, PAULA S.;
SANTOS, MARCONI C.;
DE GUZZI CASSAGO, CAROLINA APARECIDA;
BERNARDES, JULIANA S.;
DE JESUS, MARCELO BISPO;
SEABRA, AMEDEA B.
Synthesis, characterization, and cytotoxicity of glutathione-PEG-iron oxide magnetic nanoparticles.
JOURNAL OF NANOPARTICLE RESEARCH,
v. 18,
n. 12
DEC 10 2016.
Web of Science Citations: 6.