| Grant number: | 15/50451-4 |
| Support type: | Regular Research Grants |
| Duration: | July 01, 2016 - June 30, 2018 |
| Field of knowledge: | Biological Sciences - Biochemistry - Molecular Biology |
| Cooperation agreement: | Texas Tech University |
| Mobility Program: | SPRINT - Projetos de pesquisa - Mobilidade |
| Principal Investigator: | Sonia Marli Zingaretti |
| Grantee: | Sonia Marli Zingaretti |
| Principal investigator abroad: | Kameswara Rao Kottapalli |
| Institution abroad: | Texas Tech University (TTU), United States |
| Home Institution: | Universidade de Ribeirão Preto (UNAERP). Campus Ribeirão Preto. Ribeirão Preto , SP, Brazil |
| Associated research grant: | 14/19667-8 - MiRNAs expression analysis in sugarcane under biotic and abiotic stresses, AP.R |
Abstract
The sugarcane is currently one the major crops of the Brazilian and global agribusiness, with has led to a great expansion of cultivated areas with different soil and climatic conditions. One of problems faced in sugarcane cultivation is the presence of toxic molecules such as aluminium (Al3+) in the soil, which interferes with root development and ultimately compromising the overall plant development. Besides abiotic factors sugarcane are also affected by biotic factors such as "leaf scald" disease caused by gram-negative bacteria Xanthomonas albilineans, considered one of the most important diseases of sugarcane. The symptomatology int the chronic phase is characterized by the appearance of a while band across the leaf, which evolves until it is fully burned. The development of tolerant cultivars is always the bet option for disease control. The sensitivity and tolerance to biotic and abiotic factors are related with the expression of genes that along with miRNAs have been shown to be important regulators in several mechanims related to plants adaptation in different stresses. The objective of this project validate and analyze the expression of miRNAs and their target genes in response to biotic and abiotic stresses will perform RNA-Seq experiments on both mRNAand miRNA to identify target genes and their corresponding regulatory miRNAs. Analysis of the expression of microRNAs along with their target genes may provide better understanding of mechanimsunderlying plant development and tolerance or sensivity to stresses. (AU)
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