Involvement of socs-jak-stat signalling in the effects of aerobic exercise in a model of asthma

• Grant number: 16/50010-0
• Support type: Regular Research Grants
• Duration: August 01, 2016 - July 31, 2018
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Cooperation agreement: Imperial College, UK
• Mobility Program: SPRINT - Projetos de pesquisa - Mobilidade
• Principal researcher: Rodolfo de Paula Vieira
• Grantee: Rodolfo de Paula Vieira
• Principal researcher abroad: Maria Gabriela Belvisi
• Institution abroad: Imperial College London, England
• Home Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil
• Associated research grant: 12/15165-2 - Role of purinergic signaling and of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic training in experimental models of asma and in asthmatic individuals, AP.JP

Abstract

Asthma is a chronic airway inflammatory disease that results in impairment of quality of rife. In the last years, several studies have demonstrated that aerobic training (AT) performed properly results in anti-inflammatory effects to the airways, either in experimental models of asthma and to asthmatic individuals. However, although few cellular effects have been investigated (still only in animals’ models), the possible extra and intracellular mediators underlying the effects of AT on asthma never were investigated, except to NF-kB. Thus, the present project aims to evaluate the effects of AT on SOCS, JAK and STAT proteins signaling on the lungs as possible modulators of the anti-inflammatory effects of aerobic training in an experimental model of asthma (by using house dust mite HDM). For this purpose, the animals will receive intra-tracheal administration of FIDM (10Oug) on days 0, 7, 14, 21, 28, 35 e 42 and will be submitted to AT (for evaluation of therapeutic effect) beginning on day 17 during 5 weeks. Twenty-four hours after the last session of training and allergen challenge, the animals will be assessed to the inflammatory profile in the bronchoalveolar lavage and in the lung tissue, ta the levels of pro-inflammatory cytokines (IL-1 beta, IL-2, IL-4, 1L-5, 1L-6, IL-8, IL-12, IL-13, IL-17, 1L-23) and to the levels of anti-inflammatory cytokines (1L-10, IL-1ra) and also for the expression of SOCS1, SOCS2, SOCS3, JAK2, phospho JAK2, STAT3, phospho STAT3, STAT5, phospho STAT5, STAT6 and phospho STAT6 in the lungs, as well as for the airway remodeling, and bronchial. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RODRIGUES BRANDAO-RANGEL, MAYSA ALVES; LACERDA BACHI, ANDRE LUIS; OLIVEIRA-JUNIOR, MANOEL CARNEIRO; ABBASI, ASGHAR; SILVA-RENNO, ADRIANO; DE BRITO, AURILEIA APARECIDA; LIGEIRO DE OLIVEIRA, ANA PAULA; TOLEDO-ARRUDA, ALESSANDRA CHOQUETA; BELVISI, MARIA GABRIELA; VIEIRA, RODOLFO PAULA. Exercise Inhibits the Effects of Smoke-Induced COPD Involving Modulation of STAT3. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2017. (14/14604-8, 12/15165-2, 16/50010-0)