Neutrophil bactericidal killing and phagocytosis of distinct bovine-associated staphylococci species and strains in primiparous and multiparous dairy cows

• Grant number: 15/16871-6
• Support Opportunities: Regular Research Grants
• Duration: August 01, 2016 - July 31, 2018
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Alice Maria Melville Paiva Della Libera
• Grantee: Alice Maria Melville Paiva Della Libera
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Cristina de Oliveira Massoco Salles Gomes ; Fernando Nogueira de Souza ; Luciana Bignardi de Soares Brisola Casimiro da Costa ; Maiara Garcia Blagitz ; Marcos Bryan Heinemann ; Mônica Maria Oliveira Pinho Cerqueira ; Sarne de Vliegher ; Sofie Piepers

Abstract

Mastitis affects not just the profitability of the farm but also is associated with animal welfare concerns, public health risk due to antimicrobial residues and emergence of bacterial resistance. Striving for a complete elimination of antimicrobial use in dairy cattle is unrealistic though a reduction is recommended and feasible. From that point of view, innovative tools enabling an increase in the natural ability of animals to resist infection could be of great value. The greatest obstacle in establishing this kind of strategies is a lack of understanding of many aspects of the interaction among mastitis-pathogens species and strains and the host. Neutrophils are essential for innate host defense against invading microorganisms and eliminate pathogens. Staphylococcal species represent an important cause of bovine mastitis, and there is a gap in our knowledge on the their epidemiology, virulence and interaction with host. The genus is usually divided into the coagulase-positive staphylococci with S. aureus remaining the most significant mastitis pathogen among the staphylococci and the heterogenous group of coagulase-negative staphylococci which have become the most commonly isolated bacterial pathogens from milk samples of dairy cows and heifers in many regions and countries. Thus, the proposed project aimed at explore the difference in the ability of bovine-associated staphylococcal species and strains to resist phagocytosis and bacterial killing by bovine neutrophils. Here, the neutrophil function (phagocytosis, bacterial killing, the release of extracellular traps and viability) of primiparous and multiparous cows will also be investigated. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOUZA, RODRIGO M.; SOUZA, FERNANDO N.; BATISTA, CAMILA F.; PIEPERS, SOFIE; DE VISSCHER, ANNELEEN; SANTOS, KAMILA R.; MOLINARI, PAULA C.; FERRONATTO, JOSE A.; DA CUNHA, ADRIANO FRANCA; BLAGITZ, MAIARA G.; et al. Distinct behavior of bovine-associated staphylococci species in their ability to resist phagocytosis and trigger respiratory burst activity by blood and milk polymorphonuclear leukocytes in dairy cow. JOURNAL OF DAIRY SCIENCE, v. 105, n. 2, p. 1625-1637, FEB 2022. (16/06297-3, 17/07921-5, 18/03685-8, 15/16871-6, 14/23189-4)