Chronic stress and adrenergic signaling in the progression of periapicopatia: a potential therapeutic target?

• Grant number: 16/04711-7
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2016
• End date: August 31, 2018
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Cláudio Antonio Talge Carvalho
• Grantee: Cláudio Antonio Talge Carvalho
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil
• Associated researchers: Marcia Carneiro Valera Garakis ; Renata Falchete Do Prado

Abstract

This study will evaluate the importance of chronic stress (EC) in stimulation of the sympathetic nervous system and explore the pathogenic mechanisms underlying periapical lesions. Animal model of chronic stress and periapical lesion as well as cultured human fibroblasts will be used. The expression of tyrosine hydroxylase, the ±1-adrenergic (±1-AR) and ²2-adrenergic (²2-AR) receptors will be evaluated. In addition, the human periodontal ligament fibroblasts will be stimulated with lipopolysaccharide (LPS) to mimic the process of inflammation. The viability and the expression of ±1-AR and ²2-AR will be assessed. Inflammatory cytokines: Interleukin (IL) IL-1², IL-6, IL-10 and IL17 will be detected after pre-treatment with the adrenergic receptors blockers: phentolamine / propranolol, both in vitro and in vivo. The data will be evaluated by descriptive and inferential statistical tests, according to data obtained, according to their distribution, with parametric or non-parametric tests. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KHOURY, RAYANA DUARTE; PRADO, RENATA FALCHETE; MATOS, FELIPE DE SOUZA; DE MEIRELES, BRUNA RIBAS; DA ROSA CARDOSO, FLAVIA GOULART; DE OLIVEIRA, LUCIANE DIAS; TALGE CARVALHO, CLAUDIO ANTONIO; VALERA, MARCIA CARNEIRO. The influence of adrenergic blockade in rats with apical periodontitis under chronic stress conditions. ARCHIVES OF ORAL BIOLOGY, v. 110, FEB 2020. (16/05821-0, 16/04711-7)