Expanding and barcoding the trypanosomatid culture collection of the University of São Paulo (TCC-USP) and its utilization for phylogenetic and taxonomical studies

• Grant number: 16/07487-0
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2016
• End date: July 31, 2018
• Field of knowledge: Biological Sciences - Parasitology - Protozoology of Parasites
• Principal Investigator: Erney Felicio Plessmann de Camargo
• Grantee: Erney Felicio Plessmann de Camargo
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Marta Maria Geraldes Teixeira

Abstract

Summary. The importance of the Trypanosomatidae family relies not only on the human parasites responsible for debilitating and lethal endemic diseases in the Americas and Africa. The contemporary importance of the species of this family also rests on its richness of models for studies of evolution, cell development, biogeography and phylogeography of protists in general. The main goals of this project are: (1) to enlarge the number of cultures of the Trypanosomatid Culture Collection of the University of São Paulo (TCC-USP) currently housing ~2500 cultures in N2-liquid from a high diversity of vertebrates (fishes, amphibians, reptiles, birds and mammals, including humans), and invertebrates (Diptera, Hemiptera and leeches); (2) the barcoding of the entire culture collection by sequencing the SSU rRNA and gGAPDH genes; (3) the extension of studies on the diversity, biogeography, phylogeny and taxonomy of the Trypanosomatidae initiated in our laboratory more than 20 years ago. For these purposes, ~ 500 trypanosomatid samples from different Brazilian biomes and other Neotropical and Afrotropical regions will be barcoded, and complete genomes of selected trypanosomatids determined for phylogenetic and evolutionary studies.Key words: Trypanosomatidae, parasites, insect, plant, DNA barcoding, phylogeny, taxonomy. (AU)

Scientific publications

(14)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RODRIGUES, CARLA M. F.; GARCIA, HERAKLES A.; RODRIGUES, ADRIANA C.; COSTA-MARTINS, ANDRE G.; PEREIRA, CARLOS L.; PEREIRA, DAGMAR L.; BENGALY, ZAKARIA; NEVES, LUIS; CAMARGO, ERNEY P.; HAMILTON, PATRICK B.; et al. New insights from Gorongosa National Park and Niassa National Reserve of Mozambique increasing the genetic diversity of Trypanosoma vivax and Trypanosoma vivax-like in tsetse flies, wild ungulates and livestock from East Africa. PARASITES & VECTORS, v. 10, JUL 17 2017. (16/03028-1, 16/07487-0)

GARCIA, HERAKLES A.; RODRIGUES, CARLA M. F.; RODRIGUES, ADRIANA C.; PEREIRA, DAGMAR L.; PEREIRA, CARLOS L.; CAMARGO, ERNEY P.; HAMILTON, P. B.; TEIXEIRA, MARTA M. G.. Remarkable richness of trypanosomes in tsetse flies (Glossina morsitans morsitans and Glossina pallidipes) from the Gorongosa National Park and Niassa National Reserve of Mozambique revealed by fluorescent fragment length barcoding (FFLB). INFECTION GENETICS AND EVOLUTION, v. 63, p. 370-379, SEP 2018. (16/03028-1, 16/07487-0)

RODRIGUES, CARLA M. F.; GARCIA, HERAKLES A.; RODRIGUES, ADRIANA C.; PEREIRA, DAGMAR LOPES; PEREIRA, CARLOS LOPES; VIOLA, LAERTE B.; NEVES, LUIS; CAMARGO, ERNEY P.; GIBSON, WENDY; TEIXEIRA, MARTA M. G.. Expanding our knowledge on African trypanosomes of the subgenus Pycnomonas: A novel Trypanosoma suis-like in tsetse flies, livestock and wild ruminants sympatric with Trypanosoma suis in Mozambique. INFECTION GENETICS AND EVOLUTION, v. 78, MAR 2020. (16/07487-0)

BORGHESAN, TARCILLA C.; CAMPANER, MARTA; MATSUMOTO, TANIA E.; ESPINOSA, OMAR A.; RAZAFINDRANAIVO, VICTOR; PAIVA, FERNANDO; CARRANZA, JULIO C.; ANEZ, NESTOR; NEVES, LUIS; TEIXEIRA, MARTA M. G.; et al. Genetic Diversity and Phylogenetic Relationships of Coevolving Symbiont-Harboring Insect Trypanosomatids, and Their Neotropical Dispersal by Invader African Blowflies (Calliphoridae). FRONTIERS IN MICROBIOLOGY, v. 9, FEB 7 2018. (16/07487-0)

GARCIA, HERAKLES A.; BLANCO, PILAR A.; RODRIGUES, ADRIANA C.; RODRIGUES, CARLA M. F.; TAKATA, CARMEN S. A.; CAMPANER, MARTA; CAMARGO, ERNEY P.; TEIXEIRA, MARTA M. G.. Pan-American Trypanosoma (Megatrypanum) trinaperronei n. sp. in the white-tailed deer Odocoileus virginianus Zimmermann and its deer ked Lipoptena mazamae Rondani, 1878: morphological, developmental and phylogeographical characterisation. PARASITES & VECTORS, v. 13, n. 1, JUN 12 2020. (16/03028-1, 16/07487-0)

FLORENTIN, ANDREA S.; GARCIA PEREZ, HERAKLES A.; RODRIGUES, CARLA M. F.; DUBOIS, EUGENIO F.; MONZON, CARLOS M.; TEIXEIRA, MARTA M. G.. Molecular epidemiological insights into Trypanosoma vivax in Argentina: From the endemic Gran Chaco to outbreaks in the Pampas. TRANSBOUNDARY AND EMERGING DISEASES, v. 69, n. 3, p. 11-pg., 2021-04-20. (16/07487-0, 16/03028-1)

FLORENTIN, ANDREA S.; GARCIA PEREZ, HERAKLES A.; RODRIGUES, CARLA M. F.; DUBOIS, EUGENIO F.; MONZON, CARLOS M.; TEIXEIRA, MARTA M. G.. Molecular epidemiological insights into Trypanosoma vivax in Argentina: From the endemic Gran Chaco to outbreaks in the Pampas. TRANSBOUNDARY AND EMERGING DISEASES, APR 2021. (16/07487-0, 16/03028-1)

ESPINOSA-ALVAREZ, ONEIDA; ORTIZ, PAOLA A.; LIMA, LUCIANA; COSTA-MARTINS, ANDRE G.; SERRANO, MYRNA G.; HERDER, STEPHANE; BUCK, GREGORY A.; CAMARGO, ERNEY P.; HAMILTON, PATRICK B.; STEVENS, JAMIE R.; et al. Trypanosoma rangeli is phylogenetically closer to Old World trypanosomes than to Trypanosoma cruzi. International Journal for Parasitology, v. 48, n. 7, p. 569-584, JUN 2018. (16/07487-0)

SKALICKY, TOMAS; ALVES, JOAO M. P.; MORAIS, ANDERSON C.; REZNAROVA, JANA; BUTENKO, ANZHELIKA; LUKES, JULIUS; SERRANO, MYRNA G.; BUCK, GREGORY A.; TEIXEIRA, MARTA M. G.; CAMARGO, ERNEY P.; et al. Endosymbiont Capture, a Repeated Process of Endosymbiont Transfer with Replacement in Trypanosomatids Angomonas spp.. PATHOGENS, v. 10, n. 6, JUN 2021. (16/07487-0, 13/14622-3)

GARCIA PEREZ, HERAKLES ANTONIO; FILGUEIRA RODRIGUES, CARLA MONADELI; VIVAS PIVAT, ISIS HELGA; RODRIGUES FUZATO, ADRIANA CARLOS; CAMARGO, ERNEY P.; HAMAD MINERVINO, ANTONIO HUMBERTO; GERALDES TEIXEIRA, MARTA MARIA. High Trypanosoma vivax infection rates in water buffalo and cattle in the Brazilian Lower Amazon. Parasitology International, v. 79, p. 9-pg., 2020-12-01. (16/07487-0, 16/03028-1)

COSTA-MARTINS, ANDRE G.; LIMA, LUCIANA; ALVES, JOAO MARCELO P.; SERRANO, MYRNA G.; BUCK, GREGORY A.; CAMARGO, ERNEY P.; TEIXEIRA, MARTA M. G.. Genome-wide identification of evolutionarily conserved Small Heat-Shock and eight other proteins bearing alpha-crystallin domain-like in kinetoplastid protists. PLoS One, v. 13, n. 10, OCT 22 2018. (16/07487-0)

FERMINO, BRUNO R.; PAIVA, FERNANDO; VIOLA, LAERTE B.; RODRIGUES, CARLA M. F.; GARCIA, HERAKLES A.; CAMPANER, MARTA; TAKATA, CARMEN S. A.; SHEFERAW, DESIE; KISAKYE, JOHN J.; KATO, AGAPITUS; et al. Shared species of crocodilian trypanosomes carried by tabanid flies in Africa and South America, including the description of a new species from caimans, Trypanosoma kaiowa n. sp.. PARASITES & VECTORS, v. 12, MAY 14 2019. (16/07487-0)

RODRIGUES, CARLA M. F.; GARCIA, HERAKLES A.; SHEFERAW, DESIE; RODRIGUES, ADRIANA C.; PEREIRA, CARLOS L.; CAMARGO, ERNEY P.; TEIXEIRA, MARTA M. G.. Genetic diversity of trypanosomes pathogenic to livestock in tsetse flies from the Nech Sar National Park in Ethiopia: A concern for tsetse suppressed area in Southern Rift Valley?. INFECTION GENETICS AND EVOLUTION, v. 69, p. 38-47, APR 2019. (16/03028-1, 16/07487-0)

ORTIZ, PAOLA A.; GARCIA, HERAKLES A.; LIMA, LUCIANA; DA SILVA, FLAVIA MAIA; CAMPANER, MARTA; PEREIRA, CARLOS L.; JITTAPALAPONG, SATHAPORN; NEVES, LUIS; DESQUESNES, MARC; CAMARGO, ERNEY P.; et al. Diagnosis and genetic analysis of the worldwide distributed Rattus-borne Trypanosoma (Herpetosoma) lewisi and its allied species in blood and fleas of rodents. INFECTION GENETICS AND EVOLUTION, v. 63, p. 380-390, SEP 2018. (16/03028-1, 16/07487-0)