Chemical analyze and therapeutic prospection of Syngonanthus nitens (Bong.) Ruhland and Cymbopogon nardus (L.) Rendle loaded into nanotechnological systems applied to vulvovaginal candidiasis treatment

• Grant number: 16/08559-5
• Support Opportunities: Regular Research Grants
• Duration: September 01, 2016 - August 31, 2018
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Taís Maria Bauab
• Grantee: Taís Maria Bauab
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated researchers: André Gonzaga dos Santos ; Margarete Teresa Gottardo de Almeida ; Marlus Chorilli

Abstract

The incidence of fungal infections, especially those caused by Candida yeasts, has increased in recent decades. Vulvovaginal candidiasis (VVC) is an opportunistic infection that causes significant problem to women's health. These yeasts present several virulence factors, such as, biofilm formation, morphological transition between yeast and hyphae, adhesion and production of hydrolytic enzymes and others. The therapy for control of fungal infections, especially to azole compounds, polyene and echinocandins, has limitations such as high cost, high toxicity, drug interactions, inadequate bioavailability of the active ingredient, in addition to the emergence of resistant strains. In this sense, the medicinal plants are an alternative in the search for new antifungal agents. Syngonanthus nitens (Bong.) Ruhland and Cymbopogon nardus (L.) Rendle has shown promising results against pathogenic fungi of the genus Candida. Additionally, nanotechnology is presented as a tool to antifungal activity of natural products. In this study we aimed to evaluate the antifungal potential of these plant species employing the pharmaceutical nanotechnology as a tool to enhance the action of plant derived against three species of yeast: C. albicans, C. krusei and C. glabrata, focusing the control of the VVC. The activity of plant derivatives loaded in nanostructured systems of drug delivery (lipid microemulsion) will be determinated in order to improve its antifungal action. In vitro assays will be employed with emphasis on monotypical biofilms (C. albicans, C. glabrata and C. krusei) and heterotypic (+ Lactobacillus sp) and in vivo assays will be performed with Wistar rats (Rattus norvegicus) (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARENA, GABRIEL DAVI; GIROTTO, LUIZA; SALDANHA, LUIZ LEONARDO; RAMOS, MATHEUS APARECIDO DOS SANTOS; DE GRANDIS, RONE APARECIDO; DA SILVA, PATRICIA BENTO; DOKKEDAL, ANNE LIGIA; CHORILLI, MARLUS; BAUAB, TAIS MARIA; PAVAN, FERNANDO ROGERIO; et al. Hydroalcoholic Extract of Myrcia bella Loaded into a Microemulsion System: A Study of Antifungal and Mutagenic Potential. Planta Medica, JAN 2021. (16/08559-5, 17/16278-9, 18/12590-0, 17/18782-6)

SPOSITO, LARISSA; ODA, FERNANDO BOMBARDA; VIEIRA, JULIA HUNGER; CARVALHO, FLAVIO ALEXANDRE; DOS SANTOS RAMOS, MATHEUS APARECIDO; DE CASTRO, ROGERIO CARDOSO; CREVELIN, EDUARDO JOSE; MILLER CROTTI, ANTONIO EDUARDO; SANTOS, ANDRE GONZAGA; DA SILVA, PATRICIA BENTO; et al. In vitro and in vivo anti-Helicobacter pylori activity of Casearia sylvestris leaf derivatives. Journal of Ethnopharmacology, v. 233, p. 1-12, APR 6 2019. (16/08559-5)

MARENA, GABRIEL DAVI; GIROTTO, LUIZA; SALDANHA, LUIZ LEONARDO; RAMOS, MATHEUS APARECIDO DOS SANTOS; DE GRANDIS, RONE APARECIDO; DA SILVA, PATRICIA BENTO; DOKKEDAL, ANNE LIGIA; CHORILLI, MARLUS; BAUAB, TAIS MARIA; PAVAN, FERNANDO ROGERIO; et al. Hydroalcoholic Extract of Myrcia bella Loaded into a Microemulsion System: A Study of Antifungal and Mutagenic Potential. Planta Medica, v. 88, n. 05, p. 11-pg., 2021-01-28. (17/18782-6, 17/16278-9, 18/12590-0, 16/08559-5)

DOS SANTOS RAMOS, MATHEUS APARECIDO; DA SILVA, PATRICIA BENTO; DE TOLEDO, LUCIANI GASPAR; ODA, FERNANDO BOMBARDA; DA SILVA, ISABEL CRISTIANE; DOS SANTOS, LOURDES CAMPANER; DOS SANTOS, ANDRE GONZAGA; GOTTARDO DE ALMEIDA, MARGARETE TERESA; PAVAN, FERNANDO ROGERIO; CHORILLI, MARIUS; et al. Intravaginal Delivery of Syngonanthus nitens (Bong.) Ruhland Fraction Based on a Nanoemulsion System Applied to Vulvovaginal Candidiasis Treatment. JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, v. 15, n. 5, p. 1072-1089, MAY 2019. (14/24626-9, 16/08559-5)

DE TOLEDO, LUCIANI GASPAR; DOS SANTOS RAMOS, MATHEUS APARECIDO; DA SILVA, PATRICIA BENTO; RODERO, CAMILA FERNANDA; GOMES, VERIDIANA DE SA; DA SILVA, ANDERSON NORONHA; PAVAN, FERNANDO ROGERIO; DA SILVA, ISABEL CRISTIANE; ODA, FERNANDO BOMBARDA; FLUMIGNAN, DANILO LUIS; et al. Improved in vitro and in vivo Anti-Candida albicans Activity of Cymbopogon nardus Essential Oil by Its Incorporation into a Microemulsion System. INTERNATIONAL JOURNAL OF NANOMEDICINE, v. 15, p. 10481-10497, 2020. (16/08559-5, 14/50926-0, 15/23959-7)

LUCIANI G. DE TOLEDO; MATHEUS A. S. RAMOS; LARISSA SPÓSITO; ELZA M. CASTILHO; FERNANDO R. PAVAN; ÉRICA O. LOPES; ISABEL C. DA SILVA; GUILHERME J. ZOCOLO; PAULO R. V. RIBEIRO; FERNANDO B. ODA; et al. Profiling the Cymbopogon nardus Ethanol Extract and Its Antifungal Potential against Candida Species with Different Patterns of Resistance. Journal of the Brazilian Chemical Society, v. 31, n. 9, p. 1926-1938, 2020-08-19. (13/19576-0, 15/23959-7, 16/08559-5)

DOS SANTOS RAMOS, MATHEUS APARECIDO; DA SILVA, PATRICIA BENTO; SPOSITO, LARISSA; DE TOLEDO, LUCIANI GASPAR; BONIFACIO, BRUNA VIDAL; RODERO, CAMILA FERNANDA; DOS SANTOS, KAREN CRISTINA; CHORILLI, MARLUS; BAUAB, TAIS MARIA. Nanotechnology-based drug delivery systems for control of microbial biofilms: a review. INTERNATIONAL JOURNAL OF NANOMEDICINE, v. 13, p. 1179-1213, 2018. (16/08559-5, 14/24626-9)