Abstract
Cellular reprogramming technologies are a promising tool for the understanding of pluripotent cell biology and also to study several genetic and tissue-related disorders, enabling the investigation of the molecular events responsible for the acquisition of competency into pluri- and totipotency. This proposal aims to consolidate procedures for the generation of induced pluripotent stem (iPS) cells and germ cells in vitro from domestic species of commercial and biomedical interest (focus on cattle and swine) by investigating cellular and molecular mechanisms involved in cellular reprogramming, and by disseminating protocols and biological material for collaborations. Three general goals are proposed: 1) The standardization of safe and reproducible protocols of iPS generation, 2) The generation of germ cells, gametes and embryos from in vitro cultured cells, and 3) The investigation of transcriptional and epigenetic mechanisms of cellular reprogramming in domestic animals. The results achieved by this proposal will consolidate national research on nuclear and epigenetic reprogramming, allow the international insertion through scientific collaborations and high impact scientific publications, finally resulting in the formation of a multidisciplinary, competitive and collaborative research group both nationally and internationally. (AU)
Scientific publications
(11)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE CASTRO, RAQUEL V. G.;
PIERI, NAIRA C. G.;
FANTINATO NETO, PAULO;
GRIZENDI, BIANCA M.;
DORIA, RENATA G. S.;
MEIRELLES, FLAVIO V.;
SMITH, LAWRENCE C.;
GARCIA, JOAQUIM M.;
BRESSAN, FABIANA F.
In Vitro Induction of Pluripotency from Equine Fibroblasts in 20% or 5% Oxygen.
STEM CELLS INTERNATIONAL,
v. 2020,
NOV 26 2020.
Web of Science Citations: 0.
MACHADO, LUCAS SIMOES;
PIERI, NAIRA CAROLINE GODOY;
BOTIGELLI, RAMON CESAR;
DE CASTRO, RAQUEL VASCONCELOS GUIMARAES;
DE SOUZA, ALINE FERNANDA;
BRIDI, ALESSANDRA;
LIMA, MARINA AMARO;
FANTINATO NETO, PAULO;
PESSOA, LAIS VICARI DE FIGUEIREDO;
MARTINS, SIMONE MARIA MASSAMI KITAMURA;
DE ANDRADE, ANDRE FURUGEN CESAR;
FREUDE, KRISTINE KARLA;
BRESSAN, FABIANA FERNANDES.
Generation of neural progenitor cells from porcine-induced pluripotent stem cells.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE,
v. 14,
n. 12
NOV 2020.
Web of Science Citations: 0.
BRESSAN, FABIANA FERNANDES;
BASSANEZZE, VINICIUS;
DE FIGUEIREDO PESSOA, LAIS VICARI;
SACRAMENTO, CHESTER BITTENCOURT;
MALTA, TATHIANE MAISTRO;
KASHIMA, SIMONE;
FANTINATO NETO, PAULO;
STREFEZZI, RICARDO DE FRANCISCO;
GODOY PIERI, NAIRA CAROLINE;
KRIEGER, JOSE EDUARDO;
COVAS, DIMAS TADEU;
MEIRELLES, FLAVIO VIEIRA.
Generation of induced pluripotent stem cells from large domestic animals.
STEM CELL RESEARCH & THERAPY,
v. 11,
n. 1
JUN 25 2020.
Web of Science Citations: 0.
SANGUINET, EDUARDO DE O.;
SIQUEIRA, NATALY M.;
MENEZES, FELIPE DE C.;
RASIA, GISELE M.;
LOTHHAMMER, NIVIA;
SOARES, ROSANE M. D.;
MEIRELLES, FLAVIO V.;
BRESSAN, FABIANA F.;
BOS-MIKICH, ADRIANA.
Interaction of fibroblasts and induced pluripotent stem cells with poly(vinyl alcohol)-based hydrogel substrates.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS,
JUN 2019.
Web of Science Citations: 0.
DE FIGUEIREDO PESSOA, LAIS VICARI;
LISBOA PIRES, PEDRO RATTO;
DEL COLLADO, MAITE;
GODOY PIERI, NAIRA CAROLINE;
RECCHIA, KAIANA;
SOUZA, ALINE FERNANDA;
PERECIN, FELIPE;
DA SILVEIRA, JULIANO COELHO;
CESAR DE ANDRADE, ANDRE FURUGEN;
AMBROSIO, CARLOS EDUARDO;
BRESSAN, FABIANA FERNANDES;
MEIRELLES, FLAVIO VIEIRA.
Generation and miRNA Characterization of Equine Induced Pluripotent Stem Cells Derived from Fetal and Adult Multipotent Tissues.
STEM CELLS INTERNATIONAL,
2019.
Web of Science Citations: 1.
DE CASTRO, RAQUEL V. G.;
TAVARES, MARIANA R.;
BRESSAN, FABIANA F.;
PIERI, NAIRA C. G.;
TRINDADE HILL, AMANDA BARACHO;
SOUZA, ALINE F.;
CRUZ, NATHAN DA R. N.;
MARTINS, DANIELE S.;
AMBROSIO, CARLOS E.;
MEIRELLES, FLAVIO V.;
GARCIA, JOAQUIM M.
In vitro identification of a stem cell population from canine hair follicle bulge region.
TISSUE & CELL,
v. 50,
p. 43-50,
FEB 2018.
Web of Science Citations: 1.
GODOY PIERI, NAIRA CAROLINE;
DE SOUZA, ALINE FERNANDA;
BOTIGELLI, RAMON CESAR;
MACHADO, LUCAS SIMOES;
AMBROSIO, CARLOS EDUARDO;
MARTINS, DANIELE DOS SANTOS;
CESAR DE ANDRADE, ANDRE FURUGEN;
MEIRELLES, FLAVIO VIEIRA;
HYTTEL, POUL;
BRESSAN, FABIANA FERNANDES.
Stem cells on regenerative and reproductive science in domestic animals.
VETERINARY RESEARCH COMMUNICATIONS,
v. 43,
n. 1,
p. 7-16,
FEB 2018.
Web of Science Citations: 2.