Role of clusterin/ApoJ on insulin signalling in response to physical exercise in rodents and humans

• Grant number: 15/07199-2
• Support type: Research Grants - Young Investigators Grants
• Duration: October 01, 2016 - September 30, 2020
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Leandro Pereira de Moura
• Grantee: Leandro Pereira de Moura
• Home Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated grant(s): 18/04932-9 - Meta-analysis and physical activity patterns and weight changes in premenopausal women: findings and methods, AV.EXT
• Associated scholarship(s): 18/06833-8 - Could physical exercise improve visual acuity? Effects of different physical training protocols on tear composition and clusterin/ApoJ metabolism in the retina of obese rodent, BP.MS; 16/24406-4 - The role of physical exercise in pathways signaling of clusterin/leptin in hypothalamus of obese mice, BP.DD; 16/14388-9 - Comparison of two exercise protocols on action mechanism of clusterin/ApoJ in lean and obese mice, BP.DR; 16/12569-6 - Effects of different exercise intensities on clusterin metabolism and its interference on insulin signaling in rodents, BP.DD

Abstract

Insulin is an anabolic hormone with fundamental actions in organs and tissues to maintain body homeostasis. Abnormalities in insulin action to stimulate glucose uptake in skeletal muscle and inhibit hepatic glucose production, are usually found in individuals with insulin resistance and antecedent the development of type 2 diabetes mellitus (DM2). The hypertrophied adipose tissue releases several biomolecules such as hormones and pro-inflammatory cytokines and other factors that are involved in the induction of insulin resistance in obese. In this context, the knowledge in molecular biology with other fields of study have been trying to guide which factors are most important in the prevention and treatment of DM2. Clusterin, or apolipoprotein J (ApoJ) via its membrane receptor (LRP2) acts in the insulin signaling potentiating its effects on peripheral tissues (skeletal muscle, liver and adipose) and also in the hypothalamus. However, the expression of this protein in peripheral tissues and its mechanisms of action are not fully understood. Physical exercise is also able to regulate crucial proteins involved in insulin signaling and promote metabolic improvements in animal and human diabetics. However, its effects in the regulation of clusterin and consequently in glucose homeostasis have not been elucidated so far. Thus, understanding the molecular mechanisms involved in the action of clusterin in insulin signaling in the liver and muscle tissues in the exercise condition becomes essential for discovery of new therapeutic mechanisms, as well as for more effective prescription of physical exercise and safe for individuals affected by obesity and DM2. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEREIRA, RODRIGO MARTINS; DA CRUZ RODRIGUES, KELLEN CRISTINA; ANARUMA, CHADI PELLEGRINI; SANT'ANA, MARCELLA RAMOS; PEREIRA DE CAMPOS, THAIS DANTIS; GASPAR, RODRIGO STELIZER; CANCIGLIERI, RAPHAEL DOS SANTOS; DE MELO, DIEGO GOMES; MEKARY, RANIA A.; RAMOS DA SILVA, ADELINO SANCHEZ; CINTRA, DENNYS ESPER; ROPELLE, EDUARDO ROCHETE; PAULI, JOSE RODRIGO; DE MOURA, LEANDRO PEREIRA. Short-term strength training reduces gluconeogenesis and NAFLD in obese mice. Journal of Endocrinology, v. 241, n. 1, p. 59-70, APR 2019. Web of Science Citations: 1.

DA CRUZ RODRIGUES, KELLEN C.; PEREIRA, RODRIGO M.; DE CAMPOS, THAIS D. P.; DE MOURA, RODRIGO F.; DA SILVA, ADELINO S. R.; CINTRA, DENNYS E.; ROPELLE, EDUARDO R.; PAULI, JOSE R.; DE ARAUJO, MICHEL B.; DE MOURA, LEANDRO P. The Role of Physical Exercise to Improve the Browning of White Adipose Tissue via POMC Neurons. FRONTIERS IN CELLULAR NEUROSCIENCE, v. 12, MAR 28 2018. Web of Science Citations: 9.

PEREIRA, RODRIGO MARTINS; MEKARY, RANIA A.; DA CRUZ RODRIGUES, KELLEN CRISTINA; ANARUMA, CHADI PELLEGRINI; ROPELLE, EDUARDO ROCHETE; RAMOS DA SILVA, ADELINO SANCHEZ; CINTRA, DENNYS ESPER; PAULI, JOSE RODRIGO; DE MOURA, LEANDRO PEREIRA. Protective molecular mechanisms of clusterin against apoptosis in cardiomyocytes. HEART FAILURE REVIEWS, v. 23, n. 1, p. 123-129, JAN 2018. Web of Science Citations: 6.

PEREIRA, RODRIGO MARTINS; BOTEZELLI, JOSE DIEGO; DA CRUZ RODRIGUES, KELLEN CRISTINA; MEKARY, RANIA A.; CINTRA, DENNYS ESPER; PAULI, JOSE RODRIGO; RAMOS DA SILVA, ADELINO SANCHEZ; ROPELLE, EDUARDO ROCHETE; DE MOURA, LEANDRO PEREIRA. Fructose Consumption in the Development of Obesity and the Effects of Different Protocols of Physical Exercise on the Hepatic Metabolism. NUTRIENTS, v. 9, n. 4 APR 2017. Web of Science Citations: 17.