Postoperative pain: gene expression of pain-related purinergic receptors in the dorsal root ganglia in a model of sole incision in rats.

• Grant number: 15/13917-5
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2016
• End date: December 31, 2018
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Hazem Adel Ashmawi
• Grantee: Hazem Adel Ashmawi
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Luiz Fernando Onuchic

Abstract

Acute Postoperative pain of moderate and severe intensities is still highly prevalent, despite available methods for their treatment. One of the probable causes are the incomplete knowledge of the mechanisms responsible for the genesis and maintenance of postoperative pain. Treatment of postoperative pain decreases postoperative morbidities and costs of treatment. Purinergic receptors are involved in transduction of nociceptive stimuli and central sensitization . The role of these receptors is not yet established in postoperative pain pathophysiology. The aim of this study is to evaluate genic and proteic expression of purinergic receptors of the P2X group - P2x1, P2X2, P2X3, P2X4, P2X5, P2X6, P2X7 in dorsal root ganglia in the plantar incision model in rat paw . Expression will be obtained in the following time points: before incision, 12 hours after incision, 1st, 2nd, 5th and 10th postoperative days. Behavioral tests will be performed - evoked pain - heat hyperalgesia (Hargreaves test), mechanical hyperalgesia (electronic Von Frey) and spontaneous pain (guarding pain). (AU)