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An integrated approach to explore a novel paradigm for biofuel production from lignocellulosic feedstocks

Grant number: 15/50612-8
Support type:Research Projects - Thematic Grants
Duration: November 01, 2016 - October 31, 2021
Field of knowledge:Engineering - Chemical Engineering
Cooperation agreement: BBSRC, UKRI
Principal Investigator:Telma Teixeira Franco
Grantee:Telma Teixeira Franco
Principal investigator abroad: David Leak
Institution abroad: University of Bath, England
Home Institution: Núcleo Interdisciplinar de Planejamento Energético (NIPE). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Co-Principal Investigators:Adilson Roberto Gonçalves ; André Ricardo de Lima Damasio ; Carla Kazue Nakao Cavaliero ; José Maria Ferreira Jardim da Silveira ; Marcelo Mendes Brandao ; Sarita Candida Rabelo
Associated grant(s):17/19157-8 - BBEST 2017 - Brazilian bioenergy Science and Technology conference, AR.BR
17/10367-0 - 4th European Congress of Applied Biotechnology, AR.EXT
Associated scholarship(s):19/13946-6 - An integrated approach to explore a novel paradigm for biofuel production from lignocellulosic feedstocks, BP.TT
19/03200-7 - Development of a fixed-structured bed anaerobic bioreactor with assisted electron transfer for biogas production and wastewater treatment, BP.DR
17/24503-2 - Use of agroindustrial lignocellulosic waste for the production of oligosaccharides aiming at obtaining a prebiotic agent in human health, BP.DR
+ associated scholarships 18/20173-0 - Technical and environmental assessment of sugarcane straw and eucalyptus forest residues as feedstocks for biorefineries at São Paulo State, BP.DD
18/09893-1 - Biogas production integrated to the concept of lignocellulosic biomass: operational aspects and use of nanoparticles, BP.DR
18/01759-4 - Metabolic engineering of Saccharomyces cerevisiae to produce second generation ethanol from xylooligosaccharides, BP.DR
17/16159-0 - Optimization of cello-oligosaccharides production by enzymatic hydrolysis of sugarcane straw via heterologous expression of cellulolytic and oxidative enzymes, BP.DD
17/15477-8 - Development of a low severity pretreatment process for the production of sugarcane straw oligosaccharides, BP.PD
17/10083-1 - Genetic engineering of a fungal platform to secrete lignocellulolytic enzymes for oligosaccharide production, BP.PD - associated scholarships

Abstract

In this project we intend to demonstrate that it is more sensible (logical and economic) not to pre-treat lignocellulose so harshly, and have a more "holistic" approach to the process delivering the desired products whilst minimising overall process energy and cost by working on the optimisation of generating partial breakdown products and ensuring that the subsequent fermentation organism is able to convert these directly to product. The most commonly employed class of fermentation organisms - yeasts - will be engineered to be able to convert the oligomeric sugars directly. However, there is a class of organisms -Geobacillus -that have been quite extensively studied by one of the UK groups, which already naturally has the propensity to utilise oligomeric sugars and can also be readily engineered to optimise key metabolic pathways. Therefore, in this project we will use a representative of this group of bacteria to compare performance with the engineered yeast. We also propose to consider three different lignocellulosic feedstocks in this study, all of which have the potential to be used for sustainable fuels and chemicals production: Brazilian cane straw - which is current left in the fields after harvesting, Miscanthus - which is grown in the UK for burning in power stations (co-firing) and has a lot of similarities to cane straw, and Eucalyptus forestry residues, which are abundant in Brazil and represent a different type of opportunity and material to evaluate. Some of the team involved will focus on developing methods to convert these to oligosaccharides that can be taken up by these new strains. This will be a combination of less severe (than currently) pre-treatment and the use of selected enzymes to produce the oligo-saccharides required. Another part of the team will focus on producing the enzymes required for these conversions to oligosaccharides, while a third group will engineer the yeast strains to use oligosaccharides of both xylose and glucose. To increase the energy efficiency of the feedstocks in the new lignocelulose mills we are going to recover chemicals and biogas from the liquid effluents, vinasse and hemicellulose hydrolysates, by integrating anaerobic digestion (AD) to the process. AD with mixed culture fermentation will improve the energy ratio bringing biogas production and fertilizers as products. Underpinning all this is the need to ensure that the outputs of this work remains relevant to the industry processes that they potentially feed into. Therefore, we have a team of LCA experts ensuring that feedstock/ product choice is appropriate, that the proposed process optimisation approaches are delivering a positive impact on process performance and pinpointing where further changes/ modifications could be made to further improve matters. (AU)

Articles published in Agência FAPESP Newsletter about the research grant
Joint FAPESP-BBSRC Collaborative Project Meeting 
Articles published in other media outlets (1 total):
Mapeamento de biomassa residual de eucalipto da fase agrícola na região administrativa de Campinas-SP 

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FERRARI, FELIPE AUGUSTO; BRANDAO PEREIRA, JORGE FERNANDO; WITKAMP, GEERT-JAN; SOARES FORTE, MARCUS BRUNO. Which Variables Matter for Process Design and Scale-Up? A Study of Sugar Cane Straw Pretreatment Using Low-Cost and Easily Synthesizable Ionic Liquids. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 7, n. 15, p. 12779-12788, AUG 5 2019. Web of Science Citations: 0.
AZAMBUJA, SUEELLEN P. H.; TEIXEIRA, GLEIDSON S.; ANDRIETTA, MARIA G. S.; TORRES-MAYANGA, PAULO C.; FORSTER-CARNEIRO, TANIA; ROSA, CARLOS A.; GOLDBECK, ROSANA. Analysis of metabolite profiles of Saccharomyces cerevisiae strains suitable for butanol production. FEMS Microbiology Letters, v. 366, n. 13 JUL 2019. Web of Science Citations: 0.
SODRE, VICTORIA; ARAUJO, JUSCEMACIA NASCIMENTO; GONCALVES, THIAGO AUGUSTO; VILELA, NATHALIA; KIMUS BRAZ, ANTONIO SERGIO; FRANCO, TELMA TEIXEIRA; NETO, MARIO DE OLIVEIRA; DE LIMA DAMASIO, ANDRE RICARDO; GARCIA, WANIUS; SQUINA, FABIO MARCIO. An alkaline active feruloyl-CoA synthetase from soil metagenome as a potential key enzyme for lignin valorization strategies. PLoS One, v. 14, n. 2 FEB 25 2019. Web of Science Citations: 0.
CRIVELENTE HORTA, MARIA AUGUSTA; FERREIRA FILHO, JAIRE ALVES; MURAD, NATALIA FARAJ; SANTOS, EIDY DE OLIVEIRA; DOS SANTOS, CLELTON APARECIDO; MENDES, JULIANO SALES; BRANDAO, MARCELO MENDES; AZZONI, SINDELIA FREITAS; DE SOUZA, ANETE PEREIRA. Network of proteins, enzymes and genes linked to biomass degradation shared by Trichoderma species. SCIENTIFIC REPORTS, v. 8, JAN 22 2018. Web of Science Citations: 5.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.