Microbiomes and immunobiological aspects in endodontic infections

• Grant number: 15/23479-5
• Support Opportunities: Research Projects - Thematic Grants
• Duration: November 01, 2016 - October 31, 2023
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Brenda Paula Figueiredo de Almeida Gomes
• Grantee: Brenda Paula Figueiredo de Almeida Gomes
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Associated researchers: Bruce J. Paster ; Magda Feres Figueiredo ; Tsute Chen
• Associated scholarship(s): 21/14570-0 - INFLUENCE OF THE INFECTION TIME ON THE MICROBIOLOGICAL (BACTERIA AND FUNGI), CYTOTOXIC AND INFLAMMATORY PROFILE OF THE MICROBIAL COMMUNITY OF SECONDARY/PERSISTENT ENDODONTIC INFECTIONS, BP.DR; 17/25090-3 - Microbiological and fadA family analysis in root canals and periapical lesions of teeth with secondary infection and their relation with the levels of proinflammatory cytokines and MMPs, BP.PD; 19/09115-1 - Effect of chemomechanical preparation associated with photodynamic therapy in the reduction of microbial, LPS, LTA, substance p and pro-inflammatory cytokines in primary endodontic infection, BP.MS; + associated scholarships 19/19300-0 - Investigation of the clinical, microbiological and immunological aspects of teeth with diagnosis of primary periodontal lesions with secondary endodontic involvement, BP.DR; 19/14448-0 - Microbiomes and immunobiological aspects in endodontic infections, BP.PD; 17/25242-8 - Clinical, microbiological and immunological monitoring of patients undertaken to endodontic therapy with diagnosis of irreversible Pulpitis, BP.DR; 17/26973-6 - Analysis of biofilm formation capacity and virulence factors of strains of Enterococcus faecalis isolated from teeth associated with failure of endodontic treatment, BP.IC; 18/00587-5 - Investigation of virulence genes of Enterococcus faecalis isolated from teeth associated with failure of endodontic treatment, BP.IC; 17/18838-1 - Characterization of the microbiological and endotoxic profile of patients with chronic periodontitis and secondary endodontic involvement submitted to the endodontic therapy, BP.MS; 17/16516-7 - Investigation of the microbiological content, matrix metalloproteinase expression and antimicrobial susceptibility of specific bacteria in endodontic failure cases, BP.DR; 17/18459-0 - Evaluation of the infectious/endotoxic content of root canals of teeth with symptomatic/asymptomatic endodontic infections, BP.MS; 16/23950-2 - Monitoring of infectious contents, endotoxic and gene presence of resistance to antibiotics of symptomatic and asymptomatic primary infections during endodontic therapy, BP.DR - associated scholarships

Abstract

Over 15 million endodontic treatments (ET) are performed annually in the US and also in Brazil due to endodontic infections (EI). Such infections can cause signs and symptoms ranging from a mild discomfort to severe life-threatening infections, such as cellulitis, stroke or even death. This study aims to investigate the microbiomes present in different types of EI, and the levels of LPS, LTA, MMP, substance P and inflammatory cytokines in the different clinical situations in order to evaluate the effect of the ET in the reduction/elimination of the microbial/endotoxic contents from the root canals. The data achieved will be correlated with clinical and radiographic findings presented by the patients. This study also aims to investigate the susceptibility of selected bacteria to the most commonly used antibiotics in order to provide the current pattern of susceptibility. Molecular methods such as Next Generation Sequencing will be used to assess the biodiversity of the microbiomes; ELISA /cytometry to quantitate the virulence factors and the immunobiological features; and E-test and production of beta-lactamase to obtain a profile of the bacterial susceptibility to various antimicrobial agents. This work is innovative as it will evaluate with high-tech methods the microbiomes, virulence factors and the immunobiological aspects present in different types of EI; correlating them with the clinical and radiographic findings presented by the patients. It will also monitor the bacterial susceptibility to different antibiotics. The results achieved will serve as a baseline for the development of more effective ET. (AU)

Scientific publications

(34)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DUQUE, THAIS M.; PRADO, MAIRA; HERRERA, DANIEL R.; GOMES, BRENDA P. F. A.. Periodontal and endodontic infectious/inflammatory profile in primary periodontal lesions with secondary endodontic involvement after a calcium hydroxide-based intracanal medication. CLINICAL ORAL INVESTIGATIONS, v. 23, n. 1, p. 53-63, JAN 2019. (15/23479-5)

BARBOSA-RIBEIRO, MARLOS; ARRUDA-VASCONCELOS, RODRIGO; DE-JESUS-SOARES, ADRIANA; ZAIA, ALEXANDRE AUGUSTO; RANDI FERRAZ, CAIO CEZAR; AFFONSO DE ALMEIDA, JOSE FLAVIO; GOMES, BRENDA P. F. A.. Effectiveness of calcium hydroxide-based intracanal medication on infectious/inflammatory contents in teeth with post-treatment apical periodontitis. CLINICAL ORAL INVESTIGATIONS, v. 23, n. 6, p. 2759-2766, JUN 2019. (15/23479-5)

FIGUEIREDO DE ALMEIDA GOMES, BRENDA PAULA; HERRERA, DANIEL RODRIGO. Etiologic role of root canal infection in apical periodontitis and its relationship with clinical symptomatology. Brazilian Oral Research, v. 32, n. 1, p. 82-110, 2018. (15/23479-5)

MUNIZ, FRANCISCO WILKER M. G.; MONTAGNER, FRANCISCO; JACINTO, ROGERIO C.; ROSING, CASSIANO K.; GOMES, BRENDA P. F. A.. Correlation between crestal alveolar bone loss with intracanal bacteria and apical lesion area in necrotic teeth. ARCHIVES OF ORAL BIOLOGY, v. 95, p. 1-6, NOV 2018. (15/23479-5)

MARIA CRISTINA CARVALHO; MARIO LUIS ZUOLO; RODRIGO ARRUDA-VASCONCELOS; ARIANE CÁSSIA SALUSTIANO MARINHO; LIDIANE MENDES LOUZADA; PRISCILA AMANDA FRANCISCO; VANESSA GALEGO ARIAS PECORARI; BRENDA PAULA FIGUEIREDO DE ALMEIDA GOMES. Effectiveness of XP-Endo Finisher in the reduction of bacterial load in oval-shaped root canals. Brazilian Oral Research, v. 33, 2019-05-16. (15/23479-5)

BRONZATO, JULIANA D.; BOMFIM, RAFAEL A.; HAYASIDA, GIOVANNA Z. P.; CURI, MARIANA; ESTRELA, CARLOS; PASTER, BRUCE J.; GOMES, BRENDA P. F. A.. Analysis of microorganisms in periapical lesions: A systematic review and meta-analysis. ARCHIVES OF ORAL BIOLOGY, v. 124, APR 2021. (15/23479-5)

BARBOSA-RIBEIRO, MARLOS; ARRUDA-VASCONCELOS, RODRIGO; LOUZADA, LIDIANE M.; LIMA, AUGUSTO R.; MARCIANO, MARINA A.; ALMEIDA, JOSE FLAVIO A.; DE-JESUS-SOARES, ADRIANA; ZAIA, ALEXANDRE A.; FERRAZ, CAIO CEZAR R.; GOMES, BRENDA P. F. A.. Microbiological Investigation in Teeth with Persistent/Secondary Endodontic Infection in Different Stages of Root Canal Retreatment. EUROPEAN ENDODONTIC JOURNAL, v. 5, n. 3, p. 219-225, 2020. (17/25242-8, 19/19300-0, 19/10755-5, 12/23697-4, 15/23479-5)

LIMA, AUGUSTO R.; GANGULY, TRIDIB; WALKER, ALEJANDRO R.; ACOSTA, NATALIA; FRANCISCO, PRISCILA A.; PILEGGI, ROBERTA; LEMOS, JOSE A.; GOMES, BRENDA P. F. A.; ABRANCHES, JACQUELINE. Phenotypic and Genotypic Characterization of Streptococcus mutans Strains Isolated from Endodontic Infections. JOURNAL OF ENDODONTICS, v. 46, n. 12, p. 1876-1883, DEC 2020. (15/23479-5, 16/23950-2, 18/09270-4)

ARRUDA-VASCONCELOS, R.; LOUZADA, L. M.; FERES, M.; TOMSON, P. L.; COOPER, P. R.; GOMES, B. P. F. A.. Investigation of microbial profile, levels of endotoxin and lipoteichoic acid in teeth with symptomatic irreversible pulpitis: a clinical study. International Endodontic Journal, v. 54, n. 1, p. 46-60, JAN 2021. (15/23479-5, 19/10755-5, 17/25242-8, 19/19300-0)

BRONZATO, J. D.; DAVIDIAN, M. E. S.; DE CASTRO, M.; DE-JESUS-SOARES, A.; FERRAZ, C. C. R.; ALMEIDA, J. F. A.; MARCIANO, M. A.; GOMES, B. P. F. A.. Bacteria and virulence factors in periapical lesions associated with teeth following primary and secondary root canal treatment. International Endodontic Journal, v. 54, n. 5, DEC 2020. (15/23479-5)

VELOSO, DEJANILDO J.; ABRAO, FARIZA; MARTINS, CARLOS H. G.; BRONZATO, JULIANA D.; GOMES, BRENDA P. F. A.; HIGINO, JANE S.; SAMPAIO, FABIO C.. Potential antibacterial and anti-halitosis activity of medicinal plants against oral bacteria. ARCHIVES OF ORAL BIOLOGY, v. 110, FEB 2020. (15/23479-5)

BRONZATO, JULIANA D.; BOMFIM, RAFAEL A.; EDWARDS, DAVID H.; CROUCH, DOROTHY; HECTOR, MARK P.; GOMES, BRENDA P. F. A.. Detection of Fusobacterium in oral and head and neck cancer samples: A systematic review and meta-analysis. ARCHIVES OF ORAL BIOLOGY, v. 112, APR 2020. (15/23479-5)

BARBOSA-RIBEIRO, MARLOS; ARRUDA-VASCONCELOS, RODRIGO; LOUZADA, LIDIANE M.; DOS SANTOS, DANIELLE G.; ANDREOTE, FERNANDO D.; GOMES, BRENDA P. F. A.. Microbiological analysis of endodontically treated teeth with apical periodontitis before and after endodontic retreatment. CLINICAL ORAL INVESTIGATIONS, v. 25, n. 4, AUG 2020. (17/25242-8, 15/23479-5, 19/10755-5, 19/19300-0)

LOPES, ERICA M.; PASSINI, MAICON R. Z.; KISHI, LUCIANO T.; CHEN, TSUTE; PASTER, BRUCE J.; GOMES, BRENDA P. F. A.. Interrelationship between the Microbial Communities of the Root Canals and Periodontal Pockets in Combined Endodontic-Periodontal Diseases. MICROORGANISMS, v. 9, n. 9, SEP 2021. (15/23479-5, 19/14448-0)

FRANCISCO, PRISCILA AMANDA; FAGUNDES, PEDRO IVO DA GRACA; LEMES-JUNIOR, JOAO CARLOS; LIMA, AUGUSTO RODRIGUES; PASSINI, MAICON RICARDO ZIEBERG; GOMES, BRENDA P. F. A.. Pathogenic potential of Enterococcus faecalis strains isolated from root canals after unsuccessful endodontic treatment. CLINICAL ORAL INVESTIGATIONS, v. 25, n. 9, p. 5171-5179, SEP 2021. (15/23479-5, 18/00587-5, 17/26973-6)

ARRUDA-VASCONCELOS, RODRIGO; BARBOSA-RIBEIRO, MARLOS; LOUZADA, LIDIANE M.; LEMOS, BEATRIZ I. N.; DE-JESUS-SOARES, ADRIANA; FERRAZ, CAIO C. R.; ALMEIDA, JOSE F. A.; MARCIANO, MARINA A.; GOMES, BRENDA P. F. A.. fficacy of 6% Sodium Hypochlorite on Infectious Content of Teeth with Symptomatic Irreversible Pulpiti. JOURNAL OF ENDODONTICS, v. 48, n. 2, p. 179-189, FEB 2022. (17/25242-8, 15/23479-5, 19/10755-5, 19/19300-0)

GABRIELLI, EZEQUIEL S.; LIMA, AUGUSTO R.; FRANCISCO, PRISCILA A.; HERRERA, DANIEL R.; DE-JESUS-SOARES, ADRIANA; FERRAZ, CAIO C. R.; ALMEIDA, JOSE FLAVIO A.; MARCIANO, MARINA A.; GOMES, BRENDA P. F. A.. Comparative analysis of bacterial content, levels of lipopolysaccharides and lipoteichoic acid in symptomatic and asymptomatic endodontic infections at different stages of endodontic treatment. CLINICAL ORAL INVESTIGATIONS, JUN 2021. (17/18459-0, 15/23479-5)

BRENDA PAULA FIGUEIREDO DE ALMEIDA GOMES; RODRIGO ARRUDA-VASCONCELOS; LIDIANE MENDES LOUZADA; REBECCA FIGUEIREDO DE ALMEIDA-GOMES; ADRIANA DE-JESUS-SOARES; LUCIA RACHEL FIGUEIREDO DE ALMEIDA; EVANDRO ROBERTO BALDACCI. SARS-CoV-2: A Professional and social gamechanger - Medical and dental aspects. Brazilian Dental Journal, v. 32, n. 5, p. 41-54, 2021-12-06. (17/25242-8, 15/23479-5, 19/19300-0)

BRENDA PAULA FIGUEIREDO DE ALMEIDA GOMES; DANIEL RODRIGO HERRERA. Etiologic role of root canal infection in apical periodontitis and its relationship with clinical symptomatology. Brazilian Oral Research, v. 32, 2018-10-18. (15/23479-5)

RODRIGO ARRUDA-VASCONCELOS; LETÍCIA GOMES FERREIRA CHANTRE; ROSANGELA SABBATINI CAPELLA LOPES; CLÁUDIO CERQUEIRA LOPES; MARLOS BARBOSA-RIBEIRO; BRENDA PAULA FIGUEIREDO DE ALMEIDA GOMES. Aplicação do luminol forense para detecção de sangue em limas endodônticas. Rev. odontol. UNESP, v. 46, n. 4, p. 227-231, 2017-08-00. (15/23479-5)

MARTINHO, FREDERICO C.; LEITE, FABIO R. M.; ARRUDA-VASCONCELOS, RODRIGO; DARVEAU, RICHARD P.; GOMES, BRENDA P. F. A.. Influence of Bacterial Profiles in Cytokine and Clinical Features of Endodontic Disease. JOURNAL OF ENDODONTICS, v. 47, n. 8, p. 1265-1271, AUG 2021. (19/10755-5, 15/23479-5, 17/25242-8)

GOMES, BRENDA P. F. A.; BRONZATO, JULIANA D.; ALMEIDA-GOMES, REBECCA F.; PINHEIRO, ERICKA T.; SOUSA, EZILMARA L. R.; JACINTO, ROGERIO C.. Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods. CLINICAL ORAL INVESTIGATIONS, APR 2021. (15/23479-5)

GADE-NETO, CICERO R.; RODRIGUES, RONALDO R.; LOUZADA, LIDIANE M.; ARRUDA-VASCONCELOS, RODRIGO; TEIXEIRA, FABRICIO B.; VIANA CASARIN, RENATO C.; GOMES, BRENDA P. F. A.. Microbiota of periodontal pockets and root canals in induced experimental periodontal disease in dogs. JOURNAL OF INVESTIGATIVE AND CLINICAL DENTISTRY, v. 10, n. 4, JULY 2019. (15/23479-5)

GOMES, BRENDA P. F. A.; BRONZATO, JULIANA D.; ALMEIDA-GOMES, REBECCA F.; PINHEIRO, ERICKA T.; SOUSA, EZILMARA L. R.; JACINTO, ROGERIO C.. Identification of Fusobacterium nucleatum in primary and secondary endodontic infections and its association with clinical features by using two different methods. CLINICAL ORAL INVESTIGATIONS, v. 25, n. 11, p. 6249-6258, NOV 2021. (15/23479-5)

CIMARA B. B. SILVA; JULIANA D. BRONZATO; DANIEL R. HERRERA; FRANCISCO MONTAGNER; ELIFAS L. NUNES; BRENDA P F A GOMES. Efficiency of a digital electrofulguration system in contaminated root canals in vitro. Brazilian Dental Journal, v. 32, n. 3, p. 1-9, 2021-11-08. (15/23479-5)

AVEIRO, E.; CHIARELLI-NETO, V. M.; DE-JESUS-SOARES, A.; ZAIA, A. A.; FERRAZ, C. C. R.; ALMEIDA, J. F. A.; MARCIANO, M. A.; FERES, M.; GOMES, B. P. F. A.. Efficacy of reciprocating and ultrasonic activation of 6% sodium hypochlorite in the reduction of microbial content and virulence factors in teeth with primary endodontic infection. International Endodontic Journal, v. 53, n. 5, JAN 2020. (15/23479-5)

NEELAKANTAN, P.; HERRERA, D. R.; PECORARI, V. G. A.; GOMES, B. P. F. A.. Endotoxin levels after chemomechanical preparation of root canals with sodium hypochlorite or chlorhexidine: a systematic review of clinical trials and meta-analysis. International Endodontic Journal, v. 52, n. 1, p. 19-27, JAN 2019. (15/23479-5)

ALVES-SILVA, ESDRAS GABRIEL; ARRUDA-VASCONCELOS, RODRIGO; LOUZADA, LIDIANE MENDES; DE-JESUS-SOARES, ADRIANA; FERRAZ, CAIO CEZAR RANDI; ALMEIDA, JOSE FLAVIO AFFONSO; MARCIANO, MARINA ANGELICA; STEINER-OLIVEIRA, CAROLINA; BELLO-SILVA, MARINA STELLA; SHEMESH, HAGAY; et al. The effect of photodynamic therapy on postoperative pain in teeth with primary endodontic infection. Photodiagnosis and Photodynamic Therapy, v. 37, MAR 2022. (19/19300-0, 17/25242-8, 19/10755-5, 15/23479-5, 19/09115-1)

FRANCISCO, PRISCILA AMANDA; FAGUNDES, PEDRO IVO DA GRACA; LEMES-JUNIOR, JOAO CARLOS; LIMA, AUGUSTO RODRIGUES; PASSINI, MAICON RICARDO ZIEBERG; GOMES, BRENDA P. F. A.. Pathogenic potential of Enterococcus faecalis strains isolated from root canals after unsuccessful endodontic treatment. CLINICAL ORAL INVESTIGATIONS, FEB 2021. (17/26973-6, 15/23479-5, 18/00587-5)

GOMES, BRENDA P. F. A.; LOUZADA, LIDIANE M.; ALMEIDA-GOMES, REBECCA F.; PINHEIRO, ERICKA T.; SOUSA, EZILMARA L. R.; JACINTO, ROGERIO C.; ARRUDA-VASCONCELOS, RODRIGO. Investigation of Filifactor alocis in primary and in secondary endodontic infections: A molecular study. ARCHIVES OF ORAL BIOLOGY, v. 118, OCT 2020. (19/19300-0, 17/25242-8, 15/23479-5)

GOMES, BRENDA P. F. A.; BERBER, VANESSA B.; MARINHO, ARIANE C. S.; LOUZADA, LIDIANE M.; ARRUDA-VASCONCELOS, RODRIGO; PASSINI, MAICON R. Z.; LOPES, ERICA M.; PECORARI, VANESSA G. A.; CHEN, TSUTE; PASTER, BRUCE J.. Chemomechanical preparation influences the microbial community and the levels of LPS, LTA and cytokines in combined endodontic-periodontal lesions: A clinical study. JOURNAL OF PERIODONTAL RESEARCH, DEC 2021. (17/25242-8, 14/01655-3, 19/10755-5, 15/23479-5, 19/19300-0)

FRANCISCO, P. A.; DELBONI, M. G.; LIMA, A. R.; XIAO, Y.; SIQUEIRA, W. L.; GOMES, B. P. F. A.. Proteomic profile of root canal contents in teeth with post-treatment endodontic disease. International Endodontic Journal, v. 52, n. 4, p. 451-460, APR 2019. (17/16516-7, 15/23479-5, 16/19743-1, 15/19215-2)

MARINHO, A. C. S.; TO, T. T.; DARVEAU, R. P.; GOMES, B. P. F. A.. Detection and function of lipopolysaccharide and its purified lipid A after treatment with auxiliary chemical substances and calcium hydroxide dressings used in root canal treatment. International Endodontic Journal, v. 51, n. 10, p. 1118-1129, OCT 2018. (13/02402-9, 15/23479-5, 13/17928-6)

LOUZADA, LIDIANE M.; ARRUDA-VASCONCELOS, RODRIGO; DUQUE, THAIS M.; CASARIN, V, RENATO C.; FERES, MAGDA; GOMES, BRENDA P. F. A.. Clinical Investigation of Microbial Profile and Levels of Endotoxins and Lipoteichoic Acid at Different Phases of the Endodontic Treatment in Teeth with Vital Pulp and Associated Periodontal Disease. JOURNAL OF ENDODONTICS, v. 46, n. 6, p. 736-747, JUN 2020. (17/18838-1, 15/23479-5)