Effect of Novel Nanotechnology to Conventional Glass Ionomer Cement on its Physical-Chemical-Biological Properties

• Grant number: 16/13786-0
• Support type: Regular Research Grants
• Duration: December 01, 2016 - November 30, 2018
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal researcher: Kamila Rosamilia Kantovitz
• Grantee: Kamila Rosamilia Kantovitz
• Home Institution: Centro de Pesquisas Odontológicas São Leopoldo Mandic. Faculdade São Leopoldo Mandic (SLMANDIC). Sociedade Regional de Ensino e Saúde S/S Ltda (SRES). Campinas , SP, Brazil
• Assoc. researchers: Francisco Humberto Nociti Junior ; Paulo Noronha Lisboa Filho

Abstract

Titanium dioxide (TiO2) has been suggested as a promising material in numerous applications including dental restorative materials. However, the influence of nanotechnology on physic-chemical and biological properties of glass ionomer cement (GIC) is not well established. Thus, this in vitro study investigates the influence of the addition of different concentrations of TiO2 nanotubes in physical-chemical and biological properties of conventional GIC. The powder component of GIC was incorporated with TiO2 nanotubes [3%, 5% and 7% (w/w)], and unblended powder was used as control. Physical-chemical analysis included: 1. Spectroscopy energy dispersive (EDS) and Fourier-transform Raman (FT-Raman) (n=4) - detector X-ray system attached to Scanning Electron Microscopy (SEM) set at 15 kV, working distance of 20 mm and 100X and RFS 100/S, respectively; 2. Superface roughness (SR) (n=8) -surface roughness tester - 1,25 mm in length/cut-off of 0,25 mm; 3. Knoop Hardness (SH) - Knoop microhardness (50 g/10 s); 4. Sorption (Wsp) and solubility (Wsl) - calculation of sorption and water solubility (¼g/mm3); 5. Fluoride releasing analysis - specimens will be immersed in different storage media: RE - remineralization solution; pH=7.0; DE - demineralization solution; pH=4.3) at 37oC for 15 days, and specific electrodes will be used to determine fluoride concentration at 1st, 2nd, 3rd, 5th, 7th, 9th, 12th, 15th days). For biological evaluations, the tests will consist: 1. Cell metabolism by MTT assay in four evaluation times (6, 24, 48 and 72 h); 2. Cell morphology by SEM; 3. Quantification of collagen and non-collagen human fibroblast fibers by Sirius Red staining. The biological experiments will be performed in triplicate for each experimental group. The experiments will be performed in triplicate for each experimental group. Data will be analyzed by appropriate statistical tests, considering a significance level of 5%. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ANA MARA DA SILVA MORAIS; YSADORA MAYARA REALI PEREIRA; ISAAC JORDÃO DE SOUZA-ARAÚJO; DANIEL FURTADO SILVA; VANESSA GALLEGO ARIAS PECORARI; ORISSON PONCE GOMES; FRANCISCO HUMBERTO NOCITI-JÚNIOR; REGINA MARIA PUPPIN-RONTANI; WALDEMIR FRANCISCO VIEIRA-JUNIOR; PAULO NORONHA LISBOA-FILHO; et al. TiO2 nanotube-containing glass ionomer cements display reduced aluminum release rates. Brazilian Oral Research, v. 36, 2022-07-11. (19/14078-8, 16/13786-0)

CIBIM, DANIELA DELLOSSO; SAITO, MIKI TAKETOMI; GIOVANI, PRISCILA ALVES; SANCHES BORGES, ANA FLAVIA; ARIAS PECORARI, VANESSA GALLEGO; GOMES, ORISSON PONCE; LISBOA-FILHO, PAULO NORONHA; NOCITI-JUNIOR, FRANCISCO HUMBERTO; PUPPIN-RONTANI, REGINA MARIA; KANTOVITZ, KAMILA ROSAMILIA. Novel Nanotechnology of TiO2 Improves Physical-Chemical and Biological Properties of Glass Ionomer Cement. INTERNATIONAL JOURNAL OF BIOMATERIALS, 2017. (16/13786-0)

GIOVANI, PRISCILA A.; SALMON, CRISTIANE R.; MARTINS, LUCIAN; LEME, ADRIANA F. P.; PUPPIN-RONTANI, REGINA M.; MOFATTO, LUCIANA S.; NOCITI JR, FRANCISCO H.; KANTOVITZ, KAMILA R.. Membrane proteome characterization of periodontal ligament cell sets from deciduous and permanent teeth. Journal of Periodontology, v. 90, n. 7, p. 775-787, JUL 2019. (16/13786-0, 16/02942-1, 15/06372-2)

KANTOVITZ, KAMILA ROSAMILIA; FERNANDES, FERNANDO PELEGRIM; FEITOSA, ISABELLA VIDAL; LAZZARINI, MARCELA OLIVEIRA; DENUCCI, GIOVANNA CORREA; GOMES, ORISSON PONCE; GIOVANI, PRISCILA ALVES; SILVA MOREIRA, KELLY MARIA; ARIAS PECORARI, VANESSA GALLEGO; SANCHES BORGES, ANA FLAVIA; et al. TiO2 nanotubes improve physico-mechanical properties of glass ionomer cement. Dental Materials, v. 36, n. 3, p. E85-E92, MAR 2020. (16/13786-0, 17/03005-4)

GIOVANI, PRISCILA A.; MARTINS, LUCIANE; SALMON, CRISTIANE R.; MOFATTO, LUCIANA S.; LEME, ADRIANA F. P.; PUPPIN-RONTANI, REGINA M.; KOLLI, TAMARA N.; FOSTER, BRIAN L.; NOCITI, JR., FRANCISCO H.; KANTOVITZ, KAMILA R.. Comparative proteomic analysis of dental cementum from deciduous and permanent teeth. JOURNAL OF PERIODONTAL RESEARCH, v. 56, n. 1, NOV 2020. (16/02942-1, 13/08293-7, 16/13786-0, 19/13242-9)

ISAAC JORDÃO DE SOUZA ARAÚJO; MARIANA GALLANTE RICARDO; ORISSON PONCE GOMES; PRISCILA ALVES GIOVANI; JÚLIA PUPPIN-RONTANI; VANESSA ARIAS PECORARI; ELIZABETH FERREIRA MARTINEZ; MARCELO HENRIQUE NAPIMOGA; FRANCISCO HUMBERTO NOCITI JUNIOR; REGINA MARIA PUPPIN-RONTANI; et al. Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence. Brazilian Oral Research, v. 35, p. -, 2021. (19/14078-8, 16/13786-0)