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Profile of gene expression associated with heat stress in Nellore and Caracu

Abstract

The primary purpose of this proposal is to quantify and compare the effect of thermal challenge on the expression profile of coding genes of the heat shock proteins 60, 70 and 90 (Hsp60, Hsp70 and Hsp90) among Nellore (Bos indicus) and Caracu (Bos taurus) breeds of cattle. During the summer months, when temperatures reach their peak (October-February), the rectal and skin surface temperatures, heart and respiratory rates will be measured in the animals of both races. Animal blood samples will be collected before (in the morning - 7:30 am) and after (the afternoon - 3:00 pm) exposure to the sun, for metering adrenocorticotropic hormone (ATCH) and cortisol. Blood samples will be used for mRNA extraction and study of the levels of transcripts of the coding genes of the heat shock proteins Hsp60, Hsp70 and Hsp90 by real-time PCR. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LIMA, SERGIO B. G. P. N. P.; STAFUZZA, NEDENIA BONVINO; PIRES, BIANCA V.; BONILHA, SARAH F. M.; CYRILLO, JOSLAINE N. S. G.; NEGRAO, JOAO A.; PAZ, CLAUDIA C. P. Effect of high temperature on physiological parameters of Nelore (Bos taurus indicus) and Caracu (Bos taurus taurus) cattle breeds. TROPICAL ANIMAL HEALTH AND PRODUCTION, v. 52, n. 5 MAR 2020. Web of Science Citations: 3.
PIRES, B. V.; STAFUZZA, N. B.; LIMA, S. B. G. P. N. P.; NEGRAO, J. A.; PAZ, C. C. P. Differential expression of heat shock protein genes associated with heat stress in Nelore and Caracu beef cattle. LIVESTOCK SCIENCE, v. 230, DEC 2019. Web of Science Citations: 1.

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