Bases of cellular immune response in chromoblastomycosis and sporotrichosis: implications for vaccine therapy

• Grant number: 16/04729-3
• Support Opportunities: Research Projects - Thematic Grants
• Start date: December 01, 2016
• End date: November 30, 2021
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Sandro Rogerio de Almeida
• Grantee: Sandro Rogerio de Almeida
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Karen Spadari Ferreira
• Associated research grant(s): 17/13722-5 - Nucleation at USP INCT 2016 "Emerging Fungal Infections" - Sporotrichosis nucleus, AV.BR
• Associated scholarship(s): 19/14347-9 - Macrophages and neutrophils' effector function and metabolism over Fonseca pedrosoi infection, BP.PD; 19/06564-0 - Evaluation of cellular immune response in murine chromoblastomycosis after immunization with heat-killed Fonsecaea pedrosoi, BP.IC; 18/11624-9 - Identification of immunogenic peptides of Fonsecaea pedrosoi and its possible use as vaccine in the experimental chromoblastomycosis, BP.PD; 18/07073-7 - Determination of innate and adaptive mechanisms in the recognition of Sporothrix brasiliensis by macrophages and dendritic cells, BP.PD

Abstract

In recent decades, there was observed an increased incidence of fungal infections. This occurred due mainly invasive medical procedures, long periods of hospitalization and increased the number of immunocompromised patients due to infectious diseases such as HIV or treatments that induce immune deficiency, such as immunosuppressive agents and drugs used in the treatment of cancer. In this context, the fungi that cause subcutaneous mycoses, such as Fonseacaea pedrosoi and Sporothrix brasiliensis are gaining prominent clinical importance due to their refractory nature of current therapeutic approaches and the involvement of immunocompromised, in whom the disease is more serious. Much of this framework is due to the little knowledge we have about the pathogenic mechanisms involved in disease, making it difficult to understand them, the development of new therapeutic strategies and detect possible drug targets. Immunological mechanisms of defense against Sporothrix brasiliensis and F. pedrosoi are not well established, but the host immune response plays a decisive role in the evolution of these diseases. Knowing that cell from the innate immune system like; macrophages, neutrophils and dendritic cells are important against various pathogens, including fungi, we propose to elucidate their roles in chromoblastomycosis and sporotrichosis. On the other hand, also arises the interest in mapping and sequencing antigens of the major etiological agents of chromoblastomycosis and sporotrichosis; F. pedrosoi and S. brasiliensis, as from the knowledge of reactive antigens can initiate the studies even achieve an immune treatment for these mycoses. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA XISTO, MARIANA INGRID DUTRA; SANTOS, SUELEN S.; ROSSATO, LUANA; YAMADA YOSHIKAWA, FABIO SEITI; TAVARES HAIDO, ROSA MARIA; DE ALMEIDA, SANDRO ROGERIO; BARRETO-BERGTER, ELIANA. Peptidorhamnomannan from Lomentospora prolificans modulates the inflammatory response in macrophages infected with Candida albicans. BMC Microbiology, v. 20, n. 1, AUG 6 2020. (16/04729-3)

FOGACA DE ALMEIDA, JOSE ROBERTO; JANNUZZI, GRASIELLE PEREIRA; KAIHAMI, GILBERTO HIDEO; DANTAS BREDA, LEANDRO CARVALHO; FERREIRA, KAREN SPADARI; DE ALMEIDA, SANDRO ROGERIO. An immunoproteomic approach revealing peptides from Sporothrix brasiliensis that induce a cellular immune response in subcutaneous sporotrichosis. SCIENTIFIC REPORTS, v. 8, MAR 8 2018. (16/04729-3)