Myxosporean parasites of commercially important Perciformes fish (Cichlaspp. and Plagioscion spp.) in the Amazon Basin

• Grant number: 16/22047-7
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2017
• End date: January 31, 2019
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Inland Water Fishery Resources
• Principal Investigator: Edson Aparecido Adriano
• Grantee: Edson Aparecido Adriano
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil
• Associated scholarship(s): 18/02219-3 - MYXOZOAN of fish from Amazon - molecular biology, morphology, histology and ultrastructure, BP.TT

Abstract

Myxozoans comprise a wide range of endoparasites (recently shown to be highly divergent cnidarians), with around 2,400 species currently known. Some are economically important parasites of fish. There are two major clades of myxozoans (currently referred to as classes), the primitive Malacosporea Canning, Curry, Feist, Longshaw & Okamura, 2000, and the derived Myxosporea Bütschli, 1881. The proposed project is part of an ongoing study of myxosporeans that infect fish economically important, with a focus on systematic, diversity, biology and potential pathogenicity of these parasites in Amazonian. The highly diversified Amazonian ichthyofauna includes fish that belong to the genera Cichla (Perciformes: Cichlidae) and Plagioscion (Perciformes: Sciaenidae) whose members provide important sources of income and food for inhabitants of the region. The genus Cichla includes 15 piscivorous species (popularly known as Tucunaré (in Portuguese) and Peacock bass) that are considered to importantly contribute to the structure and function of aquatic ecosystems in South America. The genus Plagioscion includes five species of sciaenids, four of which occur in the Amazon. These species are particularly important for commercial and subsistence fishing. Their adaptation to the fresh water environment also provides a biogeographic indicator of geological events in the South American region. To characterize myxosporean diversity and effects on hosts the project will conduct morphological and molecular analyses. The latter will involve sequencing of the small subunit ribosomal DNA (SSU-rDNA) (important in species identification) and of the ribosomal intergenic region (ITS-1) (to describe intraspecific genetic variability). Morphology and details of host-parasite interactions will be investigated by examining plasmodia structure, sporogenesis, and host tissue reactions associated with infections using light microscopy, transmission and scanning electron microscopy and confocal laser scanning microscopy. Pathogenicity of parasites will also be evaluated by gross examination of infected fish to identify e.g. swelling, ascites, discoloration, emaciation. Samples of the parasites used in this study will be obtained in field work in areas of the municipalities of Santarém, PA, Manaus, AM and Macapá, AP. Fixation will be performed according to the methodology recommended for each of the aforementioned techniques (10% buffered formalin for morphological analyses; absolute ethanol for molecular analyses; 2.5% glutaraldehyde in 0.1M cacodylate buffer (pH 7.4) for electron microscopy; 4% paraformaldehyde in 0.01 M PBS for microscopy confocal laser scanning). (AU)

Scientific publications

(14)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ZATTI, SUELLEN A.; ATKINSON, STEPHEN D.; MAIA, ANTONIO A. M.; BARTHOLOMEW, JERRI L.; ADRIANO, EDSON A.. Ceratomyxa gracillima n. sp (Cnidaria: Myxosporea) provides evidence of panmixia and ceratomyxid radiation in the Amazon basin. Parasitology, v. 145, n. 9, p. 1137-1146, AUG 2018. (16/22047-7, 13/21374-6)

SILVA, JULIANA M. A.; ADRIANO, EDSON A.; VIEIRA, LISANDRO J.; NALDONI, JULIANA; SANTOS, FRANCISCO G. A.. The Amazonian fish Colossoma macropomum harbors high myxosporean diversity: A description of three novel species from a fish farm. Microbial Pathogenesis, v. 153, APR 2021. (16/22047-7)

DE AGUIAR, JULIO CESAR CENCI; DOMINGUES, MARCUS VINICIUS; SILVA, WILLIAN; CECCARELLI, PAULO SERGIO; ADRIANO, EDSON APARECIDO; SOARES, GEUSIVAM BARBOSA. orphology and molecular phylogeny of Pauciconfibuloides amazonica gen. n. sp. n. (Platyhelminthes, Monogenoidea) parasitizing the Amazonian croaker Plagioscion squamosissimu. Parasitology International, v. 87, APR 2022. (13/21374-6, 16/22047-7, 17/17531-0, 13/20770-5)

ZATTI, SUELLEN A.; ARAUJO, BRUNO L.; ADRIANO, EDSON A.; MAIA, ANTONIO A. M.. A new freshwater Ceratomyxa species (Myxozoa: Ceratomyxidae) parasitizing a sciaenid fish from the Amazon Basin, Brazil. Parasitology International, v. 97, p. 8-pg., 2023-12-01. (16/22047-7, 18/19285-9)

CAPODIFOGLIO, KASSIA R. H.; MEIRA, CAROLINE M.; DA SILVA, MARCIA R. M.; CORREA, LINCOLN L.; ADRIANO, EDSON A.; MAIA, ANTONIO A. M.. Morphology and molecular data of two novel cnidarian myxosporean (Myxobolidae) infecting Piaractus brachypomus from the Amazon basin. Acta Tropica, v. 209, SEP 2020. (16/19149-2, 15/07713-8, 13/21374-6, 16/22047-7)

NALDONI, JULIANA; OKAMURA, BETH; HARTIKAINEN, HANNA; CORREA, LINCOLN L.; ADRIANO, EDSON A.. Broad but restricted detection of malacosporeans in a Neotropical cradle of diversification. Parasitology, v. 148, n. 5, p. 511-518, APR 2021. (15/19463-6, 16/22047-7, 18/01425-9, 14/22700-7)

ZATTI, SUELLEN A.; ATKINSON, STEPHEN D.; MAIA, ANTONIO A. M.; CORREA, LINCOLN L.; BARTHOLOMEW, JERRI L.; ADRIANO, EDSON A.. Novel Myxobolus and Ellipsomyxa species (Cnidaria: Myxozoa) parasiting Brachyplatystoma rousseauxii (Siluriformes: Pimelodidae) in the Amazon basin, Brazil. Parasitology International, v. 67, n. 5, p. 612-621, OCT 2018. (16/22047-7, 13/21374-6)

ZATTI, SUELLEN A.; MAIA, ANTONIO A. M.; ADRIANO, EDSON A.. Growing diversity supports radiation of an Ellipsomyxa lineage into the Amazon freshwater: Description of two novel species parasitizing fish from Tapajos and Amazon rivers. Acta Tropica, v. 211, p. 10-pg., 2020-11-01. (16/22047-7, 18/19285-9, 13/21374-6)

CAPODIFOGLIO, KASSIA R. H.; ADRIANO, EDSON A.; NALDONI, JULIANA; MEIRA, CAROLINE M.; DA SILVA, MARCIA R. M.; MAIA, ANTONIO A. M.. Novel myxosporean species parasitizing an economically important fish from the Amazon basin. Parasitology Research, v. 119, n. 4, MAR 2020. (16/22047-7, 14/22700-7, 13/21374-6, 15/07713-8)

CAPODIFOGLIO, KASSIA R. H.; ADRIANO, EDSON A.; SILVA, MARCIA R. M.; MAIA, ANTONIO A. M.. The resolution of the taxonomic dilemma of Myxobolus colossomatis and description of two novel myxosporeans species of Colossoma macropomum from Amazon basin. Acta Tropica, v. 191, p. 17-23, MAR 2019. (15/07713-8, 13/21374-6, 16/22047-7)

ADRIANO, EDSON A.; ZATTI, SUELLEN A.; OKAMURA, BETH. ow to build single-celled cnidarians with worm-like motility: Lessons from Myxozo. Journal of Anatomy, v. 240, n. 3, OCT 2021. (18/01425-9, 15/19463-6, 19/17427-3, 18/19285-9, 16/22047-7)

ZATTI, SUELLEN A.; ATKINSON, STEPHEN D.; MAIA, ANTONIO A. M.; CORREA, LINCOLN L.; BARTHOLOMEW, JERRI L.; ADRIANO, EDSON A.. Novel Myxobolus and Ellipsomyxa species (Cnidaria: Myxozoa) parasiting Brachyplatystoma rousseauxii (Siluriformes: Pimelodidae) in the Amazon basin, Brazil. Parasitology International, v. 67, n. 5, p. 10-pg., 2018-10-01. (16/22047-7, 13/21374-6)

CORREA, LINCOLN LIMA; SOARES, GEUSIVAM BARBOSA; MUELLER, MARIA ISABEL; ADRIANO, EDSON APARECIDO. First morphological and molecular characterization of the genus Eustrongylides nematode larvae infecting fish in the Brazilian Amazon. BIOLOGIA, v. 78, n. 10, p. 8-pg., 2023-05-08. (17/17531-0, 16/22047-7, 17/16546-3)

ARAUJO, BRUNO L.; ADRIANO, EDSON A.; FRANZOLIN, GUSTAVO N.; ZATTI, SUELLEN A.; NALDONI, JULIANA. A novel Ceratomyxa species (Myxozoa: Cnidaria) infecting an Amazonian catfish. Parasitology International, v. 89, p. 8-pg., 2022-08-01. (16/22047-7)