Drug Discovery against Human Infectious Diseases

• Grant number: 15/26722-8
• Support Opportunities: Research Projects - Thematic Grants
• Start date: March 01, 2017
• End date: October 31, 2024
• Field of knowledge: Biological Sciences - Pharmacology - Biochemical and Molecular Pharmacology
• Principal Investigator: Carsten Wrenger
• Grantee: Carsten Wrenger
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• City of the host institution: São Paulo
• Principal investigators: Alessandro Silva Nascimento
• Associated researchers: Cláudio Romero Farias Marinho ; Helio Alexandre Stefani ; Igor Polikarpov
• Associated research grant(s): 19/00899-0 - Structural Dissection of Nanoparticle-assisted Drug Delivery in Human Infectious Diseases, AP.R SPRINT; 17/07253-2 - EMU 2015/26722-8 DLS Zetasizer microV, AP.EMU; 17/03042-7 - EMU 2015/26722-8 CLARIOstar Microplate Reader, AP.EMU; 17/03047-9 - EMU 2015/26722-8 AVANTI J-30 I BIOSAFE centrifuge including rotor, AP.EMU; 17/04782-4 - EMU 2015/26722-8 AKTA pure gel filtration system, AP.EMU
• Associated scholarship(s): 22/01812-8 - Interference with the Assembly of SARS-CoV-2 virions Targeting E Protein by rational drug design, BP.DR; 21/00124-8 - Analysis of the SUMOylation of Plasmodium falciparum proteins during oxidative stress in erythrocytes with glucose-6-phosphate dehydrogenase deficiency, BP.MS; 20/12277-0 - Drug Discovery against Human Infectious Diseases, BP.PD; + associated scholarships 19/20219-3 - Structural Studies of Enzymes Involved in Vitamin B1 and B6 Synthesis in Plasmodium., BP.PD; 18/21213-6 - Structural Characterization of Vitamin B1 and B6 Synthesis Pathway Enzymes in Plasmodium falciparum and Mycobacterium tuberculosis, BP.DD; 18/10150-3 - Understanding the cross-talk between SUMOylation and oxidative stress in P.falciparum, BP.IC; 18/08820-0 - Nanoparticle-assisted Drug Delivery of Vitamin B6 Pathway Inhibitors in Malaria, BP.DR; 17/26358-0 - Dissection of the Lipoic Acid Metabolism as Druggable Target in Malaria, BP.DR; 18/00562-2 - Exploitation of Vitamin B1 as Pro-Drug in Malaria and MRSA, BP.IC; 17/24901-8 - Structural Studies for Drug Discovery Against Human Infectious Diseases, BP.DD - associated scholarships

Abstract

Infectious diseases are a threat to mankind and currently the causative pathogens are spreading geographically due to globalisation and developing drug resistance. Current resistance to chemotherapeutics is occurring to almost all pathogens and therefore new drug targets and/or novel modi of drug target efficacy is urgently needed. In this grant application the focus will be drawn on suicide drug discovery by exploiting the vitamin B1/B6 biosynthetic pathways of the human pathogens Plasmodium falciparum, the parasite responsible for predominate cases of severe malaria, and the multi-resistant bacteria Staphylococcus aureus MRSA. Compounds will be identified and designed for incorporation into pathogens' metabolism after crystal structure evaluation of the respective vitamin B1/B6 biosynthetic enzymes. Thereby the new compounds are intended to poison the respective vitamin-dependent enzyme. Counter selection will be performed against human cells as well as transgenically modified human cells which are expressing nuclear receptors for xenobiotic sensing as reporter gene chimeras. (AU)

Scientific publications

(57)

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

SRINIVASAN, VASUNDARA; BROGNARO, HEVILA; PRABHU, PRINCE R.; DE SOUZA, EDMARCIA ELISA; GUENTHER, SEBASTIAN; REINKE, PATRICK Y. A.; LANE, THOMAS J.; GINN, HELEN; HAN, HUIJONG; EWERT, WIEBKE; et al. Antiviral activity of natural phenolic compounds in complex at an allosteric site of SARS-CoV-2 papain-like protease. COMMUNICATIONS BIOLOGY, v. 5, n. 1, p. 12-pg., 2022-08-11. (15/26722-8, 19/00899-0, 20/12277-0)

BOSCH, SORAYA S.; LUNEV, SERGEY; BATISTA, FERNANDO A.; LINZKE, MARLEEN; KRONENBERGER, THALES; DOMLING, ALEXANDER S. S.; GROVES, MATTHEW R.; WRENGER, CARSTEN. Molecular Target Validation of Aspartate Transcarbamoylase from Plasmodium falciparum by Torin 2. ACS INFECTIOUS DISEASES, v. 6, n. 5, p. 986-999, MAY 8 2020. (17/03966-4, 15/26722-8, 13/17577-9, 14/23330-9)

DE AZEVEDO, ERIKA CHANG; NASCIMENTO, ALESSANDRO S.. Energy landscape of the domain movement in Staphylococcus aureus UDP-N-acetylglucosamine 2-epimerase. Journal of Structural Biology, v. 207, n. 2, p. 158-168, AUG 1 2019. (15/13684-0, 17/18173-0, 15/26722-8)

VASCONCELOS, STANLEY N. S.; MEISSNER, KAMILA A.; FERRAZ, WITOR R.; TROSSINI, GUSTAVO H. G.; WRENGER, CARSTEN; STEFANI, HELIO A.. Indole-3-glyoxyl tyrosine: synthesis and antimalarial activity against Plasmodium falciparum. Future Medicinal Chemistry, v. 11, n. 6, p. 525-538, MAR 2019. (13/17960-7, 12/12807-3, 15/26722-8, 12/00424-2, 17/25543-8, 16/24790-9)

BATISTA, FERNANDO A.; GYAU, BENJAMIN; VILACHA, JULIANA F.; BOSCH, SORAYA S.; LUNEV, SERGEY; WRENGER, CARSTEN; GROVES, MATTHEW R.. New directions in antimalarial target validation. EXPERT OPINION ON DRUG DISCOVERY, v. 15, n. 2, p. 189-202, FEB 1 2020. (17/03966-4, 15/26722-8, 13/17577-9)

LUNEV, SERGEY; BOSCH, SORAYA S.; BATISTA, FERNANDO A.; WANG, CHAO; LI, JINGYAO; LINZKE, MARLEEN; KRUITHOF, PAUL; CHAMOUN, GEORGE; DOMLING, ALEXANDER S. S.; WRENGER, CARSTEN; et al. Identification of a non-competitive inhibitor of Plasmodium falciparum aspartate transcarbamoylase. Biochemical and Biophysical Research Communications, v. 497, n. 3, p. 835-842, MAR 11 2018. (15/26722-8, 13/17577-9, 14/23330-9)

SUMAM DE OLIVEIRA, DAFFINY; KRONENBERGER, THALES; PALMISANO, GIUSEPPE; WRENGER, CARSTEN; DE SOUZA, EDMARCIA ELISA. Targeting SUMOylation in Plasmodium as a Potential Target for Malaria Therapy. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 11, JUN 10 2021. (18/10150-3, 20/04923-0, 19/26771-0, 20/12277-0, 17/03966-4, 18/18257-1, 18/15549-1, 15/26722-8)

DOMBROWSKI, JAMILLE GREGORIO; BARATEIRO, ANDRE; MACHADO PEIXOTO, ERIKA PAULA; CLAUDIO DA SILVA BARROS, ANDRE BOLER; DE SOUZA, RODRIGO MEDEIROS; CLARK, TAANE GREGORY; CAMPINO, SUSANA; WRENGER, CARSTEN; WUNDERLICH, GERHARD; PALMISANO, GIUSEPPE; et al. Adverse pregnancy outcomes are associated with Plasmodium vivax malaria in a prospective cohort of women from the Brazilian Amazon. PLoS Neglected Tropical Diseases, v. 15, n. 4, APR 2021. (17/03966-4, 17/03939-7, 18/18257-1, 19/12068-5, 15/26722-8, 17/24267-7, 20/06747-4, 18/20468-0, 20/04923-0, 18/15549-1, 17/05782-8)

PILLAT, MICHELI MAINARDI; KRUGER, ARNE; FERREIRA GUIMARAES, LARA MENDES; LAMEU, CLAUDIANA; DE SOUZA, EDMARCIA ELISA; WRENGER, CARSTEN; ULRICH, HENNING. Insights in Chloroquine Action: Perspectives and Implications in Malaria andCOVID-19. Cytometry Part A, v. 97, n. 9, AUG 2020. (18/07366-4, 18/08820-0, 15/26722-8, 15/19128-2)

PRATA, ISADORA OLIVEIRA; GALINDO CUBILLOS, ELIANA FERNANDA; KRUGER, ARNE; BARBOSA, DEIBS; MARTINS, JOAQUIM; SETUBAL, JOAO CARLOS; WUNDERLICH, GERHARD. Plasmodium falciparum Acetyl-CoA Synthetase Is Essential for Parasite Intraerythrocytic Development and Chromatin Modification. ACS INFECTIOUS DISEASES, v. 7, n. 12, p. 3224-3240, DEC 10 2021. (17/24267-7, 16/12659-5, 18/08820-0, 15/26722-8)

WANG, CHAO; KRUEGER, ARNE; DU, XIAOCHEN; WRENGER, CARSTEN; GROVES, MATTHEW R.. Novel Highlight in Malarial Drug Discovery: Aspartate Transcarbamoylase. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 12, p. 9-pg., 2022-03-04. (18/08820-0, 15/26722-8)

MIRANDA, RENATA RANK; FERREIRA, NATALIA NORONHA; DE SOUZA, EDMARCIA ELISA; PINCELA LINS, PAULA MARIA; BARBOZA FERREIRA, LEONARDO MIZIARA; KRUGER, ARNE; DE OLIVEIRA CARDOSO, VALERIA MARIA; DURIGON, EDISON LUIZ; WRENGER, CARSTEN; ZUCOLOTTO, VALTENCIR. Modulating Fingolimod (FTY720) Anti-SARS-CoV-2 Activity Using a PLGA-Based Drug Delivery System. ACS APPLIED BIO MATERIALS, v. N/A, p. 13-pg., 2022-06-22. (19/25645-0, 20/12277-0, 18/08820-0, 15/26722-8)

LUNEV, SERGEY; BUTZLOFF, SABINE; ROMERO, ATILIO R.; LINZKE, MARLEEN; BATISTA, FERNANDO A.; MEISSNER, KAMILA A.; MUELLER, INGRID B.; ADAWY, ALAA; WRENGER, CARSTEN; GROVES, MATTHEW R.. Oligomeric interfaces as a tool in drug discovery: Specific interference with activity of malate dehydrogenase of Plasmodium falciparum in vitro. PLoS One, v. 13, n. 4, APR 25 2018. (12/12807-3, 14/23330-9, 13/10288-1, 16/24790-9, 15/26722-8)

KRUGER, ARNE; DE JESUS SANTOS, ANA PAULA; DE SA, VANESSA; ULRICH, HENNING; WRENGER, CARSTEN. Aptamer Applications in Emerging Viral Diseases. PHARMACEUTICALS, v. 14, n. 7, JUL 2021. (18/07366-4, 15/26722-8, 17/03966-4, 15/26642-4)

REI YAN, SUN L.; WAKASUQUI, FELIPE; WRENGER, CARSTEN. Point-of-care tests for malaria: speeding up the diagnostics at the bedside and challenges in malaria cases detection. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 98, n. 3, NOV 2020. (15/26722-8, 17/26358-0)

BARRA, ANGELICA LUANA C.; ULLAH, NAJEEB; MORAO, LUANA G.; WRENGER, CARSTEN; BETZEL, CHRISTIAN; NASCIMENTO, ALESSANDRO S.. Structural Dynamics and Perspectives of Vitamin B6 Biosynthesis Enzymes in Plasmodium: Advances and Open Questions. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 11, JUL 13 2021. (18/21213-6, 19/20219-3, 20/03983-9, 15/13684-0, 19/26428-3, 15/26722-8, 17/03966-4)

FLOREZ-ALVAREZ, LIZDANY; DE SOUZA, EDMARCIA ELISA; BOTOSSO, VIVIANE FONGARO; LEAL DE OLIVEIRA, DANIELLE BRUNA; HO, PAULO LEE; TABORDA, CARLOS PELLESCHI; PALMISANO, GIUSEPPE; CAPURRO, MARGARETH LARA; REBELLO PINHO, JOAO RENATO; FERREIRA, HELENA LAGE; et al. Hemorrhagic fever viruses: Pathogenesis, therapeutics, and emerging and re-emerging potential. FRONTIERS IN MICROBIOLOGY, v. 13, p. 16-pg., 2022-10-25. (16/20045-7, 19/12303-4, 18/18257-1, 20/04923-0, 17/03966-4, 17/27131-9, 22/01812-8, 18/15549-1, 15/26722-8, 20/12277-0, 20/06409-1, 16/08730-6)

SARTORI, GERALDO RODRIGUES; NASCIMENTO, ALESSANDRO S.. Comparative Analysis of Electrostatic Models for Ligand Docking. FRONTIERS IN MOLECULAR BIOSCIENCES, v. 6, JUL 3 2019. (15/13684-0, 17/18173-0, 15/26722-8)

BATISTA, FERNANDO A.; BOSCH, SORAYA S.; BUTZLOFF, SABINE; LUNEV, SERGEY; MEISSNER, KAMILA A.; LINZKE, MARLEEN; ROMERO, ATILIO R.; WANG, CHAO; MUELLER, INGRID B.; DOMLING, ALEXANDER S. S.; et al. Oligomeric protein interference validates druggability of aspartate interconversion in Plasmodium falciparum. MICROBIOLOGYOPEN, v. 8, n. 7, JUL 2019. (17/03966-4, 15/26722-8, 16/24790-9, 14/23330-9, 13/17577-9)

LAZARI, LUCAS CARDOSO; GHILARDI, FABIO DE ROSE; ROSA-FERNANDES, LIVIA; ASSIS, DIEGO M.; NICOLAU, JOSE CARLOS; SANTIAGO, VERONICA FEIJOLI; DALCOQUIO, TALIA FALCAO; ANGELI, CLAUDIA B.; BERTOLIN, ADRIADNE JUSTI; MARINHO, CLAUDIO R. F.; et al. Prognostic accuracy of MALDI-TOF mass spectrometric analysis of plasma in COVID-19. LIFE SCIENCE ALLIANCE, v. 4, n. 8, AUG 2021. (20/04705-2, 18/15549-1, 17/03966-4, 15/26722-8, 18/20468-0, 20/04923-0, 18/18257-1)

RIBEIRO, GIOVANE DE JESUS GOMES; YAN, SUN LIU REI; PALMISANO, GIUSEPPE; WRENGER, CARSTEN. Plant Extracts as a Source of Natural Products with Potential Antimalarial Effects: An Update from 2018 to 2022. PHARMACEUTICS, v. 15, n. 6, p. 19-pg., 2023-06-01. (18/15549-1, 18/18257-1, 20/04923-0, 17/26358-0, 15/26722-8)

SILVA-SANTOS, YASMIN; PAGNI, ROBERTA LIBERATO; GAMON, THAIS HELENA MARTINS; DE AZEVEDO, MARCELA SANTIAGO PACHECO; BIELAVSKY, MONICA; DARIDO, MARIA LAURA GOUSSAIN; DE OLIVEIRA, DANIELLE BRUNA LEAL; DE SOUZA, EDMARCIA ELISA; WRENGER, CARSTEN; DURIGON, EDSON LUIZ; et al. Lisinopril increases lung ACE2 levels and SARS-CoV-2 viral load and decreases inflammation but not disease severity in experimental COVID-19. FRONTIERS IN PHARMACOLOGY, v. 15, p. 14-pg., 2024-07-12. (20/06409-1, 15/26722-8, 23/05478-8, 20/06747-4, 22/13150-0, 20/12277-0)

COUTINHO, JOAO V. P.; ROSA-FERNANDES, LIVIA; MULE, SIMON NGAO; DE OLIVEIRA, GILBERTO SANTOS; MANCHOLA, NUBIA CAROLINA; SANTIAGO, VERONICA FEIJOLI; COLLI, WALTER; WRENGER, CARSTEN; MANSO ALVES, MARIA JULIA; PALMISANO, GIUSEPPE. The thermal proteome stability profile of Trypanosoma cruzi in epimastigote and trypomastigote life stages. JOURNAL OF PROTEOMICS, v. 248, SEP 30 2021. (18/13283-4, 18/15549-1, 14/06863-3, 17/03966-4, 15/26722-8, 17/19854-0, 17/04032-5, 18/18257-1)

DE AZEVEDO, ERIKA CHANG; NASCIMENTO, ALESSANDRO S.. The beta-lactam ticarcillin is a Staphylococcus aureus UDP-N-acetylglucosamine 2-epimerase binder. Biochimie, v. 197, p. 8-pg., 2022-02-03. (17/18173-0, 15/26722-8, 15/13684-0, 20/03983-9)

DE SOUZA, JOAO VICTOR; NOGUEIRA, VICTOR H. R.; NASCIMENTO, ALESSANDRO S.. Ligand binding free energy evaluation by Monte Carlo Recursion. COMPUTATIONAL BIOLOGY AND CHEMISTRY, v. 103, p. 12-pg., 2023-02-20. (17/18173-0, 20/03983-9, 14/06565-2, 10/15376-8, 14/01751-2, 15/01709-9, 15/26722-8)

TAVARES, MAURICIO T.; KRUEGER, ARNE; YAN, SUN L. REI; WAITMAN, KAROLINE B.; GOMES, VINICIUS M.; DE OLIVEIRA, DAFFINY SUMAM; PAZ, FRANCIARLI; HILSCHER, SEBASTIAN; SCHUTKOWSKI, MIKE; SIPPL, WOLFGANG; et al. 1,3-Diphenylureido hydroxamate as a promising scaffold for generation of potent antimalarial histone deacetylase inhibitors. SCIENTIFIC REPORTS, v. 13, n. 1, p. 20-pg., 2023-11-30. (18/08820-0, 18/10150-3, 20/04923-0, 21/00124-8, 18/18257-1, 21/08260-8, 15/26722-8, 18/15549-1, 22/12468-6, 17/26358-0, 21/11606-3, 17/03966-4, 22/07275-4)

REINKE, PATRICK Y. A.; DE SOUZA, EDMARCIA ELISA; GUENTHER, SEBASTIAN; FALKE, SVEN; LIESKE, JULIA; EWERT, WIEBKE; LOBODA, JURE; HERRMANN, ALEXANDER; MASHHOUR, AIDA RAHMANI; KARNICAR, KATARINA; et al. Calpeptin is a potent cathepsin inhibitor and drug candidate for SARS-CoV-2 infections. COMMUNICATIONS BIOLOGY, v. 6, n. 1, p. 13-pg., 2023-10-18. (21/02736-0, 20/09149-0, 15/26722-8, 20/07251-2, 22/01812-8, 19/00899-0, 20/12277-0)

LINZKE, MARLEEN; YAN, SUN LIU REI; TARNOK, ATTILA; ULRICH, HENNING; GROVES, MATTHEW R.; WRENGER, CARSTEN. Live and Let Dye: Visualizing the Cellular Compartments of the Malaria Parasite Plasmodium falciparum. Cytometry Part A, v. 97, n. 7, p. 12-pg., 2019-11-18. (17/03966-4, 12/50880-4, 14/23330-9, 12/50393-6, 17/26358-0, 15/26722-8)

WANG, MENGYING; BARRA, ANGELICA LUANA C.; BROGNARO, HEVILA; BETZEL, CHRISTIAN. Exploring Nucleation Pathways in Distinct Physicochemical Environments Unveiling Novel Options to Modulate and Optimize Protein Crystallization. CRYSTALS, v. 12, n. 3, p. 22-pg., 2022-03-01. (18/21213-6, 15/26722-8, 19/26428-3)

LINZKE, MARLEEN; YAN, SUN LIU REI; TARNOK, ATTILA; ULRICH, HENNING; GROVES, MATTHEW R.; WRENGER, CARSTEN. Live and Let Dye: Visualizing the Cellular Compartments of the Malaria Parasite Plasmodium falciparum. Cytometry Part A, v. 97, n. 7, SI, NOV 2019. (12/50880-4, 17/03966-4, 15/26722-8, 14/23330-9, 17/26358-0, 12/50393-6)

BORGES-PEREIRA, LUCAS; MEISSNER, KAMILA ANNA; WRENGER, CARSTEN; GARCIA, CELIA R. S.. Plasmodium falciparum GFP-E-NTPDase expression at the intraerythrocytic stages and its inhibition blocks the development of the human malaria parasite. PURINERGIC SIGNALLING, v. 13, n. 3, p. 267-277, SEP 2017. (12/12807-3, 10/51593-3, 15/26722-8, 13/10288-1, 11/51295-5)

SOUZA JUNIOR, DOUGLAS RICARDO; MARTINS SILVA, AMANDA RIBEIRO; ROSA-FERNANDES, LIVIA; REIS, LORENNA ROCHA; ALEXANDRIA, GABRIELLY; BHOSALE, SANTOSH D.; GHILARDI, FABIO DE ROSE; DALCOQUIO, TALIA FALCAO; BERTOLIN, ADRIADNE JUSTI; NICOLAU, JOSE CARLOS; et al. HDL proteome remodeling associates with COVID-19 severity. JOURNAL OF CLINICAL LIPIDOLOGY, v. 15, n. 6, p. 796-804, NOV-DEC 2021. (15/26722-8, 16/00696-3, 20/04923-0, 19/25702-4, 17/07725-1, 18/15549-1, 18/18257-1, 13/07937-8)

MORAO, LUANA G.; MANZINE, LIVIA R.; CLEMENTINO, LIVIA OLIVEIRA D.; WRENGER, CARSTEN; NASCIMENTO, ALESSANDRO S.. A scalable screening of E. coli strains for recombinant protein expression. PLoS One, v. 17, n. 7, p. 10-pg., 2022-07-25. (17/24901-8, 17/03966-4, 15/26722-8, 19/20219-3, 20/03983-9)

MINARI, KARINE; DE AZEVEDO, ERIKA CHANG; RODRIGUES KIRALY, VANESSA THOMAZ; HELENO BATISTA, FERNANDA APARECIDA; DE MORAES, FABIO ROGERIO; DE MELO, FERNANDO ALVES; NASCIMENTO, ALESSANDRO SILVA; GAVA, LISANDRA MARQUES; INACIO RAMOS, CARLOS HENRIQUE; BORGES, JULIO CESAR. Thermodynamic analysis of interactions of the Hsp90 with adenosine nucleotides: A comparative perspective. International Journal of Biological Macromolecules, v. 130, p. 125-138, JUN 1 2019. (15/26722-8, 13/25646-0, 17/18173-0, 11/23110-0, 14/07206-6, 12/50161-8, 09/53989-4, 17/07335-9)

KRUGER, ARNE; ZIMBRES, FLAVIA M.; KRONENBERGER, THALES; WRENGER, CARSTEN. Molecular Modeling Applied to Nucleic Acid-Based Molecule Development. BIOMOLECULES, v. 8, n. 3, SEP 2018. (17/03966-4, 18/08820-0, 15/26722-8)

MEISSNER, KAMILA ANNA; KRONENBERGER, THALES; MALTAROLLO, VINICIUS GONCALVES; GOULART TROSSINI, GUSTAVO HENRIQUE; WRENGER, CARSTEN. Targeting the Plasmodium falciparum plasmepsin V by ligand-based virtual screening. CHEMICAL BIOLOGY & DRUG DESIGN, v. 93, n. 3, p. 300-312, MAR 2019. (14/03644-9, 16/24790-9, 17/03966-4, 15/26722-8)

SCARPELLI, PEDRO H.; TESSARIN-ALMEIDA, GIULLIANA; VICOSO, KENIA LOPES; LIMA, WANIA REZENDE; BORGES-PEREIRA, LUCAS; MEISSNER, KAMILA ANNA; WRENGER, CARSTEN; RAFAELLO, ANNA; RIZZUTO, ROSARIO; POZZAN, TULLIO; et al. Melatonin activates FIS1, DYN1, and DYN2 Plasmodium falciparum related-genes for mitochondria fission: Mitoemerald-GFP as a tool to visualize mitochondria structure. Journal of Pineal Research, v. 66, n. 2, MAR 2019. (12/12807-3, 11/51295-5, 14/14347-5, 15/26722-8)

GAO, KAI; WANG, WENJIA; KRONENBERGER, THALES; WRENGER, CARSTEN; GROVES, MATTHEW R.. The Crystal Structure of the Plasmodium falciparum PdxK Provides an Experimental Model for Pro-Drug Activation. CRYSTALS, v. 9, n. 10, OCT 2019. (17/03966-4, 14/03644-9, 15/26722-8)

DE PAULA, KARINA; SANTOS, JADEMILSON C.; MAFUD, ANA CAROLINA; NASCIMENTO, ALESSANDRO S.. Tetrazoles as PPAR gamma ligands: A structural and computational investigation. JOURNAL OF MOLECULAR GRAPHICS & MODELLING, v. 106, JUL 2021. (10/15376-8, 20/03983-9, 14/06565-2, 15/26722-8)

BOTOSSO, VIVIANE FONGARO; DURIGON, EDISON LUIZ; DE SOUZA, EDMARCIA ELISA. Emerging human viruses with pandemic potential: Diagnostics, pathogenesis, and therapeutics. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 13, p. 2-pg., 2023-03-23. (20/12277-0, 21/11946-9, 15/26722-8)

GOMES, LAURA CORDEIRO; SANSON, MARINA CORDEIRO GOMES; BRAININ, PHILIP; DE MELO, MARIA DA CONCEICAO VIEIRA; DE SOUZA, RODRIGO MEDEIROS; MAZARO, JANAINA; LIMA, KARINE OLIVEIRA; RESENDE, JUNIA SILVA; VIEIRA, ISABELLE VICTORIA MARTINS; MESQUITA, EDINILSON DA SILVA; et al. Levels of hepatitis B antibody titers are affected by age and doses gap time in children from a high endemic area of the western Amazon. PLoS One, v. 16, n. 7, 2021. (18/20468-0, 18/18257-1, 18/15549-1, 15/26722-8, 14/06863-3)

LAZARI, LUCAS C.; ZERBINATI, RODRIGO M.; ROSA-FERNANDES, LIVIA; SANTIAGO, VERONICA FEIJOLI; ROSA, KLAISE F.; ANGELI, CLAUDIA B.; SCHWAB, GABRIELA; PALMIERI, MICHELLE; SARMENTO, DMITY J. S.; MARINHO, CLAUDIO R. F.; et al. ALDI-TOF mass spectrometry of saliva samples as a prognostic tool for COVID-1. OURNAL OF ORAL MICROBIOLOG, v. 14, n. 1, DEC 31 2022. (18/15549-1, 20/06409-1, 20/04923-0, 18/18257-1, 15/26722-8, 17/03966-4, 21/07490-0, 18/20468-0)

BARRA, ANGELICA LUANA C.; DANTAS, LIVIA DE OLIVEIRA C.; MORAO, LUANA GALVAO; GUTIERREZ, RAISSA F.; POLIKARPOV, IGOR; WRENGER, CARSTEN; NASCIMENTO, ALESSANDRO S.. Essential Metabolic Routes as a Way to ESKAPE From Antibiotic Resistance. FRONTIERS IN PUBLIC HEALTH, v. 8, FEB 28 2020. (15/13684-0, 17/18173-0, 15/26722-8)

MACEDO-DA-SILVA, JANAINA; ROSA-FERNANDES, LIVIA; GOMES, VINICIUS DE MORAIS; SANTIAGO, VERONICA FEIJOLI; SANTOS, DEIVID MARTINS; MOLNAR, CATARINA MARIA STANISCHESK; BARBOZA, BRUNO RAFAEL; DE SOUZA, EDMARCIA ELISA; MARQUES, RODOLFO FERREIRA; BOSCARDIN, SILVIA BEATRIZ; et al. Protein Arginylation Is Regulated during SARS-CoV-2 Infection. Viruses-Basel, v. 15, n. 2, p. 25-pg., 2023-02-01. (18/15549-1, 18/18257-1, 20/06409-1, 22/09915-0, 20/02988-7, 21/00140-3, 20/12277-0, 21/14179-9, 15/26722-8)

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