Sequencing of the Genome, transcriptoma and phenotypic analysis of Campylobacter coli strains isolated from different sources in Brazil

• Grant number: 16/24716-3
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2017
• End date: August 31, 2019
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Juliana Pfrimer Falcão
• Grantee: Juliana Pfrimer Falcão
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Campylobacteriosis is a zoonosis of worldwide distribution with significant repercussions on the level of public health and with a high socioeconomic impact. C. coli and C. jejuni species are common causes of gastroenteritis in humans affecting annually approximately 2.4 million people in the United States and an estimated of 9 million people in Europe. However, the pathogenesis process of this bacterial genus is not fully known and among the few studies performed with this purpose, the majority has been performed for C. jejuni. Specifically in Brazil, the study and isolation of C. coli are not frequent, making it difficult to evaluate the importance of this pathogen in this country. The aims of this project are to comparatively analyze, by phenotypic tests, sequencing of the genome and transcriptome, 50 C. coli strains isolated from humans, animals, the environment and food during 16 years in Brazil. It will be performed for all strains phenotypic tests of temperature fluctuation, resistance to NaCl, acidic and oxidative stress, invasion to epithelial cells, and proliferation and survival in human (U937) and chickens (HD11) macrophages and analysis of the expression of inflammatory cytokine transcripts in CACO-2 cells by RT-qPCR for strains that present significant results in the cited above tests. It will be done the whole genome sequencing (WGS) of 50 C. coli strains of and the transcriptome sequencing (RNA seq) of two strains of C. coli. For the RNA seq assay two strains of clinical and non-clinical origin will be chosen based on the significant differences in the phenotypic tests cited above. The RNAs of these strains will be extracted after tolerance to acid stress and invasion to Caco-2 cells assays. Moreover, the median lethal dose (LD50) of two strains of C. coli will be performed. Due to the paucity of studies involving the C. coli species, studies that aim to elucidate the mechanisms of pathogenicity and virulence are unpublished and of great importance. The results to be obtained should contribute for the better characterization of C. coli isolated from diverse sources during 16 years in Brazil, contributing for a better understanding of the genotypic and phenotypic diversity, pathogenicity and virulence of this pathogen considered of great importance worldwide. (AU)

Scientific publications

(27)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARTINS IMORI, PRISCILLA FERNANDA; CAMPIONI, FABIO; CAO, GUOJIE; KASTANIS, GEORGE; LEON, MARIA SANCHEZ; ALLARD, MARC WILLIAM; FALCAO, JULIANA PFRIMER. Draft Genome Sequences of Yersinia frederiksenii, Yersinia intermedia, and Yersinia kristensenii Strains from Brazil. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 5, n. 32, AUG 2017. (16/05817-3, 16/24716-3, 13/25191-3)

CAMPIONI, FABIO; GOMES, CAROLINA NOGUEIRA; RODRIGUES, DALIA DOS PRAZERES; BERGAMINI, ALZIRA MARIA MORATO; FALCAO, JULIANA PFRIMER. henotypic analyses of Salmonella enterica serovar Enteritidis strains isolated in the pre- and post-epidemic period in Brazi. Brazilian Journal of Microbiology, v. 52, n. 1, SI, OCT 2020. (16/24716-3, 13/25191-3)

SERIBELLI, AMANDA AP.; DA SILVA, PATRICK; DA CRUZ, MARCELO FERREIRA; DE ALMEIDA, FERNANDA; FRAZAO, MILIANE R.; MEDEIROS, MARTA I. C.; RODRIGUES, DALIA DOS P.; KICH, JALUSA D.; DE JESUS BENEVIDES, LEANDRO; SOARES, SIOMAR DE C.; et al. nsights about the epidemiology of Salmonella Typhimurium isolates from different sources in Brazil using comparative genomic. GUT PATHOGENS, v. 13, n. 1, APR 28 2021. (16/24716-3, 19/19338-8, 17/06633-6)

SERIBELLI, AMANDA APARECIDA; CRUZ, MARCELO FERREIRA; VILELA, FELIPE PINHEIRO; FRAZAO, MILIANE RODRIGUES; PAZIANI, MARIO H.; ALMEIDA, FERNANDA; CAZENTINI MEDEIROS, MARTA INES; RODRIGUES, DALIA DOS PRAZERES; VON ZESKA KRESS, MARCIA R.; ALLARD, MARC W.; et al. Phenotypic and genotypic characterization of Salmonella Typhimurium isolates from humans and foods in Brazil. PLoS One, v. 15, n. 8, AUG 18 2020. (16/24716-3, 17/06633-6)

CAMPIONI, FABIO; VILELA, FELIPE PINHEIRO; CAO, GUOJIE; KASTANIS, GEORGE; RODRIGUES, DALIA DOS PRAZERES; COSTA, RENATA GARCIA; TIBA-CASAS, MONIQUE RIBEIRO; YIN, LANLAN; ALLARD, MARC; FALCAO, JULIANA PFRIMER. Whole genome sequencing analyses revealed that Salmonella enterica serovar Dublin strains from Brazil belonged to two predominant clades. SCIENTIFIC REPORTS, v. 12, n. 1, p. 6-pg., 2022-06-22. (16/05817-3, 16/24716-3, 13/25191-3, 19/19338-8)

GOMES, CAROLINA N.; CAMPIONI, FABIO; VILELA, FELIPE P.; DUQUE, SHEILA S.; FALCAO, JULIANA P.. Campylobacter coli strains from Brazil can invade phagocytic and epithelial cells and induce IL-8 secretion. Brazilian Journal of Microbiology, v. 52, n. 2, p. 859-867, JUN 2021. (15/23408-0, 16/24716-3)

GOMES, CAROLINA N.; FRAZAO, MILIANE R.; PASSAGLIA, JAQUELINE; DUQUE, SHEILA S.; CAZENTINI MEDEIROS, I, MARTA; FALCAO, JULIANA P.. Molecular Epidemiology and Resistance Profile of Campylobacter jejuni and Campylobacter coli Strains Isolated from Different Sources in Brazil. MICROBIAL DRUG RESISTANCE, v. 26, n. 12, DEC 2019. (14/13029-0, 16/24716-3)

VILELA, FELIPE PINHEIRO; RODRIGUES, DALIA DOS PRAZERES; COSTA, RENATA GARCIA; TIBA CASAS, MONIQUE RIBEIRO; FALCAO, JULIANA PFRIMER; CAMPIONI, FABIO. High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33-year period in Brazil. Brazilian Journal of Microbiology, v. 51, n. 2, NOV 2019. (17/05756-7, 19/06947-6, 13/25191-3, 16/24716-3)

CAMPIONI, FABIO; GOMES, CAROLINA N.; BERGAMINI, ALZIRA M. M.; RODRIGUES, DALIA P.; FALCAO, JULIANA P.. Partial Correlation Between Phenotypic and Genotypic Antimicrobial Resistance of Salmonella enterica Serovar Enteritidis Strains from Brazil. MICROBIAL DRUG RESISTANCE, v. 26, n. 12, APR 2020. (13/25191-3, 16/24716-3)

SERIBELLI, AMANDA APARECIDA; FRAZAO, MILIANE RODRIGUES; GONZALES, JOLIA CUNHA; CAO, GUOJIE; LEON, MARIA SANCHEZ; KICH, JALUSA DEON; ALLARD, MARC WILLIAM; FALCAO, JULIANA PFRIMER. Draft Genome Sequences of 20 Salmonella enterica subsp. enterica Serovar Typhimurium Strains Isolated from Swine in Santa Catarina, Brazil. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 6, n. 16, APR 2018. (16/24716-3)

CAMPIONI, FABIO; VILELA, FELIPE PINHEIRO; CAO, GUOJIE; KASTANIS, GEORGE; MILLER, DANIELA; LEON, MARIA SANCHEZ; TIBA-CASAS, MONIQUE RIBEIRO; FERNANDES, SUELI APARECIDA; RODRIGUES, DANA DOS PRAZERES; COSTA, RENATA GARCIA; et al. Draft Genome Sequences of 112 Salmonella enterica Serovar Dublin Strains Isolated from Humans and Animals in Brazil. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 6, n. 24, JUN 2018. (17/05756-7, 16/05817-3, 15/10818-6, 16/24716-3, 13/25191-3)

SERIBELLI, AMANDA APARECIDA; MACHADO RIBEIRO, TAMARA R.; DA SILVA, PATRICK; MARTINS, ISABELA MANCINI; VILELA, FELIPE PINHEIRO; CAZENTINI MEDEIROS, I, MARTA; PERONNI, KAMILA CHAGAS; DA SILVA JUNIOR, WILSON ARAUJO; MOREIRA, CRISTIANO GALLINA; FALCAO, JULIANA PFRIMER. Salmonella Typhimurium ST313 isolated in Brazil revealed to be more invasive and inflammatory in murine colon compared to ST19 strains. JOURNAL OF MICROBIOLOGY, v. 59, n. 9, AUG 2021. (17/06633-6, 16/12744-2, 19/19338-8, 19/03049-7, 16/24716-3)

VILELA, FELIPE PINHEIRO; GOMES, CAROLINA NOGUEIRA; PAZIANI, MARIO HENRIQUE; BRAZ, VANIA SANTOS; RODRIGUES, DALIA DOS PRAZERES; COSTA, RENATA GARCIA; TIBA-CASAS, MONIQUE RIBEIRO; VON ZESKA KRESS, MARCIA REGINA; FALCAO, JULIANA PFRIMER; CAMPIONI, FABIO. Virulence traits and expression of bstA, fliC and sopE2 in Salmonella Dublin strains isolated from humans and animals in Brazil. INFECTION GENETICS AND EVOLUTION, v. 80, JUN 2020. (13/25191-3, 17/05756-7, 19/06947-6, 16/24716-3)

SERIBELLI, AMANDA AP.; DA SILVA, PATRICK; DA CRUZ, MARCELO FERREIRA; DE ALMEIDA, FERNANDA; FRAZAO, MILIANE R.; MEDEIROS, MARTA I. C.; RODRIGUES, DALIA DOS P.; KICH, JALUSA D.; DE JESUS BENEVIDES, LEANDRO; SOARES, SIOMAR DE C.; et al. Insights about the epidemiology of Salmonella Typhimurium isolates from different sources in Brazil using comparative genomics. GUT PATHOGENS, v. 13, n. 1, p. 15-pg., 2021-04-28. (17/06633-6, 16/24716-3, 19/19338-8)

VILELA, FELIPE PINHEIRO; FALCAO, JULIANA PFRIMER; CAMPIONI, FABIO. Analysis of Resistance Gene Prevalence in Whole-Genome Sequenced Enterobacteriales from Brazil. MICROBIAL DRUG RESISTANCE, v. 26, n. 6, NOV 2019. (19/06947-6, 16/24716-3)

CAMPIONI, F.; GOMES, C. N.; BERGAMINI, A. M. M.; RODRIGUES, D. P.; TIBA-CASAS, M. R.; FALCAO, J. P.. Comparison of cell invasion, macrophage survival and inflammatory cytokines profiles between Salmonella enterica serovars Enteritidis and Dublin from Brazil. Journal of Applied Microbiology, v. 130, n. 6, NOV 2020. (16/24716-3)

GOMES, CAROLINA N.; PASSAGLIA, JAQUELINE; VILELA, FELIPE P.; PEREIRA DA SILVA, FATIMA M. H. S.; DUQUE, SHEILA S.; FALCAO, JULIANA P.. High survival rates of Campylobacter coli under different stress conditions suggest that more rigorous food control measures might be needed in Brazil. FOOD MICROBIOLOGY, v. 73, p. 327-333, AUG 2018. (15/23408-0, 16/24716-3)

AP SERIBELLI, AMANDA; GONZALES, JULIA C.; DE ALMEIDA, FERNANDA; BENEVIDES, LEANDRO; CAZENTINI MEDEIROS, I, MARTA; RODRIGUES, DALIA DOS PRAZERES; SOARES, SIOMAR DE C.; ALLARD, MARC W.; FALCAO, JULIANA P.. Phylogenetic analysis revealed that Salmonella Typhimurium ST313 isolated from humans and food in Brazil presented a high genomic similarity. Brazilian Journal of Microbiology, v. 51, n. 1, p. 53-64, MAR 2020. (16/24716-3, 17/06633-6)

GOMES, CAROLINA NOGUEIRA; BARKER, DILLON OLIVER REESE; DUQUE, SHEILA DA SILVA; CHE, EMILY VICTORIA; JAYAMANNA, VASENA; TABOADA, EDUARDO NAPOLEON; FALCAO, JULIANA PFRIMER. Campylobacter coli isolated in Brazil typed by core genome Multilocus Sequence Typing shows high genomic diversity in a global context. INFECTION GENETICS AND EVOLUTION, v. 95, NOV 2021. (18/26043-1, 16/24716-3, 15/23408-0, 19/19338-8)

SERIBELLI, AMANDA APARECIDA; DA SILVA, PATRICK; FRAZAO, MILIANE RODRIGUES; KICH, JALUSA DEON; ALLARD, MARC W.; FALCAO, JULIANA PFRIMER. Phylogenetic relationship and genomic characterization of Salmonella Typhimurium strains isolated from swine in Brazil. INFECTION GENETICS AND EVOLUTION, v. 93, SEP 2021. (16/24716-3, 17/06633-6, 19/19338-8)

BRAZ, VANIA SANTOS; RUEDA FURLAN, JOAO PEDRO; PASSAGLIA, JAQUELINE; FALCAO, JULIANA PFRIMER; STEHLING, ELIANA GUEDES. Genotypic diversity and presence of beta-lactamase encoding genes in Pseudomonas aeruginosa isolated from Brazilian soils. APPLIED SOIL ECOLOGY, v. 129, p. 94-97, AUG 2018. (15/18990-2, 16/24716-3)

FRAZAO, MILIANE RODRIGUES; CAO, GUOJIE; MEDEIROS, MARTA INES CAZENTINI; DUQUE, SHEILA DA SILVA; ALLARD, MARC WILLIAM; FALCAO, JULIANA PFRIMER. Antimicrobial Resistance Profiles and Phylogenetic Analysis ofCampylobacter jejuniStrains Isolated in Brazil by Whole Genome Sequencing. MICROBIAL DRUG RESISTANCE, v. 27, n. 5, p. 660-669, MAY 1 2021. (14/13029-0, 16/24716-3, 18/06904-2, 19/19338-8)

SERIBELLI, AMANDA APARECIDA; FRAZAO, MILIANE RODRIGUES; GONZALES, JOLIA CUNHA; CAO, GUOJIE; LEON, MARIA SANCHEZ; KICH, JALUSA DEON; ALLARD, MARC WILLIAM; FALCAO, JULIANA PFRIMER. Draft Genome Sequences of 20 Salmonella enterica subsp. enterica Serovar Typhimurium Strains Isolated from Swine in Santa Catarina, Brazil. GENOME ANNOUNCEMENTS, v. 6, n. 16, p. 2-pg., 2018-04-01. (16/24716-3)

CAMPIONI, FABIO; VILELA, FELIPE PINHEIRO; CAO, GUOJIE; KASTANIS, GEORGE; MILLER, DANIELA; LEON, MARIA SANCHEZ; TIBA-CASAS, MONIQUE RIBEIRO; FERNANDES, SUELI APARECIDA; RODRIGUES, DANA DOS PRAZERES; COSTA, RENATA GARCIA; et al. Draft Genome Sequences of 112 Salmonella enterica Serovar Dublin Strains Isolated from Humans and Animals in Brazil. GENOME ANNOUNCEMENTS, v. 6, n. 24, p. 4-pg., 2018-06-01. (17/05756-7, 15/10818-6, 16/24716-3, 16/05817-3, 13/25191-3)

MARTINS IMORI, PRISCILLA FERNANDA; CAMPIONI, FABIO; CAO, GUOJIE; KASTANIS, GEORGE; LEON, MARIA SANCHEZ; ALLARD, MARC WILLIAM; FALCAO, JULIANA PFRIMER. Draft Genome Sequences of Yersinia frederiksenii, Yersinia intermedia, and Yersinia kristensenii Strains from Brazil. GENOME ANNOUNCEMENTS, v. 5, n. 32, p. 2-pg., 2017-08-01. (13/25191-3, 16/24716-3, 16/05817-3)

CAMPIONI, FABIO; GOMES, CAROLINA NOGUEIRA; RODRIGUES, DALIA DOS PRAZERES; BERGAMINI, ALZIRA MARIA MORATO; FALCAO, JULIANA PFRIMER. Phenotypic analyses of Salmonella enterica serovar Enteritidis strains isolated in the pre- and post-epidemic period in Brazil. Brazilian Journal of Microbiology, v. 52, n. 1, p. 11-pg., 2020-10-26. (13/25191-3, 16/24716-3)

GOMES, CAROLINA NOGUEIRA; CAMPIONI, FABIO; BARKER, DILLON OLIVER REESE; CHE, EMILY VICTORIA; DUQUE, SHEILA DA SILVA; TABOADA, EDUARDO NAPOLEON; FALCAO, JULIANA PFRIMER. Antimicrobial resistance genotypes and phenotypes of Campylobacter coli isolated from different sources over a 16-year period in Brazil. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 33, p. 5-pg., 2023-06-01. (22/07013-0, 18/26043-1, 16/24716-3, 19/19338-8, 15/23408-0)