Effects of physical exercise on inflammatory response of patients with myocardial infarction

• Grant number: 16/22073-8
• Support Opportunities: Regular Research Grants
• Duration: April 01, 2017 - March 31, 2020
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Tania Cristina Pithon Curi
• Grantee: Tania Cristina Pithon Curi
• Host Institution: Pró-Reitoria de Pós-Graduação e Pesquisa. Universidade Cruzeiro do Sul (UNICSUL). São Paulo , SP, Brazil
• Associated researchers: Adriana Cristina Levada-Pires ; André Luis Lacerda Bachi ; Mario Hiroyuki Hirata ; Renata Gorjao ; Rui Curi ; Sonia de Quateli Doi

Abstract

In recent decades, the beneficial effects of exercise have been shown as a factor that improves the quality of life of its practitioners. The American College of Sports Medicine and the American Heart Association recommend the practice of aerobic training for patients with myocardial infarction. It is known that the intensity, duration and frequency of exercise play an important role on the modulation of the immune response. Our group was the first to show that moderate exercise has anti-inflammatory effect in rats and that intense physical exercise reduces this effect by inducing apoptosis in rat and humans neutrophils. However, it was still not observed if there is modulation of the inflammatory response and cell death of neutrophils induced by exercise training in patients with myocardial infarction with or without diabetes. In this study we will investigate the effects of chronic exercise on inflammatory response in diabetic infarcted patients aged 50-70 years. The plasma concentration of pro and anti-inflammatory cytokines and C-reactive protein will be evaluated. In addition, the following parameters will be determined in neutrophils: 1) viability, 2) cytoplasmic membrane phosphatidylserine externalization, 3) DNA fragmentation, 4) potential of mitochondrial membrane 5) expression of TNF-1 receptor (TNF-R1 / CD120a), Fas (CD95 / Apo-1), TRAIL (TNF-Related Apoptosis-Inducing Ligand) 1 (TRAIL-R1 / Apo-2 / DR4) and 2 (TRAIL-R2 / DR5 / KILLER / TRICK2) on the surface of neutrophils by flow cytometry; 6) expression of miRNAs involved in pathways of cytokines and receptors that showed alterations by quantitative real-time PCR (qPCR); 7) Activity of caspases 3, 7 and 9, using the technique of Western blotting; 8) production of reactive oxygen species and 9) phagocytic capacity. (AU)