Vertical infection by the virus Zika and its impact on the mother-child area

• Grant number: 16/08578-0
• Support Opportunities: Research Projects - Thematic Grants
• Start date: April 01, 2017
• End date: April 30, 2023
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Saulo Duarte Passos
• Grantee: Saulo Duarte Passos
• Host Institution: Faculdade de Medicina de Jundiaí (FMJ). Prefeitura Municipal de Jundiaí. Jundiaí , SP, Brazil
• Pesquisadores principais: Antonio Fernandes Moron
• Associated researchers: Adriana Cruanes Mingotti ; Alexandra Siqueira Mello ; Ana Paula Antunes Pascalicchio Bertozzi ; Andre Prado Grion ; Andréa Cristina Botelho Silva ; Annemeri Livinalli ; Antonio Carlos de Quadros Junior ; Clóvis Antonio Lopes Pinto ; Danielle Bruna Leal de Oliveira ; Danila Soares Tambalo ; Dora Selma Fix Ventura ; Edison Luiz Durigon ; Eduardo José Caldeira ; Eduardo Massad ; Emmanuel Machado Oliveira ; Fábio Valente Rizzo ; Felipe Favorette Campanharo ; Fernanda Alves Cangerana Pereira ; Fernanda Guerra Velasco ; Francisco del Moral Hernandez ; Geovane Ribeiro Santos ; Gilberto Lazaroni Theodoro da Cunha ; Hérbene José Figuinha Milani ; Hyun Mo Yang ; Karen Richter Comandulli ; Laura Cunha Rodrigues ; Leslie Domenici Kulikowski ; Lourival Benedito de Oliveira ; Luiz Dias da Silva ; Magda Maria Sales Carneiro-Sampaio ; Marcia Borges Machado ; Márcia Elisabeth Rodrigues ; Margareth Martha Arilha Silva ; Maria Emília De Domenico Garcia ; Maria Manoela Duarte Rodrigues ; Marielze Pinto da Paixão ; Mauricio Mendes Barbosa ; Mayana Zatz ; Milene Tavares Batista ; Mirella Nayane Barbosa Leite ; Nemésio Florence Vieira Filho ; Paolo Marinho de Andrade Zanotto ; Patricia Carvalho Loiola ; Patricia Cristina Baleeiro Beltrão Braga ; Patricia Palmeira Daenekas Jorge ; Rafael Izbicki ; Raquel Prestes ; Renata Maria Mizani ; Rita de Cássia de Aguirre Bernardes Dezena ; Ronei Luciano Mamoni ; Rosa Estela Gazeta ; Sandra Helena Alves Bonon ; Stéphanno Gomes Pereira Sarmento ; Thamirys Cosmo Grillo Fajardo ; Viviane Cristina Martori Pandini
• Associated research grant(s): 20/05298-1 - Multi-User Equipment approved in the process 2018/08578-0: brainstem evoked response audiometer, AP.EMU; 18/15031-2 - Immune and inflammatory repercussions of congenital infections in the perinatal period, AV.EXT
• Associated scholarship(s): 20/04342-7 - Seroepidemiology of Mayaro Virus in high-risk pregnant women from the Zika Jundiaí Cohort seropositive for Chikungunya Virus, BP.IC; 20/08613-5 - The role of nursing in improving the quality of prenatal care, delivery and puerperium in pregnant women with Zika virus infection, BP.TT; 19/21144-7 - Vertical infection by the Virus Zika and its impact on the mother-child area, BP.PD; + associated scholarships 19/08399-6 - Prevalence of congenital anomalies in children born at the Hospital Universitário de Jundiaí, from 2006 to 2016, BP.IC; 19/04883-0 - The role of nursing in improving the quality of prenatal care, delivery and puerperium in pregnant women with Zika virus infection., BP.TT; 18/20917-0 - Vertical infection by the Zika virus and its repercussions in the maternal-infant area, BP.TT; 18/20918-6 - Vertical infection by the Zika virus and its repercussions in the maternal-infant area, BP.TT; 18/06131-3 - Prevention, knowledge and practice about Zika virus among pregnant women and new mothers - Jundiaí, BP.IC; 17/22007-8 - Vertical infection by the virus Zika and its impact on the mother-child area, BP.PD - associated scholarships

Abstract

The maternal-fetal transmission of viruses is well known to cause serious consequences to the fetus during pregnancy. Although there is a link between the Zika Virus (ZIKV) and microcephaly, the specter of other associations of pregnancy and birth defects need to be clarified during the first few months or years of life of children. Global alerts have been issued in this regard. Our objectives determine the incidence of infection caused by ZIKV in pregnant women and neonates born in the cohort, as well as analyze, describe the spectrum of clinical presentation of pregnant women ZIKV (+) and identify, describe and quantify the spectrum of anomalies and/or results, including microcephaly, in fetuses and newborn babies of pregnant women participating in the cohort. To develop these project, a prospective cohort study nested case-control as well as qualitative study of maternal mental health will be conducted at the University Hospital of the Faculty of Medicine of Jundiaí from 2016-2021. The sampling stipulated of the 500 pregnant women will be divided into three groups: Group I (high-risk pregnant women without symptoms); Group II (low-risk pregnant women with symptoms); and Group III (abortions); and their newborns (RN) divided into those with or without microcephaly. The RN will be divided by maternal gestational age and will be used logistic regression controlled for possible confounders. Following these, we will assess the psychomotor development and the occurrence of hearing and visual losses, and neurological involvement. Active monitoring of cases will be done weekly by telephone of pregnant women. The biological samples (blood, urine, saliva and cerebrospinal fluid) will be collected and examined by serological tests: ELISA Microarray, RT-PCR and RNA-Sequencing. The human tissues will be analyzed by pathology. Predictors will aim to calculate the risk relative, the risk univariate and multiple logistic models, when appropriate. Altogether, data will allow correlating the incidence of Zika infection and its potential as a causative agent of newborn and infant mental/physical health disorders. The project is innovative and will help researchers to understand the impact of this emergent disease on the population, especially in children. (AU)

Scientific publications

(12)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PIRES, LUCAS C.; DANTAS, LUIZA R.; WITKIN, STEVEN S.; BERTOZZI, ANA PAULA A. P.; DEZENA, RITA DE CASSIA A. B.; RODRIGUES, MARIA M. D.; GAZETA, ROSA ESTELA; PASSOS, SAULO D.. Knowledge of Zika Virus Transmission and Its Prevention among High-Risk Pregnant Women in Brazil. Viruses-Basel, v. 13, n. 2, FEB 2021. (16/08578-0)

GRILLO FAJARDO, THAMIRYS COSMO; GAZETA, ROSA ESTELA; CATALAN, DANIEL THOME; MELLO, ALEXANDRA SIQUEIRA; BOTELHO DA SILVA, ANDREA CRISTINA; PASCALICCHIO BERTOZZI, ANA PAULA ANTUNES; DOS SANTOS, GEOVANE RIBEIRO; LOPES PINTO, CLOVIS ANTONIO; MONTEIRO, CAIRO OLIVEIRA; GUARAGNA MACHADO, RAFAEL RAHAL; et al. Neonatal consequences of maternal exposure to the chikungunya virus Case reports. MEDICINE, v. 100, n. 17, APR 30 2021. (16/08578-0)

CLEMENTE, NURIA SANCHEZ; BRICKLEY, ELIZABETH B.; PAIXAO, ENNY S.; DE ALMEIDA, MARCIA F.; GAZETA, ROSA E.; VEDOVELLO, DANILA; RODRIGUES, LAURA C.; WITKIN, STEVEN S.; PASSOS, SAULO D.. Zika virus infection in pregnancy and adverse fetal outcomes in SAo Paulo State, Brazil: a prospective cohort study. SCIENTIFIC REPORTS, v. 10, n. 1, JUL 29 2020. (16/08578-0)

ANTUNES PASCALICCHIO BERTOZZI, ANA PAULA; GAZETA, ROSA ESTELA; GILLO FAJARDO, THAMIRYS COSMO; MORON, ANTONIO FERNANDES; SORIANO-ARANDES, ANTONI; ALARCON, ANA; GARCIA-ALIX, ALFREDO; DA SILVA, ALIFY BERTOLDO; FLORENCE FILHO, NEMESIO; PEREIRA SARMENTO, STEPHANNO GOMES; et al. Prevalence and diagnostic accuracy of microcephaly in a pediatric cohort in Brazil: a retrospective cross-sectional study. Jornal de Pediatria, v. 97, n. 4, p. 433-439, JUL-AUG 2021. (16/08578-0)

SANCHEZ CLEMENTE, NURIA; BRICKLEY, ELIZABETH B.; FURQUIM DE ALMEIDA, MARCIA; WITKIN, STEVEN S.; DUARTE PASSOS, SAULO; GRP, JUNDIAI ZIKA COHORT. Can Zika Virus Infection in High Risk Pregnant Women Be Differentiated on the Basis of Symptoms?. Viruses-Basel, v. 12, n. 11, NOV 2020. (16/08578-0)

SANTOS, GEOVANE R.; PINTO, CLOVIS A. L.; PRUDENTE, RAPHAEL C. S.; BEVILACQUA, ESTELA M. A. F.; WITKIN, STEVEN S.; PASSOS, SAULO D.; MORON, A. F.; VENTURA, D. F.; MASSAD, E.; ZANOTTO, P. M. A.; et al. Histopathologic Changes in Placental Tissue Associated With Vertical Transmission of Zika Virus. INTERNATIONAL JOURNAL OF GYNECOLOGICAL PATHOLOGY, v. 39, n. 2, p. 157-162, MAR 2020. (16/08578-0)

DE MORAES, AMARILIS GIARETTA; AYO, CHRISTIANE MARIA; SALA ELPIDIO, LAISE NAYANA; DE SOUZA, VICTOR HUGO; UCHIBABA YAMANAKA, ALEIA HARUMI; NOGUEIRA, MAURICIO LACERDA; PASSOS, SAULO DUARTE; BRANDAO, CINARA CASSIA; DE MATTOS, LUIZ CARLOS; DO AMARAL, GREICY CEZAR; et al. HLA-G, LILRB1 and LILRB2 Variants in Zika Virus Transmission from Mother to Child in a Population from South and Southeast of Brazil. CURRENT ISSUES IN MOLECULAR BIOLOGY, v. 44, n. 7, p. 11-pg., 2022-07-01. (16/05115-9, 16/08578-0, 16/15021-1)

VEDOVELLO, DANILA; WITKIN, STEVEN S.; BOTELHO SILVA, ANDREA CRISTINA; GILLO FAJARDO, THAMIRYS COSMO; MELLO, ALEXANDRA SIQUEIRA; ANTUNES PASCALICCHIO BERTOZZI, ANA PAULA; DA SILVA, ALIFY BERTOLDO; VIEIRA FILHO, NEMESIO FLORENCE; DUARTE RODRIGUES, MARIA MANOELA; GAZETA, ROSA ESTELA; et al. Detection of Zika virus in paired urine and amniotic fluid samples from symptomatic and asymptomatic women and their babies during a disease outbreak: association with neurological symptoms in newborns. JOURNAL OF NEUROVIROLOGY, v. 26, n. 1, p. 70-76, FEB 2020. (16/08578-0, 17/22007-8)

MELLO, ALEXANDRA SIQUEIRA; PASCALICCHIO BERTOZZI, ANA PAULA A.; RODRIGUES, MARIA MANOELA D.; GAZETA, ROSA ESTELA; MORON, ANTONIO F.; SORIANO-ARANDES, ANTONI; PEREIRA SARMENTO, STEPHANNO GOMES; VEDOVELLO, DANILA; SILVA, ANDREA CRISTINA B.; GRILLO FAJARDO, THAMIRYS C.; et al. Development of Secondary Microcephaly After Delivery: Possible Consequence of Mother-Baby Transmission of Zika Virus in Breast Milk. AMERICAN JOURNAL OF CASE REPORTS, v. 20, p. 723-725, MAY 21 2019. (16/08578-0, 17/22007-8)

COSTA ALECRIM, MARIA DAS GRACAS; RAMOS DE AMORIM, MELANIA MARIA; BARRETO DE ARAUJO, THALIA VELHO; BRASIL, PATRICIA; BRICKLEY, ELIZABETH B.; CASTILHO, MARCIA DA COSTA; COELHO, BERNADETE PEREZ; LEDO ALVES DA CUNHA, ANTONIO JOSE; DUARTE, GERALDO; ESTOFOLETE, CASSIA FERNANDA; et al. Zika Brazilian Cohorts (ZBC) Consortium: Protocol for an Individual Participant Data Meta-Analysis of Congenital Zika Syndrome after Maternal Exposure during Pregnancy. Viruses-Basel, v. 13, n. 4, APR 2021. (15/12295-0, 17/21688-1, 16/05115-9, 16/08578-0, 16/15021-1, 13/21719-3)

SANTOS, GEOVANE R.; PINTO, CLOVIS A. L.; PRUDENTE, RAPHAEL C. S.; WITKIN, STEVEN S.; ARANDES, ANTONI S.; RODRIGUES, LAURA C.; ZATZ, MAYANA; MASSAD, EDUARDO; PASSOS, SAULO D.; ZIKA CONSORTIUM. Differences in Placental Histology Between Zika Virus-infected Teenagers and Older Women. INTERNATIONAL JOURNAL OF GYNECOLOGICAL PATHOLOGY, v. 41, n. 4, p. 8-pg., 2022-07-01. (16/08578-0)

CLEMENTE, NURIA SANCHEZ; RODRIGUES, MANOELA; PASCALICCHIO, ANA PAULA; GAZETA, ROSA ESTELA; VEDOVELLO, DANILA; BRICKLEY, ELIZABETH B.; DE ALMEIDA, MARCIA F.; PASSOS, SAULO D.. Cohort profile: the Jundiai Zika cohort (JZC), a pregnancy and birth cohort in SAo Paulo state, Brazil. BMJ OPEN, v. 9, n. 8, AUG 2019. (16/08578-0)