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Application of antimicrobial peptides as new additives in animal nutrition

Abstract

During the past decades, a great number of antimicrobial peptides (AMPs) have been identified from a variety of species of vertebrates and invertebrates, such as magainin, aurein, cecropin, ceratotoxin, among others. Especially, the antimicrobial peptide Ctx(Ile21)-Ha, extracted and isolated from the skin secretion of an arboreal South American frog (Hypsiboas albopunctatus), has been studied and demonstrated to be a very promising molecule, due to the biological activities presented against fungus and bacteria. Regarding its property of bacterial membrane destruction and permeabilization, leading to death, those peptides are an interesting target for improvement for beef cattle nutrition and laying hens, which can be applied in animal nutrition. In this way, this project objective uses the AMP Ctx(Ile21)-Ha in the ruminant nutrition, in order to decrease the Gram-positive bacteria, harmful to the ruminal process, which decreases the animal nutrition efficiency by means of increasing the production of enteric methane. Thus, the project foresees, in addition to the synthesis of the Ctx(Ile21)-Ha peptide, an evaluation of the biological activity of the molecule against the undesirable microorganisms and, lately, in vitro e in vivo studies to verify the viability of these molecules in the ruminal liquor. In the same manner, this propose aims the evaluation of in vitro assays in monogastric animals, such as laying hens and swines, and the verification of zootechnical indexes to aim the production increase; also, analyze the peptide as a food preservative molecule, in order to increase the shelf-life. At the same time, this propose will perform peptide conjugated synthesis and specific structural modifications in primary sequence of the Ctx(Ile21)-Ha peptide, aiming the improvement and targeting the biological activity, which will result in compounds with potential antibiotic activity and able to be tested also as an additive in others animal species and verify its action on their production indexes. (AU)

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Scientific publications (9)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SILVEIRA, RAIZA F.; ROQUE-BORDA, CESAR A.; VICENTE, EDUARDO F. Antimicrobial peptides as a feed additive alternative to animal production, food safety and public health implications: An overview. ANIMAL NUTRITION, v. 7, n. 3, p. 896-904, SEP 2021. Web of Science Citations: 0.
TADAYOZZI, YASMIN SAEGUSA; SANTOS, FELIPE ANDRE DOS; VICENTE, EDUARDO FESTOZO; FORTI, JULIANE CRISTINA. Application of oxidative process to degrade paraquat present in the commercial herbicide. JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMIN, v. 56, n. 7, p. 670-674, AUG 3 2021. Web of Science Citations: 0.
ROQUE-BORDA, CESAR AUGUSTO; LEITE SILVA, HANYENY RAIELY; CRUSCA JUNIOR, EDSON; SERAFIM, JESSICA APARECIDA; MENEGUIN, ANDREIA BAGLIOTTI; CHORILLI, MARLUS; MACEDO, WAGNER COSTA; TEIXEIRA, SILVIO RAINHO; LOPES GUASTALLI, ELISABETE APARECIDA; SOARES, NILCE MARIA; BLAIR, JESSICA M. A.; PIKRAMENOU, ZOE; VICENTE, EDUARDO FESTOZO. Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile(21))-Ha antimicrobial peptide application as a feed additive. International Journal of Biological Macromolecules, v. 183, p. 1236-1247, JUL 31 2021. Web of Science Citations: 2.
ROQUE-BORDA, CESAR AUGUSTO; KULUS, DARIUSZ; VACARO DE SOUZA, ANGELA; KAVIANI, BEHZAD; VICENTE, EDUARDO FESTOZO. Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 22, n. 11 JUN 2021. Web of Science Citations: 0.
ROQUE-BORDA, CESAR AUGUSTO; PEREIRA, LARISSA PIRES; GUASTALLI, ELISABETE APARECIDA LOPES; SOARES, NILCE MARIA; MAC-LEAN, PRISCILLA AYLEEN BUSTOS; SALGADO, DOUGLAS D'ALESSANDRO; MENEGUIN, ANDREIA BAGLIOTTI; CHORILLI, MARLUS; VICENTE, EDUARDO FESTOZO. HPMCP-Coated Microcapsules Containing the Ctx(Ile(21))-Ha Antimicrobial Peptide Reduce the Mortality Rate Caused by Resistant Salmonella Enteritidis in Laying Hens. ANTIBIOTICS-BASEL, v. 10, n. 6 JUN 2021. Web of Science Citations: 0.
TADAYOZZI, YASMIN SAEGUSA; SANTOS, FELIPE ANDRE DOS; VICENTE, EDUARDO FESTOZO; FORTI, JULIANE CRISTINA. Application of oxidative process to degrade paraquat present in the commercial herbicide. JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMIN, JUN 2021. Web of Science Citations: 0.
SERAFIM, JESSICA APARECIDA; SILVEIRA, RAIZA FELISMINO; VICENTE, EDUARDO FESTOZO. Fast Determination of Short-Chain Fatty Acids and Glucose Simultaneously by Ultraviolet/Visible and Refraction Index Detectors via High-Performance Liquid Chromatography. FOOD ANALYTICAL METHODS, v. 14, n. 7 FEB 2021. Web of Science Citations: 0.
NICODEMO, DANIEL; MINGATTO, FABIO ERMINIO; DE JONG, DAVID; VEIGA BIZERRA, PAULO FRANCISCO; TAVARES, MARCO AURELIO; BELLINI, WILLIAM CESAR; VICENTE, EDUARDO FESTOZO; DE CARVALHO, AMANDA. Mitochondrial Respiratory Inhibition Promoted by Pyraclostrobin in Fungi is Also Observed in Honey Bees. Environmental Toxicology and Chemistry, v. 39, n. 6, p. 1267-1272, MAY 2020. Web of Science Citations: 0.
AMORIM, CLEBER A.; BLANCO, KATE C.; COSTA, IVANI M.; VICENTE, EDUARDO F.; PETRUCI, JOAO FLAVIO DA S.; CONTIERO, JONAS; LEITE, EDSON R.; CHIQUITO, ADENILSON J. Active-electrode biosensor of SnO2 nanowire for cyclodextrin detection from microbial enzyme. Nanotechnology, v. 31, n. 16 APR 17 2020. Web of Science Citations: 0.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.
Filed patent(s) as a result of this research project

Solicitação em análise e dentro do prazo legal de sigilo previsto na legislação BR1020180687638 - Universidade de São Paulo (USP) . Solicitação em análise e dentro do prazo legal de sigilo previsto na legislação - September 2018, 14