Integrated analysis of quantitative genetics and genomics to select genotypes of pacu (Piaractus mesopotamicus)

• Grant number: 16/21011-9
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2017
• End date: April 30, 2019
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Diogo Teruo Hashimoto
• Grantee: Diogo Teruo Hashimoto
• Host Institution: Centro de Aquicultura (CAUNESP). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• City of the host institution: Jaboticabal
• Associated researchers: Fabiana Pilarski ; Rafael Vilhena Reis Neto ; Roberto Carvalheiro

Abstract

The species Piaractus mesopotamicus (pacu) has occurrence in the basins of the Parana-Paraguay River and corresponds to one of the most fish produced in Brazilian aquaculture. Therefore, it is one of the main fish intended for breeding programs, especially because of its market acceptance (national and international). The objective of this project is to evaluate the performance of pacu families regarding the growth characteristics and resistance to Aeromonas hydrophila, and characterize QTLs (Quantitative Trait Locus) through gene mapping with SNPs (Single Nucleotide Polymorphisms). In total, 45 families of pacu will be done in the breeding program, which will be assessed the quantitative genetic parameters of five growth measurements (weight and four morphometric characteristics), and the survival rate of the bacterial challenge with Aeromonas hydrophila. In relation to the characterization of QTLs, 10 families of pacu will be selected for evaluation of each characteristic (growth and resistance). Then, sequencing/genotyping of SNPs will be performed by ddRAD-seq (double digest restriction site associated DNA sequencing), with the enzymes SphI and MluCI. In addition to the introduction of new genetic technologies for the aquaculture industry, the perspective is that the information of the SNPs will allow the construction of high density genetic maps, which will optimize the selection of superior genotypes for genetic improvement and increase the productivity of this important fish group. (AU)

Scientific publications

(8)

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

MASTROCHIRICO-FILHO, VITO A.; ARIEDE, RAQUEL B.; FREITAS, V, MILENA; LIRA, LIESCHEN V. G.; AGUDELO, JOHN F. G.; PILARSKI, FABIANA; REIS NETO, V, RAFAEL; YANEZ, JOSE M.; HASHIMOTO, DIOGO T.. Genetic parameters for resistance to Aeromonas hydrophila in the Neotropical fish pacu (Piaractus mesopotamicus). Aquaculture, v. 513, NOV 15 2019. (16/18294-9, 16/21011-9)

MASCALI, FLORENCIA CARLA; MASTROCHIRICO-FILHO, VITO ANTONIO; POSNER, VICTORIA MARIA; RUBIOLO, JUAN ANDRES; HASHIMOTO, DIOGO TERUO; VILLANOVA, GABRIELA VANINA. High-quality genome assembly and annotation of the pacu Piaractus mesopotamicus: an aquatic genetic resource of South American aquaculture. AQUACULTURE INTERNATIONAL, v. 32, n. 7, p. 23-pg., 2024-07-09. (18/08416-5, 16/21011-9, 22/09226-0, 20/11049-4)

FREITAS, V, MILENA; LIRA, LIESCHEN V. G.; ARIEDE, RAQUEL B.; AGUDELO, JOHN F. G.; DE OLIVEIRA NETO, RUBENS RICARDO; BORGES, CAROLINA H. S.; MASTROCHIRICO-FILHO, VITO A.; GARCIA NETO, BALTASAR F.; CARVALHEIRO, ROBERTO; HASHIMOTO, DIOGO T.. Genotype by environment interaction and genetic parameters for growth traits in the Neotropical fish pacu (Piaractus mesopotamicus). Aquaculture, v. 530, JAN 15 2021. (16/21011-9)

MASTROCHIRICO-FILHO, VITO A.; BORGES, CAROLINA H. S.; FREITAS, MILENA V.; ARIEDE, RAQUEL B.; PILARSKI, FABIANA; UTSUNOMIA, RICARDO; CARVALHEIRO, ROBERTO; GUTIERREZ, ALEJANDRO P.; PENALOZA, CAROLINA; YANEZ, JOSE M.; et al. Development of a SNP linkage map and genome-wide association study for resistance to Aeromonas hydrophila in pacu (Piaractus mesopotamicus). BMC Genomics, v. 21, n. 1, SEP 29 2020. (18/08416-5, 16/21011-9, 17/26900-9, 16/18294-9)

MASTROCHIRICO-FILHO, VITO ANTONIO; HATA, MILENE ELISSA; KURADOMI, RAFAEL YUTAKA; DE FREITAS, MILENA VIEIRA; ARIEDE, RAQUEL BELINI; PINHEIRO, DANIEL GUARIZ; ROBLEDO, DIEGO; HOUSTON, ROSS; HASHIMOTO, DIOGO TERUO. Transcriptome Profiling of Pacu (Piaractus mesopotamicus) Challenged With PathogenicAeromonas hydrophila: Inference on Immune Gene Response. FRONTIERS IN GENETICS, v. 11, JUN 9 2020. (16/21011-9, 17/26900-9)

FREITAS, MILENA, V; LEMOS, CELMA G.; ARIEDE, RAQUEL B.; AGUDELO, JOHN F. G.; NETO, RUBENS R. O.; BORGES, CAROLINA H. S.; MASTROCHIRICO-FILHO, VITO A.; PORTO-FORESTI, FABIO; IOPE, ROGERIO L.; BATISTA, FABRICIO M.; et al. High-throughput phenotyping by deep learning to include body shape in the breeding program of pacu (Piaractus mesopotamicus). Aquaculture, v. 562, p. 8-pg., 2023-01-15. (16/21011-9)

MASTROCHIRICO-FILHO, VITO A.; ARIEDE, RAQUEL B.; FREITAS, V, MILENA; BORGES, CAROLINA H. S.; LIRA, LIESCHEN V. G.; MENDES, NATALIA J.; AGUDELO, JOHN F. G.; CACERES, PABLO; BERROCAL, MILTHON H. M.; SUCERQUIA, GUSTAVO A. L.; et al. Development of a multi-species SNP array for serrasalmid fish Colossoma macropomum and Piaractus mesopotamicus. SCIENTIFIC REPORTS, v. 11, n. 1, SEP 29 2021. (16/21011-9, 17/26900-9, 20/11049-4, 16/18294-9, 18/08416-5, 17/19717-3, 19/10662-7, 19/08972-8)

GOMEZ AGUDELO, JOHN FREDY; MASTROCHIRICO-FILHO, VITO ANTONIO; DE SOUZA BORGES, CAROLINA HELOISA; ARIEDE, RAQUEL BELINI; GUERRA LIRA, LIESCHEN VALERIA; DE OLIVEIRA NETO, RUBENS RICARDO; FREITAS, MILENA VIEIRA; LENIS SUCERQUIA, GUSTAVO ADOLFO; VERA, MANUEL; MUNOZ BERROCAL, MILTHON HONORIO; et al. Genomic selection signatures in farmed Colossoma macropomum from tropical and subtropical regions in South America. EVOLUTIONARY APPLICATIONS, v. 15, n. 4, p. 15-pg., 2022-02-24. (16/21011-9)