Advanced search
Start date
Betweenand

Investigation of the pathophysiological aspects and novel therapeutic approaches for thromboembolic disorders

Grant number: 16/14172-6
Support type:Research Projects - Thematic Grants
Duration: May 01, 2017 - October 31, 2022
Field of knowledge:Health Sciences - Medicine - Medical Clinics
Principal researcher:Joyce Maria Annichino-Bizzacchi
Grantee:Joyce Maria Annichino-Bizzacchi
Home Institution: Centro de Hematologia e Hemoterapia (HEMOCENTRO). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Pesquisadores principais:
Erich Vinicius de Paula ; Fernanda Loureiro de Andrade Orsi ; Katia Borgia Barbosa Pagnano
Assoc. researchers: Beatriz de Moraes Martinelli ; Bruna de Moraes Mazetto Fonseca ; Carmen Silvia Passos Lima ; Carolina Mie Kawagosi Onodera ; Claudio Alberto Torres Suazo ; Fábio Hüsemann Menezes ; Fernanda Dutra Santiago Bassora ; Gerson Jhonatan Rodrigues ; Isabella Macedo Toni ; José Barreto Campello Carvalheira ; Kiara Cristina Senger Zapponi Cerri ; Letícia Queiroz da Silva ; Ljubica Tasic ; Luis Fernando Bittar Sckayer ; Maurício Etchebehere ; Melissa Quintero Escobar ; Nelson Adami Andreollo ; Nelson Wolosker ; Patricia Moriel ; Rubens Maciel Filho ; Silmara Aparecida de Lima Montalvão ; Simone Appenzeller ; Stephany Cares Huber ; Suely Meireles Rezende ; Tayana Bezerra Teixeira Mello ; Tiago Dias Martins ; Vitória Régia Pereira Pinheiro ; Wagner José Fávaro
Associated grant(s):20/05985-9 - Evaluation of the mechanisms of hemostatic activation in COVID-19 and their modulation by bradykinin inhibitors, AP.R
19/23552-5 - Vasoreparative processes in the pathogenesis of deep vein thrombosis (DVT), AP.R SPRINT
18/02589-5 - EMU granted in process 2016 / 14172-6 - 02 gene rotors Q5plex-HRM platform - Quiagen brand, AP.EMU
17/15021-4 - Multi user equipament granted in process 2016/14172-6: automated optical microscopy for phase contrast system and dual color fluorescence, model incucyte zoom HD/2 channel, Essen Bioscience (exclusivity), AP.EMU
Associated scholarship(s):22/05399-8 - ASSESSMENT OF RISK FACTORS FOR DEEP VENOUS THROMBOSIS IN CANCER PATIENTS., BP.TT
22/06186-8 - EPIDEMIOLOGY OF VENOUS THROMBOEMBOLISM IN CHILDHOOD AND ADOLESCENCE: DISEASE REGISTRATION IN A BRAZILIAN POPULATION., BP.TT
21/12945-6 - Exploration of tissue injury mechanisms in Sepsis in patients with Febrile Neutropenia, BP.PD
+ associated scholarships 22/04921-2 - ASSESSMENT OF RISK FACTORS FOR DEEP VENOUS THROMBOSIS IN CANCER PATIENTS., BP.TT
22/05164-0 - Evaluation of risk factors for venous thromboembolism in cancer patients., BP.TT
21/14607-0 - Assessment of drug interactions with warfarin in patients diagnosed with Primary Antiphospholipid Syndrome, BP.IC
22/00902-3 - Evaluation of the activity of circulating colony forming endothelial cells (ECFCs) in patients with spontaneous and induced venous thrombosis., BP.TT
21/10715-3 - New methodologies for assessing coagulation activation and endothelial barrier integrity, BP.TT
21/09984-0 - Assessment of risk factors for deep venous thrombosis in cancer patients, BP.TT
21/07212-0 - Metabolomic and metabonomic analyzes by nuclear magnetic resonance techniques applied in studies of Venous Thrombosis, BP.PD
21/02831-3 - Epidemiology of venous thromboembolism in childhood and adolescence: disease registration in a Brazilian population, BP.TT
21/02824-7 - ASSESSMENT OF RISK FACTORS FOR DEEP VENOUS THROMBOSIS IN CANCER PATIENTS., BP.TT
21/02830-7 - Evaluation of the activity of circulating colony forming endothelial cells (ECFCs) in patients with spontaneous and induced venous thrombosis., BP.TT
20/16105-0 - Characterization of the thromboembolic risk in COVID-19, BP.IC
20/15499-4 - Epidemiology of venous thromboembolism in childhood and adolescence: disease registration in a Brazilian population, BP.TT
19/20136-0 - Evaluation of signature of genes related to thromboty diseases in antiphospholipid syndrome, BP.MS
20/06156-6 - Importance of antiphospholipid antibodies of the IgA class for the prognosis of antiphospholipid syndrome, BP.IC
20/08990-3 - Assessment of risk factors for deep venous thrombosis in cancer patients, BP.TT
19/20320-6 - Role of extracellular vesicles as markers of inflammation and adverse clinical outcomes in orthotopic liver transplantation, BP.MS
19/20891-3 - Characterization of plasmacytoid dendritic cell activity and type 1 interferon signature in primary antiphospholipid syndrome with thrombosis, BP.MS
19/18195-9 - New methodologies for assessing coagulation activation and endothelial barrier integrity, BP.TT
19/06318-9 - Thrombosis and Inflammation: Participation of innate immunity and NETosis in the pathogenesis of hypercoagulability in chronic myeloproliferative, BP.TT
18/15618-3 - Evaluation of the activity of circulating colony forming endothelial cells (ECFCs) in patients with spontaneous and induced Venous Thrombosis, BP.DR
18/24577-9 - The role of tissue factor in APS hypercoagulability, BP.TT
18/18624-4 - Metabolomic and metabonomic analyzes by nuclear magnetic resonance techniques applied in studies of venous thrombosis, BP.PD
18/20440-9 - Evaluation of risk factors for venous thromboembolism in cancer patients, BP.TT
18/10602-1 - Preditive factors of recurrent venous thromboembolism using design of experiments, BP.IC
18/14349-9 - Thrombosis and inflammation: participation of innate immunity and NETosis in the pathogenesis of hypercoagulability in chronic myeloproliferative diseases, BP.TT
18/11308-0 - The role of tissue factor in hypercoagulability of the SAF, BP.TT
17/19251-4 - MicroRNAs and oxidized biomolecules as possible biomarkers of primary antiphospholipid syndrome, BP.MS - associated scholarships

Abstract

Thromboembolic disorders represent a major culprit for morbidity-mortality in Brazil and the World. The Hemocentro at Unicamp has a scientifically recognized research group, with over 100 highly cited publications in this field. We have revealed the roles of FVIII, VWF and of ADAMTS13 in Deep Venous Thrombosis (DVT) and severe Post-Thrombotic Syndrome (PTS); the association between hypercoagulability, inflammation and neutrophils; circulating endothelial cells and repairing processes; protein expression in venous thrombus in animals exposed arterial risk factors; platelet proteins differentially expressed and in vitro studies supporting these results. Analyze the images of residual thrombi using the Grayscale Median (GSM) suggested clinical applicability for rethrombosis. We have demonstrated immature platelet fraction and generation of thrombin in acute inflammatory process such as sepsis and SIRS. We investigated gene therapy using VEGF in peripheral arterial occlusive model in rats, and we are evaluating fat stromal cells or Platelet Rich Plasma (PRP) in murine models. All these studies demonstrated that many issues remain open and the continuity of our investigations. Hemocentro at Unicamp has a bioterium with models for arterial and vein thrombosis, imaging methods like laser doppler perfusion imager and doppler ultrasound, and transgenic models available at the animal facility center of the University, CEMIB. Our outpatient clinic has over 500 patients followed for 5 years, and patients diagnosed with DVT at the Unicamp Hospital School are currently referred to our service. Patients with Antiphospholipid Syndrome and thrombotic manifestations constitute a fruitful cohort for investigation. In this thematic project, the focus will be maintained on the pathophysiological mechanisms in DVT and investigation of novel therapies to the prevention of atherosclerosis and treatment of peripheral arterial disease. The collaboration established with the professors of renowned research groups (Paolo Simmioni, University of Padua, Italy; Leonardo Brandão, Hospital for Sick Children, Toronto, Canadá; Ricardo Forastieiro, Favaloro University, Buenos Aires, Argentina; Jose Diaz, University of Michigan, Ann Arbor, EUA) will be of great support for the development of cutting-edge research. Our research strategy will be divided into five main branches: 1) DVT - Evaluate Neutrophil Extracellular Traps (NETs), hypercoagulability and inflammation correlating them with the development of PTS and recurrence. Analyze prospectively residual thrombi using GSM and validate their association with rethrombosis. Determine DVT prevalence in a pediatric registry. Analyse mathematically DVT recurrence using artificial neural nets and fuzzy logic. Investigate the endothelium contribution to DVT development; 2) Cancer - Explore NETs in patients with Polycythemia Vera and Essential Thrombocythemia. Evaluate the mechanisms associated to cardiovascular events in patients with Chronic Myeloid Leukemia treated with tyrosine kinase inhibitor. Investigate in a Brazilian multicenter study DVT risk factors in cancer patients; 3) Antiphospholipid Syndrome - Evaluate the pathophysiology of thrombotic events, risk factors and novel therapeutic approaches; 4) Inflammation and thrombosis - explore the association between inflammation and hemostasis activation, focusing the interfaces between innate immune system and hypercoagulability of acute and chronic disorders; 5) Novel therapeutic approaches - evaluate PRP upon angiogenesis in patients with Peripheral Arterial Occlusive Disease (PAOD); PRP and Mesenchymal Cells (MSCs), in the healing process of ulcers in animal models; the use of low doses of rivaroxaban and dabigatran and development of atherosclerosis in LDL or apoE deficient murine model. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications (25)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MARTINS, T. D.; ANNICHINO-BIZZACCHI, J. M.; ROMANO, A. V. C.; MACIEL FILHO, R.. Artificial neural networks for prediction of recurrent venous thromboembolism. INTERNATIONAL JOURNAL OF MEDICAL INFORMATICS, v. 141, . (16/14172-6)
FERREIRA, TATIANE; HUBER, STEPHANY CARES; MARTINELLI, BEATRIZ DE MORAES; LEMOS JUNIOR, ARLINDO; MENEZES, FABIO HUSEMANN; ORSI, FERNANDA ANDRADE; BITTAR, LUIS FERNANDO; GERBASE DE OLIVEIRA, LUIZ FREDERICO; SODRE, LEONARDO RAGAZZI; MELLO, TAYANA TEIXEIRA; et al. Low prevalence of Post-thrombotic syndrome in patients treated with rivaroxaban. VASCULAR PHARMACOLOGY, v. 124, . (16/14172-6)
VAZ, CAMILA O.; MAZETTO, BRUNA M.; VASCONCELOS, PEDRO EDUARDO N. S.; BASTOS, LARISSA B.; CURSINO, MARIA APARECIDA; FRANCA QUINTANILHA, JULIA COELHO; TRIPIQUIA VECHIATTO MESQUITA, GABRIELA LISIANE; SANTOS, ANA PAULA R.; JACINTHO, BRUNA CARDOSO; OLIVEIRA, JOSE DIOGO; et al. Association between plasmatic oxidative stress and thrombosis in primary antiphospholipid syndrome. JOURNAL OF THROMBOSIS AND THROMBOLYSIS, v. 52, n. 3, p. 730-737, . (16/14172-6)
DA SILVA, LETICIA QUEIROZ; CANCELA, REBECA BRITO BONANI; DE LIMA MONTALVAO, SILMARA APARECIDA; HUBER, STEPHANY CARES; VIEIRA-DAMIANI, GISLAINE; TRIGLIA, RENATA MARCHI; ANNICHINO-BIZZACCHI, JOYCE MARIA. The effect of lyophilized platelet rich-plasma on skin aging: a non-randomized, controlled, pilot trial. ARCHIVES OF DERMATOLOGICAL RESEARCH, v. 313, n. 10, p. 863-871, . (16/14172-6)
MAZETTO, BRUNA DE MORAES; LAZARINI, MARIANA; TOBALDINI, LAIS QUINTEIRO; ARANTES, FERNANDA TALGE; DOS SANTOS, ANA PAULA ROSA; JACINTO, BRUNA CARDOSO; VAZ, CAMILA DE OLIVEIRA; MESQUITA, GABRIELA TRIPIQUIA VECHIATTO; SARAIVA, SABRINA DA SILVA; ANNICHINO-BIZZACCHI, JOYCE; et al. Expression of tissue factor mRNA in thrombosis associated with antiphospholipid syndrome. JOURNAL OF THROMBOSIS AND THROMBOLYSIS, v. 51, n. 2, . (16/14172-6)
RODRIGUES, THAYS C. R.; DE OLIVEIRA VAZ, CAMILA; MIRANDA, ELIANA C. M.; PEREIRA, MARCOS; DA SILVA SARAIVA, SABRINA; ANNICHINO-BIZZACCHI, JOYCE MARIA; DE MORAES MAZETTO, BRUNA; ORSI, FERNANDA A.. fficacy of a hypolipid diet in patients with primary antiphospholipid syndrome with dyslipidemia: a prospective stud. JOURNAL OF THROMBOSIS AND THROMBOLYSIS, v. 53, n. 2, . (16/14172-6)
BITTAR, LUIS FERNANDO; DA SILVA, LETICIA QUEIROZ; DE ANDRADE ORSI, FERNANDA LOUREIRO; SENGER ZAPPONI, KIARA CRISTINA; MAZETTO, BRUNA DE MORAES; DE PAULA, ERICH VINICIUS; DE LIMA MONTALVAO, SILMARA APARECIDA; ANNICHINO-BIZZACCHI, JOYCE MARIA. Increased inflammation and endothelial markers in patients with late severe post-thrombotic syndrome. PLoS One, v. 15, n. 1, . (16/14172-6)
LADEIRA CRUZ, ANA CLARA; COLELLA, MARINA PEREIRA; DE PAULA, ERICH VINICIUS; ANNICHINNO-BIZZACHI, JOYCE; ORSI, FERNANDA ANDRADE. Clinical course of primary immune thrombocytopenia with positive antiphospholipid antibodies. EUROPEAN JOURNAL OF INTERNAL MEDICINE, v. 69, p. E6-E7, . (16/14172-6)
LADEIRA CRUZ, ANA CLARA; COLELLA, MARINA PEREIRA; DE PAULA, ERICH VINICIUS; ANNICHINNO-BIZZACHI, JOYCE; ORSI, FERNANDA ANDRADE. Clinical course of primary immune thrombocytopenia with positive antiphospholipid antibodies: Author's reply. EUROPEAN JOURNAL OF INTERNAL MEDICINE, v. 74, p. 108-109, . (16/14172-6)
NOBREGA BARBOSA, AYLA CRISTINA; LIMA MONTALVAO, SILMARA APARECIDA; NOBREGA BARBOSA, KEVAN GUILHERME; COLELLA, MARINA PEREIRA; ANNICHINO-BIZZACCHI, JOYCE MARIA; OZELO, MARGARETH CASTRO; DE PAULA, ERICH VINICIUS. Prolonged APTT of unknown etiology: A systematic evaluation of causes and laboratory resource use in an outpatient hemostasis academic unit. RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS, v. 3, n. 4, p. 749-757, . (16/14172-6)
SARAIVA-MANGOLIN, SABRINA; VAZ, CAMILA DE OLIVEIRA; RUIZ, THAYS; MAZETTO, BRUNA MORAES; ORSI, FERNANDA ANDRADE. Use of hydroxychloroquine to control immune response and hypercoagulability in patients with primary antiphospholipid syndrome. EUROPEAN JOURNAL OF INTERNAL MEDICINE, v. 90, p. 114-115, . (16/14172-6)
MARTINS, TIAGO DIAS; FILHO, RUBENS MACIEL; ROMANO, ANNA VIRGINIA CALAZANS; ANNICHINO-BIZZACCHI, JOYCE MARIA. Comment and Update on ``Using Artificial Intelligence to Manage Thrombosis Research, Diagnosis, and Clinical Management{''}. SEMINARS IN THROMBOSIS AND HEMOSTASIS, v. 47, n. 01, p. 112-114, . (16/14172-6)
VAZ, CAMILA DE OLIVEIRA; MAZETTO FONSECA, BRUNA DE MORAES; NASCIMENTO SILVA VASCONCELOS, PEDRO EDUARDO; BASTOS, LARISSA BRITO; CURSINO, MARIA APARECIDA; FRANCA QUINTANILHA, JULIA COELHO; TRIPIQUIA VECHIATTO MESQUITA, GABRIELA LISIANE; ROSA DOS SANTOS, ANA PAULA; JACINTHO, BRUNA CARDOSO; OLIVEIRA, JOSE DIOGO; et al. Self-perceived quality of life of primary antiphospholipid syndrome patients using vitamin K antagonist. Lupus, v. 30, n. 5, p. 707-714, . (16/14172-6)
MARTINS, TIAGO D.; ANNICHINO-BIZZACCHI, JOYCE M.; ROMANO, ANNA V. C.; MACIEL FILHO, RUBENS. Principal Component Analysis on Recurrent Venous Thromboembolism. CLINICAL AND APPLIED THROMBOSIS-HEMOSTASIS, v. 25, . (16/14172-6)
HOUNKPE, BIDOSSESSI WILFRIED; CHENOU, FRANCINE; DOMINGOS, IGOR DE FARIAS; CARDOSO, EVILAZIO CUNHA; COSTA SOBREIRA, MARCONDES JOSE DE VASCONCELOS; ARAUJO, ADERSON S.; LUCENA-ARAUJO, ANTONIO ROBERTO; DA SILVA NETO, PEDRO VIEIRA; MALHEIRO, ADRIANA; FRAIJI, NELSON ABRAHIM; et al. Neutrophil extracellular trap regulators in sickle cell disease: Modulation of gene expression of PADI4, neutrophil elastase, and myeloperoxidase during vaso-occlusive crisis. RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS, v. 5, n. 1, . (14/00984-3, 15/24666-3, 16/14172-6)
ESCOBAR, MELISSA QUINTERO; TASIC, LJUBICA; COSTA, TASSIA BRENA BARROSO CARNEIRO DA; STANISIC, DANIJELA; MONTALVAO, SILMARA; HUBER, STEPHANY; ANNICHINO-BIZZACCHI, JOYCE MARIA. Serum Metabolic Profiles Based on Nuclear Magnetic Resonance Spectroscopy among Patients with Deep Vein Thrombosis and Healthy Controls. METABOLITES, v. 11, n. 12, . (18/24069-3, 16/14172-6, 18/18624-4)
ZAPPONI, KIARA C. S.; ORSI, FERNANDA A.; CUNHA, JOSE LUIZ R.; DE BRITO, INGRID R.; ROMANO, ANNA VIRGINIA C.; BITTAR, LUIS FERNANDO; DE PAULA, ERICH VINICIUS; PENTEADO, CARLA F.; MONTALVAO, SILMARA; ANNICHINO-BIZZACCHI, JOYCE MARIA. Neutrophil activation and circulating neutrophil extracellular traps are increased in venous thromboembolism patients for at least one year after the clinical event. JOURNAL OF THROMBOSIS AND THROMBOLYSIS, v. 53, n. 1, . (16/14172-6, 13/22981-3, 13/26244-3)
GOMES SANTATERRA, VANESSA ARAUJO; LUZ FIUSA, MAIARA MARX; HOUNKPE, BIDOSSESSI WILFRIED; CHENOU, FRANCINE; TONASSE, WOUITCHEKPO VINCENT; GOMES DA COSTA, LOREDANA NILKENES; GARCIA-WEBER, DIEGO; DOMINGOS, IGOR DE FARIAS; DE LIMA, FRANCIELE; BORBA-JUNIOR, IVANIO TEIXEIRA; et al. Endothelial Barrier Integrity Is Disrupted In Vitro by Heme and by Serum From Sickle Cell Disease Patients. FRONTIERS IN IMMUNOLOGY, v. 11, . (14/00984-3, 16/14172-6)
HUBER, STEPHANY CARES; MARTINELLI, BEATRIZ DE MORAES; QUINTERO, MELISSA; SILVA DE PAULA, LAIS IVINA; CATALDO, JOSE LUIZ; DE LIMA MONTALVAO, SILMARA APARECIDA; ANNICHINO-BIZZACCHI, JOYCE M.. A case series of platelet rich plasma in chronic venous ulcers. REGENERATIVE THERAPY, v. 18, p. 51-58, . (18/18624-4, 16/14172-6)
DE SOUZA, JULIA RUETE; YOKOYAMA, ANA PAULA; MAGNUS, MARIANA MUNARI; BOIN, ILKA; DE ATAIDE, ELAINE CRISTINA; MUNHOZ, DERLI CONCEICAO; PEREIRA, FABRICIO BISCARO; LUZO, ANGELA; ORSI, FERNANDA ANDRADE. Association of acidosis with coagulopathy and transfusion requirements in liver transplantation. JOURNAL OF THROMBOSIS AND THROMBOLYSIS, . (16/14172-6, 18/24124-4)
HOUNKPE, BIDOSSESSI WILFRIED; CHENOU, FRANCINE; DE LIMA, FRANCIELE; DE PAULA, ERICH VINICIUS. HRT Atlas v1.0 database: redefining human and mouse housekeeping genes and candidate reference transcripts by mining massive RNA-seq datasets. Nucleic Acids Research, v. 49, n. D1, p. D947-D955, . (15/24666-3, 16/14172-6)
ARANTES, FERNANDA T.; MAZETTO, BRUNA M.; SARAIVA, SABRINA S.; TOBALDINI, LAIS Q.; DOS SANTOS, ANA PAULA R.; ANNICHINO-BIZZACCHI, JOYCE; ORSI, FERNANDA A.. Inflammatory markers in thrombosis associated with primary antiphospholipid syndrome. JOURNAL OF THROMBOSIS AND THROMBOLYSIS, . (16/14172-6)
COLELLA, MARINA P.; ORSI, FERNANDA A.; ALVES, ELIZIO C. F.; DELMORO, GABRIELA DE F.; YAMAGUTI-HAYAKAWA, GABRIELA G.; DE PAULA, V, ERICH; ANNICHINO-BIZZACCHI, JOYCE M.. A retrospective analysis of 122 immune thrombocytopenia patients treated with dapsone: Efficacy, safety and factors associated with treatment response. Journal of Thrombosis and Haemostasis, v. 19, n. 9, . (16/14172-6)
TOBALDINI, LAIS QUINTEIRO; ARANTES, FERNANDA TALGE; SARAIVA, SABRINA DA SILVA; MAZETTO, BRUNA DE MORAES; COLELLA, MARINA PEREIRA; DE PAULA, ERICH VINICIUS; ANNICHINO-BIZZACHI, JOYCE; ORSI, FERNANDA ANDRADE. Circulating levels of tissue factor and the risk of thrombosis associated with antiphospholipid syndrome. THROMBOSIS RESEARCH, v. 171, p. 114-120, . (16/14172-6)
CAMPOS NOGUEIRA, YZABELLA ALVES; GOMES DA COSTA, LOREDANA NILKENES; LEVY, CARLOS EMILIO; ORSI, FERNANDA ANDRADE; DE LIMA, FRANCIELE; ANNICHINNO-BIZZACCHI, JOYCE M.; DE PAULA, ERICH VINICIUS. Hypofibrinolysis induced by tranexamic acid does not influence inflammation and mortality in a polymicrobial sepsis model. PLoS One, v. 14, n. 12, . (16/14172-6)

Please report errors in scientific publications list by writing to: cdi@fapesp.br.