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Evaluation of physical, chemical and antibacterial of a novel high-plasticity repair cement


The aim of the study is to evaluate the physical, chemical and antimicrobial properties of high plasticity calcium silicate-based cements (MTA HP, Biodentine and MTA Flow) and to purpose a novel high plasticity cement composed of tricalcium and dicalcium silicate, bismuth oxide, zinc oxide and in the liquid, distilled water and water-soluble polymer. The association of zinc oxide in MTA Flow will be also evaluated to inhibit dental discoloration verified in a pilot test. The chemical characterization, bioactivity, radiopacity, setting time, flowability, volumetric change, pH, calcium release, dental discoloration and antimicrobial activity will be evaluated. Chemical characterization and bioactivity will be evaluated using SEM, EDS and XRD. Radiopacity, setting time and flowability will be determined according to ISO 6876/2001. Flowability will be also tested using volumetric method. Volumetric change will be tested using micro-CT. The pH and ion release will be evaluated after 3, 24 hours and 28 days. Dental discoloration analysis will be performed before cement insertion, immediately after insertion and after 28, 60, 90 and 180 days. Antimicrobial activity will be evaluated using inhibition test. Discoloration will be evaluated immediately after filling and 28, 60 and 90 days after filling. The results will be submitted to normality test of D'Agostino and Pearson to select the most adequate statistical test (p < 0.05). (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PELEPENKO, LAUTER E.; SAAVEDRA, FLAVIA; ANTUNES, THIAGO B. M.; BOMBARDA, GABRIELA F.; GOMES, BRENDA P. F. A.; ZAIA, ALEXANDRE A.; CAMILLERI, JOSETTE; MARCIANO, MARINA A. Physicochemical, antimicrobial, and biological properties of White-MTAFlow. CLINICAL ORAL INVESTIGATIONS, v. 25, n. 2 AUG 2020. Web of Science Citations: 0.
LAUTER ESTON PELEPENKO; FLÁVIA SAAVEDRA; GABRIELA FERNANDA BOMBARDA; BRENDA PAULA FIGUEIREDO DE ALMEIDA GOMES; ADRIANA DE-JESUS-SOARES; ALEXANDRE AUGUSTO ZAIA; MARCO ANTONIO HUNGARO DUARTE; MARIO TANOMARU-FILHO; MARINA ANGÉLICA MARCIANO. Dental discoloration caused by Grey-MTAFlow cement: analysis of its physicochemical, biological and antimicrobial properties. Journal of Applied Oral Science, v. 28, p. -, 2020. Web of Science Citations: 0.
MARCIANO, MARINA ANGELICA; CAMILLERI, JOSETTE; LUCATELI, RIBAMAR LAZANHA; COSTA, REGINALDO MENDONCA; MATSUMOTO, MARIZA AKEMI; HUNGARO DUARTE, MARCO ANTONIO. Physical, chemical, and biological properties of white MTA with additions of AlF3. CLINICAL ORAL INVESTIGATIONS, v. 23, n. 1, p. 33-41, JAN 2019. Web of Science Citations: 2.

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