Abstract
Brazil is one of the countries with the greater diversity in terms of lignocellulosic biomass. The lignocellulolytic enzymes production by filamentous fungi has been the constant subject of study, mainly because such enzymes are of crucial importance in the processes of generation biofuels from plant biomass. However, no micro-organism considered to be highly effective for the hydrolysis of biomass was yet achieved. This fact is due to the lack of a solid knowledge in regulating of the production of these enzymes. One of the main mechanisms of regulation of protein synthesis occurs by activation of phosphorylation cascades that lead to the activation of transcription factors. Thus, the understanding the signaling pathways activated in certain conditions can provide knowledge enabling the engineering the microorganism to becomes a better producer of lignocellulolytic enzymes, even in conditions with high concentrations of glucose in catabolic repression. Thus, the objectives of this project are: (1) Elucidation of the cell signaling pathways mediated by protein kinase A (PKA), MAPK and calcium, (2) phosphoproteomic identification of potential targets for phosphorylation by MAPK, PKA. (3) study of functional genomics of genes that are part of the signaling pathways by PKA, MAPK and calcium transporter identified in T. reesei. (4) to purify and characterize the secretory vesicles of T. reesei induced by sugarcane bagasse. (5) determine and identify the protein content of extracellular vesicles of T. reesei. At the end of the project we intend to contribute to the greater understanding of the biology of this important industrial fungus, producing mutant strains to increase the production of hollocelulases and lower the cost of production of enzyme cocktails that still constitute a large proportion of biotechnological products obtained from T. reesei, mainly in the production of bioethanol. (AU)
Scientific publications
(7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RIBEIRO, MARCELA SURIANI;
DE PAULA, RENATO GRACIANO;
VOLTAN, ALINE RAQUEL;
DE CASTRO, RAPHAELA GEORG;
CARRARO, CLAUDIA BATISTA;
DE ASSIS, LEANDRO JOSE;
STEINDORFF, ANDREI STECCA;
GOLDMAN, GUSTAVO HENRIQUE;
SILVA, ROBERTO NASCIMENTO;
ULHOA, CIRANO JOSE;
MONTEIRO, VALDIRENE NEVES.
Endo-beta-1,3-glucanase (GH16 Family) from Trichoderma harzianum Participates in Cell Wall Biogenesis but Is Not Essential for Antagonism Against Plant Pathogens.
BIOMOLECULES,
v. 9,
n. 12
DEC 2019.
Web of Science Citations: 2.
DE PAULA, RENATO GRACIANO;
CAMPOS ANTONIETO, AMANDA CRISTINA;
COSTA RIBEIRO, LILIANE FRAGA;
SRIVASTAVA, NEHA;
O'DONOVAN, ANTHONIA;
MISHRA, P. K.;
GUPTA, VIJAI K.;
SILVA, ROBERTO N.
Engineered microbial host selection for value-added bioproducts from lignocellulose.
BIOTECHNOLOGY ADVANCES,
v. 37,
n. 6
NOV 1 2019.
Web of Science Citations: 2.
SRIVASTAVA, NEHA;
SRIVASTAVA, MANISH;
MALHOTRA, BANSI D.;
GUPTA, VIJAI K.;
RAMTEKE, P. W.;
SILVA, ROBERTO N.;
SHUKLA, PRATYOOSH;
DUBEY, KASHYAP KUMAR;
MISHRA, P. K.
Nanoengineered cellulosic biohydrogen production via dark fermentation: A novel approach.
BIOTECHNOLOGY ADVANCES,
v. 37,
n. 6
NOV 1 2019.
Web of Science Citations: 2.
SILVA, ROBERTO N.;
MONTEIRO, VALDIRENE NEVES;
STEINDORFF, ANDREI STECCA;
GOMES, ERISTON VIEIRA;
NORONHA, ELIANE FERREIRA;
ULHOA, CIRANO J.
Trichoderma/pathogen/plant interaction in pre-harvest food security.
FUNGAL BIOLOGY,
v. 123,
n. 8,
p. 565-583,
AUG 2019.
Web of Science Citations: 1.
DE PAULA, RENATO GRACIANO;
CAMPOS ANTONIETO, AMANDA CRISTINA;
VIEIRA NOGUEIRA, KAROLINE MARIA;
COSTA RIBEIRO, LILIANE FRAGA;
ROCHA, MARINA CAMPOS;
MALAVAZI, IRAN;
ALMEIDA, FAUSTO;
SILVA, ROBERTO NASCIMENTO.
Extracellular vesicles carry cellulases in the industrial fungus Trichoderma reesei.
BIOTECHNOLOGY FOR BIOFUELS,
v. 12,
JUN 15 2019.
Web of Science Citations: 0.
DE PAULA, RENATO GRACIANO;
CAMPOS ANTONIETO, AMANDA CRISTINA;
CARRARO, CLAUDIA BATISTA;
BORGES LOPES, DOUGLAS CHRISTIAN;
PERSINOTI, GABRIELA FELIX;
AGUIAR PERES, NALU TEIXEIRA;
MARTINEZ-ROSSI, NILCE MARIA;
SILVA-ROCHA, RAFAEL;
SILVA, ROBERTO NASCIMENTO.
The Duality of the MAPK Signaling Pathway in the Control of Metabolic Processes and Cellulase Production in Trichoderma reesei.
SCIENTIFIC REPORTS,
v. 8,
OCT 8 2018.
Web of Science Citations: 2.
DE PAULA, RENATO GRACIANO;
CAMPOS ANTONIETO, AMANDA CRISTINA;
COSTA RIBEIRO, LILIANE FRAGA;
CARRARO, CLAUDIA BATISTA;
VIEIRA NOGUEIRA, KAROLINE MARIA;
BORGES LOPES, DOUGLAS CHRISTIAN;
SILVA, ALINNE COSTA;
ZERBINI, MARIANA TAISE;
PEDERSOLI, WELLINGTON RAMOS;
COSTA, MARIANA DO NASCIMENTO;
SILVA, ROBERTO NASCIMENTO.
New Genomic Approaches to Enhance Biomass Degradation by the Industrial Fungus Trichoderma reesei.
INTERNATIONAL JOURNAL OF GENOMICS,
2018.
Web of Science Citations: 6.