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Alternative and innovative methods of induction of haploid, double-haploid and polyploid plants, aiming an application for plant breeding

Abstract

This proposal aims to develop alternative and innovative methodologies for induction of double haploid (DH) and polyploid (triploid and tetraploid) plants for eucalyptus and tobacco. Haploid and DH plants have great interest for breeding programs involving the obtention of inbred lines, followed by crossing for restoration of hybrid vigor (heterosis). Triploid and tetraploid plants have also great interest, generally because they have larger size organs, potential increased tolerance to drought and seedless fruits. Three groups of experiments are being proposed: a) To select eucalyptus plants with distinct ploidy (haploid-n; triploid-3n or tetraploid-4n) in in vitro germinated seedlings obtained from lots of smaller seeds; b) To induce stable tetraploid plants of eucalyptus by in vitro micrografting method with addition of colchicine; c) To induce DH plants of Tobacco by anther culture method with temporary culture in medium containing colchicine, prior to regeneration of plantlets. It is hoped that these alternative methods present greater efficiency, in terms of higher percentage of plants obtained with different ploidy, more polyploid plants with no chimaeras and possible reduction of labor and time to acquire it. There is great expectation regarding the implementation of this project because, at the end of it, we expected that the company will be better structured, with the existence of new tissue culture and molecular markers labs while flow cytometry lab continue to work. Then, the company can expand its scope in a related area to which it operates. It may offer these innovative services to actual and to new customers. It is expected a real growth of the company's structure and sales. (AU)

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