Flavonoids combinations in thermosensitive hidrogels as proposals of endodontic medications for young permanent teeth

• Grant number: 17/10940-1
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2017
• End date: November 30, 2019
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Cristiane Duque
• Grantee: Cristiane Duque
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Associated researchers: Alberto Carlos Botazzo Delbem ; Ana Cláudia Okamoto ; Carlos Alberto de Souza Costa ; Denise Madalena Palomari Spolidorio ; Emerson Rodrigues de Camargo ; Juliano Pelim Pessan ; Sandra Helena Penha de Oliveira

Abstract

The objective of this study was to evaluate the in vitro antimicrobial, antiinflammatory and inductive effects of mineralization of compounds representing the main classes of natural, isolated and combined flavonoids and to study proposals to incorporate these compounds into thermosensitive hydrogels as endodontic medication. The aim of this study was to evaluate the antimicrobial activity of the following flavonoids: taxifolin, pinocembrin, galangin, chrysin, EGCG, quercetin, kaempferol, myricetin, naringin, genistein, daidzein and silibilin, by determining MIC, CBM and CIF on bacteria of endodontic interest. Then, flavonoids with antimicrobial activity, either alone or in combination, will be selected to evaluate the effect on bacterial biofilm by counting the CFU / mL. In macrophage culture, the flavonoids will be screened for cell viability (MTT assay) and subjected to ROS quantification, NO inhibition and qPCR assays for determination of iNOS, COX-2 and IL-1² expression levels. In addition, the compounds will also be evaluated on undifferentiated pulp cells and osteoblasts for the induction of mineralization by determination of ALP activity, quantification of nodules of mineralization and expression of DMP-1, DSPP, OPG, RANKL and OCN. The flavonoids or combinations of compounds with the best results in the above assays will be incorporated into two types of thermosensitive hydrogels: PNVCL or chitosan and evaluated the antibiofilm effect in confocal microscopy and cytotoxicity by means of MTT test and fluorescence microscopy. This study intends to propose an intracanal medication with natural flavonoids with therapeutic properties based on the in vitro results and that may have future clinical application. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DUQUE, CRISTIANE; HUSSEIN, HEBATULLAH; BORTOLATTO, JANAINA; PRAKKI, ANURADHA; KISHEN, ANIL. Effect of taxifolin and epigallocatechin-3-gallate on biomineralization potential of stem cells from dental apical papilla. ARCHIVES OF ORAL BIOLOGY, v. 138, p. 8-pg., 2022-06-01. (17/10940-1)

MASSUNARI, LOIANE; RABELO, RAFAELA LARUZO; LEITE, MARIA LUISA; SOARES, DIANA GABRIELA; ANOVAZZI, GIOVANA; SOUZA COSTA, CARLOS ALBERTO; DUQUE, CRISTIANE. Dose- and time-dependent effects of taxifolin on viability and mineralization markers of osteoblast-like cells. Brazilian Oral Research, v. 35, 2021. (15/00812-0, 17/10940-1, 19/02129-7)

BRAGA, GABRIELA PACHECO DE ALMEIDA; CAIAFFA, KARINA SAMPAIO; PEREIRA, JESSE AUGUSTO; DOS SANTOS, VANESSA RODRIGUES; SOUZA, AMANDA CASELATO ANDOLFATTO; RIBEIRO, LUCAS DA SILVA; CAMARGO, EMERSON RODRIGUES; PRAKKI, ANURADHA; DUQUE, CRISTIANE. Microbiological Properties and Cytotoxicity of PNVCL Hydrogels Containing Flavonoids as Intracanal Medication for Endodontic Therapy. JOURNAL OF FUNCTIONAL BIOMATERIALS, v. 13, n. 4, p. 16-pg., 2022-12-01. (17/10940-1, 18/12871-0)

BRAGA, GABRIELA PACHECO DE ALMEIDA; CAIAFFA, KARINA SAMPAIO; RABELO, RAFAELA LARUZO; DOS SANTOS, VANESSA RODRIGUES; SOUZA, AMANDA CASELATO ANDOLFATTO; RIBEIRO, LUCAS DA SILVA; DE CAMARGO, EMERSON RODRIGUES; PRAKKI, ANURADHA; DUQUE, CRISTIANE. Cytotoxicity and Biomineralization Potential of Flavonoids Incorporated into PNVCL Hydrogels. JOURNAL OF FUNCTIONAL BIOMATERIALS, v. 14, n. 3, p. 17-pg., 2023-03-01. (17/10940-1, 18/12871-0)

CRISTIANE DUQUE; AMANDA CASELATO ANDOLFATTO SOUZA; KELLY LIMI AIDA; JESSE AUGUSTO PEREIRA; KARINA SAMPAIO CAIAFFA; VANESSA RODRIGUES DOS SANTOS; LEOPOLDO COSME-SILVA; ANURADHA PRAKKI. Synergistic antimicrobial potential of EGCG and fosfomycin against biofilms associated with endodontic infections. Journal of Applied Oral Science, v. 31, 2023-03-27. (17/10940-1)