Evaluation of physical exercise effects on cognition of neuronal-PTEN deleted animals

• Grant number: 16/22996-9
• Support Opportunities: Regular Research Grants
• Duration: July 01, 2017 - June 30, 2019
• Field of knowledge: Biological Sciences - Pharmacology - Biochemical and Molecular Pharmacology
• Principal Investigator: Elisa Mitiko Kawamoto Iwashe
• Grantee: Elisa Mitiko Kawamoto Iwashe
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Cristoforo Scavone

Abstract

PTEN (Phosphatase and tensin homolog on chromosome ten) is known as a tumor suppressor with its roles related or not to its phosphatase activity. Studies have been focused to investigate mechanisms relating PTEN to tumorigenesis. However, due to the broad characteristics of this phosphatase which is able to influence cellular proliferation, survival and migration, PTEN seems also to play a potential modulatory role on synaptic plasticity. Changes on physiological function of this signaling pathway are associated with some central nervous system (CNS) pathologies, such as autism spectrum disorder, schizophrenia, etc. Hippocampus and cortex are important brain regions for cognition, playing essential roles in learning and memory processes. Several environmental factors, such as, physical exercise, have been shown to act as synaptic plasticity modulators, contributing to positive cognitive effects.The limitation to study this pathway due to the embryological lethality in the absence of PTEN was overcome with the use of PTEN conditioned knockout mice, which also is a good tool of tissue and time specific deletion, allowing exploring a certain protein physiological role. In this way, this study aims to evaluate, under biochemical and functional parameters, the effects of physical exercise on signaling pathway related to learning and memory, which can be modulated by PTEN deletion in neurons. Therefore, the results of this study can contribute to a better understanding about the potential use of physical exercise as a strategy to prevent or even to treat CNS related pathologies such as neurodegeneration and autism spectrum disorder. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KINOSHITA, PAULA FERNANDA; MARQUES ORELLANA, ANA MARIA; NAKAO, VINICIUS WATANABE; DE SOUZA PORT'S, NATACHA MEDEIROS; MENEZES QUINTAS, LUIS EDUARDO; KAWAMOTO, ELISA MITIKO; SCAVONE, CRISTOFORO. The Janus face of ouabain in Na+/K+-ATPase and calcium signalling in neurons. British Journal of Pharmacology, MAR 2021. (16/22996-9, 19/12974-6, 16/07427-8, 18/14289-6)

CABRAL-COSTA, J. V.; ANDREOTTI, D. Z.; MELLO, N. P.; SCAVONE, C.; CAMANDOLA, S.; KAWAMOTO, E. M.. Intermittent fasting uncovers and rescues cognitive phenotypes in PTEN neuronal haploinsufficient mice. SCIENTIFIC REPORTS, v. 8, JUN 5 2018. (13/20594-2, 15/25491-2, 14/18689-8, 16/22996-9, 11/21308-8)

ANDREOTTI, DIANA ZUKAS; SILVA, JOSIANE DO NASCIMENTO; MATUMOTO, AMANDA MIDORI; ORELLANA, ANA MARIA; DE MELLO, PALOMA SEGURA; KAWAMOTO, ELISA MITIKO. Effects of Physical Exercise on Autophagy and Apoptosis in Aged Brain: Human and Animal Studies. FRONTIERS IN NUTRITION, v. 7, JUL 28 2020. (16/22996-9, 16/07427-8)

KINOSHITA, PAULA FERNANDA; MARQUES ORELLANA, ANA MARIA; NAKAO, VINICIUS WATANABE; DE SOUZA PORT'S, NATACHA MEDEIROS; MENEZES QUINTAS, LUIS EDUARDO; KAWAMOTO, ELISA MITIKO; SCAVONE, CRISTOFORO. The Janus face of ouabain in Na+/K+-ATPase and calcium signalling in neurons. British Journal of Pharmacology, v. 179, n. 8, p. 13-pg., 2021-03-30. (16/22996-9, 18/14289-6, 16/07427-8, 19/12974-6)

DE MOLLO, NATALIA PRUDENTE; ORELLANA, ANA MARIA; MAZUCANTI, CAIO HENRIQUE; LIMA, GEOVANNI DE MORALS; SCAVONE, CRISTOFORO; KAWAMOTO, ELISE MITIKO. Insulin and Autophagy in Neurodegeneration. FRONTIERS IN NEUROSCIENCE, v. 13, MAY 22 2019. (16/22996-9, 16/07427-8)

DE MELLO, NATALIA PRUDENTE; ANDREOTTI, DIANA ZUKAS; ORELLANA, ANA MARIA; SCAVONE, CRISTOFORO; KAWAMOTO, ELISA MITIKO. Inverse sex-based expression profiles of PTEN and Klotho in mice. SCIENTIFIC REPORTS, v. 10, n. 1, NOV 19 2020. (11/21308-8, 16/07427-8, 16/22996-9, 15/25491-2)

KAWAMOTO, E. M.; CARARO-LOPES, M. M.; KINOSHITA, P. F.; QUINTAS, L. E. M.; LIMA, L. S.; ANDREOTTI, D. Z.; SCAVONE, C.. Influence of Nitric Oxide-Cyclic GMP and Oxidative STRESS on Amyloid-beta Peptide Induced Decrease of Na,K-ATPase Activity in Rat Hippocampal Slices. Journal of Membrane Biology, v. 254, n. 5-6, SI, JUL 2021. (16/07427-8, 16/22996-9, 18/14289-6, 19/12974-6, 14/14199-6)

VASCONCELOS, ANDREA R.; DA PAIXAO, AMANDA G.; KINOSHITA, PAULA F.; ORELLANA, ANA M.; SCAVONE, CRISTOFORO; KAWAMOTO, ELISA M.. Toll-like Receptor 4 Signaling is Critical for the Adaptive Cellular Stress Response Effects Induced by Intermittent Fasting in the Mouse Brain. Neuroscience, v. 465, p. 142-153, JUN 15 2021. (14/01435-3, 19/12974-6, 16/22996-9, 16/07376-4, 06/57729-9, 16/07427-8, 11/12255-8, 09/11915-4)