Physiological adjustments of tropical teleosts in anthropogenic reservoirs

• Grant number: 17/11530-1
• Support Opportunities: Regular Research Grants
• Duration: October 01, 2017 - September 30, 2019
• Field of knowledge: Biological Sciences - Physiology - Compared Physiology
• Principal Investigator: Renata Guimarães Moreira Whitton
• Grantee: Renata Guimarães Moreira Whitton
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The increase of the population density promoted the intensification of the anthropic activities in the natural environments, leading to an intense process of degradation and contamination of the water bodies. In the reservoirs of the Metropolitan Region of São Paulo (RMSP), have been reported an intense eutrophication process and the presence of emerging pollutant from the anthropization, like different types of drugs, being diclofenac (DCF) and caffeine (CAF), those which are in the highest concentration. Although the hydrological and ecological processes of the reservoirs are extensively studied, research on the trophic transfer of polyunsaturated fatty acids to fish and the study of the effects of the drugs on the physiology of the animals are very limited. Based on this information, the objective of the present project is to evaluate the physiological adjustments related to the fatty acid metabolism, oxidative stress and endocrinology of reproduction of the species Astyanax fasciatus and A. altiparanae due to the anthropic impact in the reservoirs of the RMSP. For this, four reservoirs of the RMSP (Billings, Guarapiranga, Jundiaí and Ponte Nova) will be sampled during winter and summer. Water samples will be collected for analysis of nutrients and concentration of CAF and DCF; seston and zooplankton samples, as well as tissues from fish will be collected for analysis of fatty acids; oxidative stress enzymes, concentration of prostaglandins, cyclooxygenase and cytosolic phospholipase activity, plasma steroid concentration and plasma vitellogenin (VTG), genic expression of gonadotropins (fsh² and lh²) and VTG, and genotoxicity. The effect of the drugs on oxidative stress and endocrinology of fish reproduction will be tested in acute bioassay. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BRANCO, GIOVANA SOUZA; MOREIRA, RENATA GUIMARAES; BORELLA, MARIA INES; CAMARGO, MARILIA DE PAIVA; MUNOZ-PENUELA, MARCELA; GOMES, ALINE DAL OLIO; TOLUSSI, CARLOS EDUARDO. Nonsteroidal anti-inflammatory drugs act as endocrine disruptors in Astyanax lacustris (Teleostei: Characidae) reproduction: An ex vivo approach. AQUATIC TOXICOLOGY, v. 232, MAR 2021. (17/11530-1, 14/16320-7)

ESCALANTE-ROJAS, MARIA C.; TOLUSSI, CARLOS E.; GOMES, ALINE D.; MUNOZ-PENUELA, MARCELA; BRAMBILA-SOUZA, GABRIELA; BRANCO, GIOVANA S.; MOREIRA, RENATA G.. Integrated use of biomarkers to evaluate the reproductive physiology of Astyanax fasciatus and Hoplias malabaricus males (Teleostei: Characiformes) in polluted reservoirs. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 208, JAN 15 2021. (17/11530-1, 14/16320-7)

MUNOZ-PENUELA, MARCELA; LO NOSTRO, FABIANA LAURA; GOMES, ALINE DAL'OLIO; TOLUSSI, CARLOS EDUARDO; BRANCO, GIOVANA SOUZA; SILVA PINHEIRO, JOAO PAULO; ANDRADE DE GODOI, FILIPE GUILHERME; MOREIRA, RENATA GUIMARAES. Diclofenac and caffeine inhibit hepatic antioxidant enzymes in the freshwater fish Astyanax altiparanae (Teleostei: Characiformes). COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, v. 240, FEB 2021. (17/11530-1, 14/16320-7)

GODOI, FILIPE G. ANDRADE; FORNER-PIQUER, ISABEL; RANDAZZO, BASILIO; HABIBI, HAMID R.; LO NOSTRO, FABIANA L.; MOREIRA, RENATA GUIMARAES; CARNEVALI, OLIANA. Effects of Di-Isononyl Phthalate (DiNP) on Follicular Atresia in Zebrafish Ovary. FRONTIERS IN ENDOCRINOLOGY, v. 12, JUN 14 2021. (17/07139-5, 14/16320-7, 18/10495-0, 17/11530-1)

MARQUES, V. H.; MOREIRA, R. G.; GOMES, A. D.. Influence of tropical reservoirs eutrophication on the polyunsaturated fatty acid profile in Astyanax altiparanae (Pisces: Characidae). Science of The Total Environment, v. 717, MAY 15 2020. (16/07363-0, 17/15662-0, 14/16320-7, 17/11530-1)

GODOI, FILIPE G. A.; MUNOZ-PENUELA, MARCELA; OLIO GOMES, ALINE D.; TOLUSSI, CARLOS E.; BRAMBILA-SOUZA, GABRIELA; BRANCO, GIOVANA S.; LO NOSTRO, FABIANA L.; MOREIRA, RENATA G.. Endocrine disruptive action of diclofenac and caffeine on Astyanax altiparanae males (Teleostei: Characiformes: Characidae). COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, v. 231, MAY 2020. (17/07139-5, 14/16320-7, 17/11530-1)