Advanced search
Start date
Betweenand

Physiological adjustments of tropical teleosts in anthropogenic reservoirs

Grant number: 17/11530-1
Support type:Regular Research Grants
Duration: October 01, 2017 - September 30, 2019
Field of knowledge:Biological Sciences - Physiology
Principal Investigator:Renata Guimarães Moreira Whitton
Grantee:Renata Guimarães Moreira Whitton
Home Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The increase of the population density promoted the intensification of the anthropic activities in the natural environments, leading to an intense process of degradation and contamination of the water bodies. In the reservoirs of the Metropolitan Region of São Paulo (RMSP), have been reported an intense eutrophication process and the presence of emerging pollutant from the anthropization, like different types of drugs, being diclofenac (DCF) and caffeine (CAF), those which are in the highest concentration. Although the hydrological and ecological processes of the reservoirs are extensively studied, research on the trophic transfer of polyunsaturated fatty acids to fish and the study of the effects of the drugs on the physiology of the animals are very limited. Based on this information, the objective of the present project is to evaluate the physiological adjustments related to the fatty acid metabolism, oxidative stress and endocrinology of reproduction of the species Astyanax fasciatus and A. altiparanae due to the anthropic impact in the reservoirs of the RMSP. For this, four reservoirs of the RMSP (Billings, Guarapiranga, Jundiaí and Ponte Nova) will be sampled during winter and summer. Water samples will be collected for analysis of nutrients and concentration of CAF and DCF; seston and zooplankton samples, as well as tissues from fish will be collected for analysis of fatty acids; oxidative stress enzymes, concentration of prostaglandins, cyclooxygenase and cytosolic phospholipase activity, plasma steroid concentration and plasma vitellogenin (VTG), genic expression of gonadotropins (fsh² and lh²) and VTG, and genotoxicity. The effect of the drugs on oxidative stress and endocrinology of fish reproduction will be tested in acute bioassay. (AU)