Multi-user equipment approved in grant 17/02983-2: minispec LF50 body composition mice analyzer

• Grant number: 17/18972-0
• Support Opportunities: Multi-user Equipment Program
• Start date: October 01, 2017
• End date: September 30, 2024
• Field of knowledge: Biological Sciences - Physiology
• Principal Investigator: Jose Donato Junior
• Grantee: Jose Donato Junior
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• City of the host institution: São Paulo
• Associated research grant: 17/02983-2 - The role of growth hormone in the brain: relevance for neural functions and in disease, AP.TEM
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Caracterização de Materiais - Propriedades Mecânicas - Dinâmicas; Caracterização de Materiais - Espectroscopia - Ressonância magnética nuclear
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

The Minispec LF50 (compact magnetic resonance device), manufactured by Bruker, allows the analysis of the body composition of mice up to 60 grams (therefore it is not compatible with rats) using an MRI method, but without generating images. The data provided by the equipment refers to the amount of fat, lean mass and body water in the analyzed animal, in relation to its body weight. Analysis is rapid (30 seconds) and the animal remains semi-immobilized at that time by an acrylic container. A trained user can scan a mouse every 2 minutes. No anesthesia or any other type of sedation is required during this analysis, allowing longitudinal follow-up with minimal stress. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KAKIMOTO, PAMELA A.; CHAUSSE, BRUNO; CALDEIRA DA SILVA, CAMILLE C.; DONATO JUNIOR, JOSE PRIME; KOWALTOWSKI, ALICIA J.. Resilient hepatic mitochondria! function and lack of iNOS dependence in diet-induced insulin resistance. PLoS One, v. 14, n. 2, FEB 4 2019. (13/07937-8, 17/18972-0, 15/25862-0, 15/07670-7)