Proteomic characterization and enzymatic and pathophysiological activities of the venom of the snake species that compose the Bothrops neuwiedi group

• Grant number: 17/16908-2
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2017
• End date: April 30, 2020
• Field of knowledge: Biological Sciences - Biochemistry
• Principal Investigator: Karen de Morais Zani
• Grantee: Karen de Morais Zani
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Envenomation by snakebites is classified by the World Health Organization as a neglected tropical disease, responsible for approximately 2.5 million envenomations and 150,000 deaths per year. In Brazil, species belonging to the genus Bothrops represent the most important group of snakes for public health, accounting for about 86.7% of recorded snakebites. Among these species, the one with the greatest geographic distribution is Bothrops neuwiedi (B. neuwiedi). The snakes that composed the so-called "B. neuwiedi complex" underwent a taxonomic revision in 2008, based on morphological characters. As a result of this analysis, it was proposed that this complex is in fact composed by 7 full species: B. neuwiedi, B. diporus, B. lutzi, B. mattogrossensis, B. pauloensis, B. pubescens and B. marmoratus. Recently, this classification was corroborated on the basis of molecular data, involving mitochondrial and nuclear gene sequences. However, studies involving the proteomic and pathophysiological characterization of the venoms of these species after the taxonomic revision of the group are scarce. Only the venom of B. diporus (from Argentina) and B. pauloensis had their proteome characterized. In contrast, to the best of our knowledge, no studies on the composition and biological activities of the venom is avaliable for B. lutzi, B. marmoratus, B. mattogrossensis and B. pubescens. In addition, although there is a number of studies involving B. neuwiedi venom, new analyzes are necessary considering that most of the existing studies do not specify the subspecies used or use a mixture of venom of different subspecies, according to the former taxonomical classification. Considering the complexity of the B. neuwiedi group and the relevance of these species to public health, a multifaceted study comparing the venom protein composition of these species and their reactivity with the anti-venom produced by the Butantan Institute is of huge importance. The characterization of the venom of the species that compose the B. neuwiedi group will be of great contribution to (i) the knowledge per se of the venom characteristics of each species, including protein composition and pathophysiological activities; (ii) the correlation between the characteristics of the venoms and the phylogenetic relationships of the species under study; and (iii) the investigation of the efficiency of antibothropic serum in neutralizing the venom of each one of these species. (AU)

Scientific publications

(17)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AGUIAR, WESLEI DA SILVA; GALIZIO, NATHALIA DA COSTA; SANT'ANNA, SAVIO STEFANINI; SILVEIRA, GIOVANNI PEREZ M.; RODRIGUES, FABIOLA DE SOUZA; GREGO, KATHLEEN FERNANDES; DE MORAIS-ZANI, KAREN; TANAKA-AZEVEDO, ANITA MITICO. Ontogenetic study of Bothrops jararacussu venom composition reveals distinct profiles. Toxicon, v. 186, p. 67-77, OCT 30 2020. (17/16908-2, 18/25786-0, 18/14724-4, 18/20651-0)

SERINO-SILVA, CAROLINE; MORAIS-ZANI, KAREN; TOYAMA, MARCOS HIKARI; TOYAMA, DANIELA DE OLIVEIRA; GAETA, HENRIQUE HESSE; BITTENCOURT RODRIGUES, CAROLINE FABRI; AGUIAR, WESLEI DA SILVA; TASHIMA, ALEXANDRE KEIJI; GREGO, KATHLEEN FERNANDES; TANAKA-AZEVEDO, ANITA MITICO. Purification and characterization of the first gamma-phospholipase inhibitor (gamma PLI) from Bothrops jararaca snake serum. PLoS One, v. 13, n. 3, MAR 5 2018. (14/11108-0, 13/12826-0, 17/01890-0, 17/16908-2, 12/19321-9, 13/05357-4)

HATAKEYAMA, DANIELA MIKI; TASIMA, LIDIA JORGE; BRAVO-TOBAR, CESAR ADOLFO; SERINO-SILVA, CAROLINE; TASHIMA, ALEXANDRE KEIJI; BITTENCOURT RODRIGUES, CAROLINE FABRI; AGUIAR, WESLEI DA SILVA; GALIZIO, NATHALIA DA COSTA; VENANCIO DE LIMA, EDUARDO OLIVEIRA; KAVAZOI, VICTOR KOITI; et al. Venom complexity of Bothrops atrox (common lancehead) siblings. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 26, OCT 12 2020. (17/16908-2, 18/25786-0, 17/01890-0, 17/20106-9)

LÍDIA J. TASIMA; CAROLINE SERINO-SILVA; DANIELA M. HATAKEYAMA; ERIKA S. NISHIDUKA; ALEXANDRE K. TASHIMA; SÁVIO S. SANTANNA; KATHLEEN F. GREGO; KAREN DE MORAIS-ZANI; ANITA M. TANAKA-AZEVEDO. Crotamine in Crotalus durissus: distribution according to subspecies and geographic origin, in captivity or nature. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 26, 2020-04-06. (14/11108-0, 17/20106-9, 17/16908-2, 17/01890-0, 16/03311-5)

TASIMA, LIDIA J.; HATAKEYAMA, DANIELA M.; SERINO-SILVA, CAROLINE; RODRIGUES, CAROLINE F. B.; DE LIMA, V, EDUARDO O.; SANT'ANNA, SAVIO S.; GREGO, KATHLEEN F.; DE MORAIS-ZANI, KAREN; SANZ, LIBIA; CALVETE, JUAN J.; et al. Comparative proteomic profiling and functional characterization of venom pooled from captive Crotalus durissus terrificus specimens and the Brazilian crotalic reference venom. Toxicon, v. 185, p. 26-35, OCT 15 2020. (17/01890-0, 17/16908-2, 18/25899-0, 17/26533-6, 18/25786-0)

AGUIAR, WESLEI DA SILVA; GALIZIO, NATHALIA DA COSTA; SERINO-SILVA, CAROLINE; SANT'ANNA, SAVIO STEFANINI; GREGO, KATHLEEN FERNANDES; TASHIMA, ALEXANDRE KEIJI; NISHIDUKA, ERIKA SAYURI; DE MORAIS-ZANI, KAREN; TANAKA-AZEVEDO, ANITA MITICO. Comparative compositional and functional analyses of Bothrops moojeni specimens reveal several individual variations. PLoS One, v. 14, n. 9, SEP 12 2019. (17/01890-0, 17/16908-2, 14/11108-0, 17/20106-9, 18/20651-0, 18/14724-4, 18/25786-0)

MACHADO BRAGA, JACQUELINE RAMOS; DE MORAIS-ZANI, KAREN; PEREIRA, DIEGO DOS SANTOS; SANT'ANNA, SAVIO STEFANINI; GALIZIO, NATHALIA DA COSTA; TANAKA-AZEVEDO, ANITA MITICO; GOMES VILARINHO, ARIEL RODRIGUES; RODRIGUES, JOSE LUCCA; TEIXEIRA DA ROCHA, MARISA MARIA. Sexual and ontogenetic variation of Bothrops leucurus venom. Toxicon, v. 184, p. 127-135, SEP 2020. (14/11108-0, 18/14724-4, 17/16908-2, 16/09976-9, 18/25786-0)

GALIZIO, NATHALIA DA COSTA; SERINO-SILVA, CAROLINE; STUGINSKI, DANIEL RODRIGUES; ESTIMA ABREU, PATRICIA ANTONIA; SANT'ANNA, SAVIO STEFANINI; GREGO, KATHLEEN FERNANDES; TASHIMA, ALEXANDRE KEIJI; TANAKA-AZEVEDO, ANITA MITICO; DE MORAIS-ZANI, KAREN. Compositional and functional investigation of individual and pooled venoms from long-term captive and recently wild-caught Bothrops jararaca snakes. JOURNAL OF PROTEOMICS, v. 186, p. 56-70, AUG 30 2018. (14/11108-0, 16/09976-9, 17/01890-0, 17/16908-2)

DE FARIAS, IASMIM BAPTISTA; DE MORAIS-ZANI, KAREN; SERINO-SILVA, CAROLINE; SANT'ANNA, SAVIO S.; DA ROCHA, MARISA M. T.; GREGO, KATHLEEN F.; ANDRADE-SILVA, DEBORA; SERRANO, SOLANGE M. T.; TANAKA-AZEVEDO, ANITA M.. Functional and proteomic comparison of Bothrops jararaca venom from captive specimens and the Brazilian Bothropic Reference Venom. JOURNAL OF PROTEOMICS, v. 174, p. 36-46, MAR 1 2018. (13/07467-1, 16/16935-7, 14/11108-0, 17/01890-0, 17/16908-2, 13/05357-4)

HATAKEYAMA, DANIELA MIKI; TASIMA, LIDIA JORGE; GALIZIO, NATHALIA DA COSTA; SERINO-SILVA, CAROLINE; FABRI BITTENCOURT RODRIGUES, CAROLINE; STUGINSKI, DANIEL RODRIGUES; SANT'ANNA, SAVIO STEFANINI; GREGO, KATHLEEN FERNANDES; TASHIMA, ALEXANDRE KEIJI; NISHIDUKA, ERIKA SAYURI; et al. From birth to adulthood: An analysis of the Brazilian lancehead (Bothrops moojeni) venom at different life stages. PLoS One, v. 16, n. 6, JUN 10 2021. (17/16908-2, 17/20106-9, 18/25786-0, 18/25899-0, 18/14724-4)

BITTENCOURT RODRIGUES, CAROLINE FABRI; SERINO-SILVA, CAROLINE; DE MORAIS-ZANI, KAREN; KAVAZOI, VICTOR KOITI; NOGUEIRA CARVALHO, MARCELO PIRES; GREGO, KATHLEEN FERNANDES; CHIARELLI, TASSIA; TASHIMA, ALEXANDRE KEIJI; TOYAMA, MARCOS HIKARI; TANAKA-AZEVEDO, ANITA MITICO. Boa.PLI: Structural and functional characterization of the gamma phospholipase A2 plasma inhibitor from the non-venomous Brazilian snake Boa constrictor. PLoS One, v. 15, n. 2, FEB 27 2020. (17/20106-9, 17/16908-2, 18/25786-0)

LEANDRO NORBERTO DA SILVA-JÚNIOR; LARA DE SOUZA ABREU; CAROLINE FABRI BITTENCOURT RODRIGUES; NATHÁLIA DA COSTA GALIZIO; WESLEI DA SILVA AGUIAR; CAROLINE SERINO-SILVA; VALDOMIRO SOUZA DOS SANTOS; ISABELLA ALVES COSTA; LUIS VICENTE FRANCO OLIVEIRA; SÁVIO STEFANINI SANTANNA; et al. Geographic variation of individual venom profile of Crotalus durissus snakes. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 26, 2020-08-26. (18/25786-0, 14/11108-0, 16/09976-9, 18/14724-4, 17/16908-2, 17/01890-0)

VENANCIO DE LIMA, EDUARDO OLIVEIRA; TASIMA, LIDIA JORGE; HATAKEYAMA, DANIELA MIKI; SERINO-SILVA, CAROLINE; BITTENCOURT RODRIGUES, CAROLINE FABRI; GALIZIO, NATHALIA DA COSTA; CHIARELLI, TASSIA; NISHIDUKA, ERIKA SAYURI; TEIXEIRA DA ROCHA, MARISA MARIA; SANT'ANNA, SAVIO STEFANINI; et al. Snake venom color and L-amino acid oxidase: An evidence of long-term captive Crotalus durissus terrificus venom plasticity. Toxicon, v. 193, p. 73-83, APR 15 2021. (18/25786-0, 14/11108-0, 17/01890-0, 16/09976-9, 17/20106-9, 17/21052-0, 17/16908-2, 18/14724-4)

SILVA, CAROLINE SERINO; BITTENCOURT RODRIGUES, CAROLINE FABRI; GREGO, KATHLEEN FERNANDES; DE MORAIS-ZAN, KAREN; TANAKA-AZEVEDO, ANITA MITICO. NEUTRALIZATION OF THE LOCAL EFFECTS OF SNAKE VENOM PLA2 BY BOTHROPS JARARACA gamma PHOSPHOLIPASE INHIBITOR (gamma BJPLI). Toxicon, v. 168, p. 1-pg., 2019-10-05. (17/16908-2, 14/11108-0, 17/01890-0, 13/05357-4)

KAVAZOI, VICTOR KOITI; HATAKEYAMA, DANIELA MIKI; SERINO-SILVA, CAROLINE; BITTENCOURT RODRIGUES, CAROLINE FABRI; GREGO, KATHLEEN FERNANDES; MORAIS-ZANI, KAREN; TANAKA-AZEVEDO, ANITA MITICO. IDENTIFICATION AND PURIFICATION OF PHOSPHOLIPASE A2 INHIBITORS (PLIS) FROM SNAKE BLOOD MAINTAINED IN THE LABORATORY OF HERPETOLOGY OF BUTANTAN INSTITUTE. Toxicon, v. 168, p. 1-pg., 2019-10-05. (17/16908-2, 17/17165-3, 17/01890-0)

AGUIAR, WESLEI DA SILVA; GALIZIO, NATHALIA DA COSTA; GREGO, KATHLEEN FERNANDES; SANT'ANNA, SAVIO STEFANINI; DE MORAIS-ZANI, KAREN; TANAKA-AZEVEDO, ANITA MITICO. BOTHROPS JARARACUSSU ONTOGENETIC AND SEASONAL VENOM VARIABILITY: WHAT WE GOT SO FAR. Toxicon, v. 168, p. 1-pg., 2019-10-05. (17/16908-2, 18/20651-0, 17/01890-0)

GALIZIO, NATHALIA DA COSTA; SILVA, CAROLINE SERINO; STUGINSKI, DANIEL RODRIGUES; TEIXEIRA DA ROCHA, MARISA MARIA; DE OLIVEIRA, ROBERTO BAPTISTA; SANT'ANNA, SAVIO STEFANINI; GREGO, KATHLEEN FERNANDES; TANAKA-AZEVEDO, ANITA MITICO; DE MORAIS-ZANI, KAREN. COMPARATIVE STUDY OF PROTEIN PROFILE AND ENZYMATIC ACTIVITIES OF VENOMS FROM SNAKE SPECIES THAT COMPOSE THE BOTHROPS NEUWIEDI COMPLEX. Toxicon, v. 159, p. 1-pg., 2019-03-05. (17/16908-2)