Antigen discovery and development of serological diagnostic methods and vaccine approaches against the Zika Virus (ZIKV)

• Grant number: 16/20045-7
• Support Opportunities: Research Projects - Thematic Grants
• Start date: December 01, 2017
• End date: November 30, 2023
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Luis Carlos de Souza Ferreira
• Grantee: Luis Carlos de Souza Ferreira
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• City of the host institution: São Paulo
• Principal investigators: Edison Luiz Durigon
• Associated research grant(s): 20/06409-1 - Evaluation of humoral immune response and inflammatory response in patients with confirmed diagnosis of COVID-19 at Hospital Sírio Libanês and correlation with disease severity, AP.R; 20/05204-7 - Development of SARS-CoV-2 auto-assembly protein-based nanovaccines, AP.R
• Associated scholarship(s): 19/16975-7 - Clinical and serological follow-up of mothers and their children diagnosed with previous Zika virus infection., BP.IC; 18/24683-3 - Production of envelope protein domain III of Zika Virus in Escherichia coli: evaluation of the protein obtained from soluble fraction and inclusion bodies, BP.DD; 18/20864-3 - Antigen discovery and development of serological diagnostic methods and vaccine approaches against the zika virus (ZIKV), BP.TT; + associated scholarships 18/15280-2 - Antigen Discovery and development of serological diagnostic methods and vaccine approaches against the zika virus (ZIKV), BP.TT; 18/14459-9 - Investigation of zika virus (ZIKV) antigens to develop serological tests and vaccine approaches, BP.PD; 18/08199-4 - Nanovaccines: a novel experimental concept applied to the development of subunit vacines., BP.PD; 18/10913-7 - Development of a new in vitro inhibition strategy for Zika Virus and Influenza Virus using CRISPR-Cas13 RNA-editing technique as an alternative treatment, BP.DD; 18/05346-6 - Development of specific serological tests from recombinant antigens derived from ZIKV, BP.TT; 18/05337-7 - EVALUATION OF SOROLOGICAL DIAGNOSIS TESTE (NS1-REC ELISA) FOR ZIKA VIRUS: ZIKA VIRUS PREVALENCE IN WOMEN IN THE CITY OF SÃO PAULO., BP.IC; 17/24769-2 - Zika virus in postpartum women and newborns: seroepidemiology and molecular characterization, BP.DD - associated scholarships

Abstract

The present grant application for a thematic research project has the main goal to offer financial support to different studies related to the Zika Vírus (ZIKV), presently under development at the Virology (LV) and Vaccine Research Laboratory (LDV) at the University of São Paulo (USP). The work is centered in four main themes: I) generation and application of ZIKV recombinant proteins aiming the development of vaccines and specific serological tests; II) development of experimental in vitro and in vivo models and establishment of biomarkers for the detection and monitoring of the immune responses induced by ZIKV as well as protective immunity induced by vaccine formulations; III) development and testing of different vaccine formulations capable to prevent ZIKV infection, which will include test of different candidate antigens, adjuvants and inoculation routes; and (IV) development of serological tests specific for ZIKV based on different recombinant antigens. The proposed work will be carried out as collaboration with different research groups from USP and other institutions, such as Butantan Institute, Fiocruz and Institute Pasteur. The LV e LDV have established competence in the development of vaccines and serological tests against Dengue Virus (DENV) as well as other virus and pathogens. The proposed projects are supported by the experience of both groups as demonstrated by publications during the last few years. In addition, results already obtained by the groups, in the scope of the present application, have already resulted in publications, patent applications and scientific discoveries which are already under use by different institutions in the country and abroad. The expected results will bring important impacts both on the serology of ZIKV as well as in researches focused on the development of vaccines for human use. The results to be achieved during the project will have relevant academic contributions and will led to the technological developments at short and medium term run. (AU)

Scientific publications

(41)

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

OHKI, CRISTINE MARIE YDE; BENAZZATO, CECILIA; VAN DER LINDEN, VANESSA; FRANCA, JULIA, V; TOLEDO, CARMEN M.; MACHADO, RAFAEL RAHAL GUARAGNA; ARAUJO, DANIELLE BASTOS; OLIVEIRA, DANIELLE BRUNA LEAL; NERIS, ROMULO S.; ASSUNCAO-MIRANDA, IRANAIA; et al. Zika virus infection impairs synaptogenesis, induces neuroinflammation, and could be an environmental risk factor for autism spectrum disorder outcome. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, v. 1870, n. 5, p. 13-pg., 2024-04-17. (13/08844-3, 18/16748-8, 17/24769-2, 11/20683-0, 16/02978-6, 16/20045-7, 17/27131-9)

DE SOUZA, ALEX JUNIOR SOUZA; DE SOUZA, ANTONIO FRANCISCO; ZIMPEL, CRISTINA KRAEMER; AYUPE, MARINA CACADOR; DE ARAUJO, MARCELO VALDEMIR; MACHADO, RAFAEL RAHAL GUARAGNA; SALLES, ERIKA; SALGADO, CAIO LOUREIRO; TAVARES, MARIANA SILVA; SILVA-PEREIRA, TAIANA TAINA; et al. Hepatic endotheliitis in Golden Syrian hamsters (Mesocricetus auratus) experimentally infected with SARS-CoV-2. Revista do Instituto de Medicina Tropical de São Paulo, v. 66, p. 6-pg., 2024-01-01. (21/02736-0, 21/06881-5, 20/06409-1, 23/00532-4, 17/24769-2, 20/07251-2, 19/10896-8, 20/09149-0, 16/20045-7)

VENCESLAU-CARVALHO, ALEXIA ADRIANNE; FAVARO, MARIANNA TEIXEIRA DE PINHO; PEREIRA, LENNON RAMOS; RODRIGUES-JESUS, MONICA JOSIANE; PEREIRA, SAMUEL SANTOS; ANDREATA-SANTOS, ROBERT; ALVES, RUBENS PRINCE DOS SANTOS; CASTRO-AMARANTE, MARIA FERNANDA; RODRIGUES, KARINE BITENCOURT; DA SILVA, JAMILE RAMOS; et al. Nano-multilamellar lipid vesicles loaded with a recombinant form of the chikungunya virus E2 protein improve the induction of virus-neutralizing antibodies. Nanomedicine-Nanotechnology Biology and Medicine, v. 37, OCT 2021. (17/23281-6, 16/19145-7, 16/08204-2, 17/24769-2, 16/20045-7, 16/23560-0)

DANIELLE BASTOS ARAUJO; RAFAEL RAHAL GUARAGNA MACHADO; DEYVID EMANUEL AMGARTEN; FERNANDA DE MELLO MALTA; GABRIEL GUARANY DE ARAUJO; CAIRO OLIVEIRA MONTEIRO; ERIKA DONIZETTI CANDIDO; CAMILA PEREIRA SOARES; FERNANDO GATTI DE MENEZES; ANA CAROLINA CORNACHIONI PIRES; et al. SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network. Memórias do Instituto Oswaldo Cruz, v. 115, 2020-10-23. (16/20045-7, 18/23680-0, 20/06409-1, 20/04680-0, 17/24769-2, 18/21076-9, 17/25123-9)

CASTRO, KARLA R.; SETTI, GRAZIELLE O.; OLIVEIRA, TASSIA R. DE; RODRIGUES-JESUS, MONICA J.; BOTOSSO, VIVIANE F.; ARAUJO, ANA P. PERINI DE; DURIGON, EDSON L.; FERREIRA, LUIS C. S.; FARIA, RONALDO C.. Electrochemical magneto-immunoassay for detection of zika virus antibody in human serum. Talanta, v. 256, p. 8-pg., 2023-02-02. (18/04332-1, 16/20045-7)

RODRIGUES, JULIANA FALCAO; LOURENCO, ROGERIO FERREIRA; MAEDA, DENICAR LINA NASCIMENTO FABRIS; DE JESUS CINTRA, MARIANA; NAKAO, NAOMI; MATHIAS-SANTOS, CAMILA; LUIZ, WILSON BARROS; DE SOUZA FERREIRA, LUIS CARLOS. Strain-specific transcriptional and posttranscriptional regulation of heat-labile toxin expression by enterotoxigenic Escherichia coli. Brazilian Journal of Microbiology, v. 51, n. 2, FEB 2020. (16/20045-7)

ANDREATA-SANTOS, ROBERT; PEREIRA, SAMUEL SANTOS; PEREIRA, LENNON RAMOS; FELIX, ALVINA CLARA; ROMANO, CAMILA MALTA; DE SOUZA FERREIRA, LUIS CARLOS. Specificity of NS1-based immunochromatographic tests for dengue virus with regard to the Zika virus protein. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, v. 95, p. 276-278, JUN 2020. (16/20045-7, 16/23560-0)

PEREIRA, SAMUEL SANTOS; ANDREATA-SANTOS, ROBERT; PEREIRA, LENNON RAMOS; SOARES, CAMILA PEREIRA; FELIX, ALVINA CLARA; JUNGMANN CARDOSO DE ANDRADE, PATRICIA DE MELLO; DURIGON, EDISON LUIS; ROMANO, CAMILA MALTA; DE SOUZA FERREIRA, LUIS CARLOS. NS1-based ELISA test efficiently detects dengue infections without cross-reactivity with Zika virus. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, v. 112, p. 202-204, SEP 2021. (17/09661-0, 16/05570-8, 16/20045-7)

MENDRONE-JUNIOR, ALFREDO; DINARDO, CARLA LUANA; FERREIRA, SUZETE CLEUZA; NISHYA, ANNA; SALLES, NANCI ALVES; DE ALMEIDA NETO, CESAR; HAMASAKI, DEBORA TOSHEI; FACINCANI, TILA; DE OLIVEIRA ALVES, LUCAS BASSOLLI; MACHADO, RAFAEL RAHAL GUARAGNA; et al. Correlation between SARS-COV-2 antibody screening by immunoassay and neutralizing antibody testing. Transfusion, v. 61, n. 4, p. 1181-1190, APR 2021. (16/20045-7, 20/06409-1, 17/24769-2)

MENDES, ERICA ARAUJO; BAIRROS DE PILGER, DENISE REGINA; DE SEIXAS SANTOS NASTRI, ANA CATHARINA; MALTA, FERNANDA DE MELLO; PASCOALINO, BRUNO DOS SANTOS; CARNEIRO D'ALBUQUERQUE, LUIZ AUGUSTO; BALAN, ANDREA; GONDIM DE FREITAS JR, LUCIO HOLANDA; DURIGON, EDISON LUIS; CARRILHO, FLAIR JOSE; et al. Sofosbuvir inhibits yellow fever virus in vitro and in patients with acute liver failure. ANNALS OF HEPATOLOGY, v. 18, n. 6, p. 816-824, NOV-DEC 2019. (16/20045-7)

PINHO FAVARO, MARIANNA TEIXEIRA; RODRIGUES-JESUS, MONICA JOSIANE; VENCESLAU-CARVALHO, ALEXIA ADRIANNE; DOS SANTOS ALVES, RUBENS PRINCE; PEREIRA, LENNON RAMOS; PEREIRA, SAMUEL SANTOS; ANDREATA-SANTOS, ROBERT; DE SOUZA FERREIRA, LUIS CARLOS. Nanovaccine based on self-assembling nonstructural protein 1 boosts antibody responses to Zika virus. Nanomedicine-Nanotechnology Biology and Medicine, v. 32, FEB 2021. (16/20045-7, 18/08199-4)

ARAUJO, SERGIO C.; PEREIRA, LENNON R.; ALVES, RUBENS P. S.; ANDREATA-SANTOS, ROBERT; KANNO, ALEX I.; FERREIRA, LUIS CARLOS S.; GONCALVES, VIVIANE M.. Anti-Flavivirus Vaccines: Review of the Present Situation and Perspectives of Subunit Vaccines Produced in Escherichia coli. VACCINES, v. 8, n. 3, SEP 2020. (18/24683-3, 16/20045-7, 16/23560-0, 19/18205-4, 15/02352-7)

FLOREZ-ALVAREZ, LIZDANY; DE SOUZA, EDMARCIA ELISA; BOTOSSO, VIVIANE FONGARO; LEAL DE OLIVEIRA, DANIELLE BRUNA; HO, PAULO LEE; TABORDA, CARLOS PELLESCHI; PALMISANO, GIUSEPPE; CAPURRO, MARGARETH LARA; REBELLO PINHO, JOAO RENATO; FERREIRA, HELENA LAGE; et al. Hemorrhagic fever viruses: Pathogenesis, therapeutics, and emerging and re-emerging potential. FRONTIERS IN MICROBIOLOGY, v. 13, p. 16-pg., 2022-10-25. (16/20045-7, 19/12303-4, 18/18257-1, 20/04923-0, 17/03966-4, 17/27131-9, 22/01812-8, 18/15549-1, 15/26722-8, 20/12277-0, 20/06409-1, 16/08730-6)

DA SILVA, JAMILE RAMOS; RODRIGUES, KARINE BITENCOURT; PELEGRIN, GUILHERME FORMOSO; SALES, NATIELY SILVA; MURAMATSU, HIROMI; SILVA, MARIANGELA DE OLIVEIRA; PORCHIA, BRUNA F. M. M.; MORENO, ANA CAROLINA RAMOS; APS, LUANA RAPOSO M. M.; VENCESLAU-CARVALHO, ALEXIA ADRIANNE; et al. Single immunizations of self-amplifying or non-replicating mRNA-LNP vaccines control HPV-associated tumors in mice. Science Translational Medicine, v. 15, n. 686, p. 18-pg., 2023-03-08. (16/20045-7, 16/00708-1, 15/16505-0, 16/11594-7, 13/15360-2, 17/21358-1, 19/01523-3, 18/26515-0, 21/03326-0, 18/07629-5, 16/14344-1)

DOS SANTOS, JULIANA DE MELO BATISTA; DO AMARAL, JONATAS BUSSADOR; FRANCA, CAROLINA NUNES; MONTEIRO, FERNANDA RODRIGUES; ALVARES-SARAIVA, ANUSKA MARCELINO; KALIL, SANDRA; DURIGON, EDISON LUIZ; OLIVEIRA, DANIELLE BRUNA LEAL; RODRIGUES, SILVIA SANCHES; HELLER, DEBORA; et al. Distinct Immunological Profiles Help in the Maintenance of Salivary Secretory IgA Production in Mild Symptoms COVID-19 Patients. FRONTIERS IN IMMUNOLOGY, v. 13, p. 13-pg., 2022-05-24. (20/06409-1, 12/15165-2, 19/14115-0, 16/20045-7)

FOTORAN, WESLEY L.; DA SILVA, JAMILE RAMOS; GLITZ, CHRISTIANE; FERREIRA, LUIS CARLOS DE SOUZA; WUNDERLICH, GERHARD. Establishment of an Antiplasmodial Vaccine Based on PfRH5-Encoding RNA Replicons Stabilized by Cationic Liposomes. PHARMACEUTICS, v. 15, n. 4, p. 17-pg., 2023-04-01. (17/24267-7, 21/13727-2, 16/20045-7, 16/19145-7)

NERY, JR., NIVISON; AGUILAR TICONA, JUAN P.; GAMBRAH, CLAUDIA; DOSS-GOLLIN, SIMON; AROMOLARAN, ADEOLU; RASTELY-JUNIOR, VALMIR; LESSA, MILLANI; SACRAMENTO, GIELSON A.; CRUZ, JAQUELINE S.; DE OLIVEIRA, DAIANA; et al. Social determinants associated with Zika virus infection in pregnant women. PLoS Neglected Tropical Diseases, v. 15, n. 7, JUL 2021. (16/20045-7)

SEKINE, LEO; ARNS, BEATRIZ; FABRO, BRUNA R.; CIPOLATT, MURILLO M.; MACHADO, RAFAEL R. G.; DURIGON, EDISON L.; PAROLO, EDINO; PELLEGRINI, JOSE AUGUSTO S.; VIANA, MARINA V.; SCHWARZ, PATRICIA; et al. Convalescent plasma for COVID-19 in hospitalised patients: an open-label, randomised clinical trial. European Respiratory Journal, v. 59, n. 2, p. 11-pg., 2022-02-01. (20/06409-1, 16/20045-7)

PEREIRA, LENNON RAMOS; DOS SANTOS ALVES, RUBENS PRINCE; SALES, NATIELY SILVA; ANDREATA-SANTOS, ROBERT; VENCESLAU-CARVALHO, ALEXIA ADRIANNE; PEREIRA, SAMUEL SANTOS; CASTRO-AMARANTE, MARIA FERNANDA; RODRIGUES-JESUS, MONICA JOSIANE; DE PINHO FAVARO, MARIANNA TEIXEIRA; CHURA-CHAMBI, ROSA MARIA; et al. Enhanced Immune Responses and Protective Immunity to Zika Virus Induced by a DNA Vaccine Encoding a Chimeric NS1 Fused With Type 1 Herpes Virus gD Protein. FRONTIERS IN MEDICAL TECHNOLOGY, v. 2, p. 14-pg., 2020-12-03. (14/17595-0, 16/20045-7, 16/23560-0, 18/14459-9, 16/05570-8, 18/08199-4, 17/09661-0, 16/14344-1)

WENDEL, SILVANO; KUTNER, JOSE MAURO; MACHADO, RAFAEL; FONTAO-WENDEL, RITA; BUB, CAROLINA; FACHINI, ROBERTA; YOKOYAMA, ANA; CANDELARIA, GABRIELA; SAKASHITA, ARACI; ACHKAR, RUTH; et al. Screening forSARS-CoV-2 antibodies in convalescent plasma in Brazil: Preliminary lessons from a voluntary convalescent donor program. Transfusion, v. 60, n. 12, p. 2938-2951, DEC 2020. (18/23680-0, 17/24769-2, 16/20045-7, 20/06409-1)

AGUILAR TICONA, JUAN P.; NERY, NIVISON; DOSS-GOLLIN, SIMON; GAMBRAH, CLAUDIA; LESSA, MILLANI; RASTELY-JUNIOR, VALMIR; MATOS, ADRIANA; FREITAS, BRUNO DE PAULA; BORJA, ANA; WUNDER, ELSIO A.; et al. Heterogeneous development of children with Congenital Zika Syndrome-associated microcephaly. PLoS One, v. 16, n. 9, SEP 15 2021. (16/20045-7)

MACHADO, RAFAEL R. G.; GLASER, TALITA; ARAUJO, DANIELLE B.; PETIZ, LYVIA LINTZMAIER; OLIVEIRA, DANIELLE B. L.; DURIGON, GIULIANA S.; LEAL, ALESSANDRA L.; PINHO, JOAO RENATO R.; FERREIRA, LUIS C. S.; ULRICH, HENNING; et al. Inhibition of Severe Acute Respiratory Syndrome Coronavirus 2 Replication by Hypertonic Saline Solution in Lung and Kidney Epithelial Cells. ACS PHARMACOLOGY & TRANSLATIONAL SCIENCE, v. 4, n. 5, p. 1514-1527, OCT 8 2021. (20/06409-1, 19/00195-2, 18/07366-4, 17/24769-2, 16/20045-7, 20/04680-0)

GOMES, VINICIUS DE MORAIS; SANTOS, DEIVID MARTINS; MACEDO-DA-SILVA, JANAINA; LAZARI, LUCAS C.; MACHADO, RAFAEL RAHAL GUARAGNA; DOS SANTOS, ANCELY FERREIRA; ARAUJO, DANIELLE BASTOS; COUTINHO, JOAO VITOR PACCINI; ARINI, GABRIEL SANTOS; ANGELI, CLAUDIA B.; et al. P.1 and P.2 SARS-CoV-2 Brazilian variants activate the unfolded protein response with a time and pathway specificity. JOURNAL OF PROTEOMICS, v. 315, p. 17-pg., 2025-02-21. (20/06747-4, 16/04676-7, 15/26722-8, 19/09517-2, 22/13150-0, 21/14179-9, 20/06409-1, 18/15549-1, 18/07142-9, 18/18257-1, 17/24769-2, 16/20045-7, 20/04923-0, 21/00140-3)

MACHADO, RAFAEL RAHAL GUARAGNA; PERES, MARCELLA SANCHES; PEREIRA, SAMUEL SANTOS; MELLO, RALYRIA; ARAUJO, DANIELLE BASTOS; PEREIRA, CAMILA SOARES; PASTORINO, BORIS; OLIVEIRA, DANIELLE BRUNA LEAL; FALEIROS, MICHELE DA SILVA JORDAN; FRANSCISCO, ROSSANA PULCINELI VIEIRA; et al. Seroprevalence and Placental Transfer of Zika and Dengue Virus Antibodies in Postpartum Women in Southeast Brazil. Journal of Medical Virology, v. 97, n. 5, p. 15-pg., 2025-05-01. (16/20045-7, 19/16975-7, 20/06409-1, 17/24769-2)

STAN, RAZVAN C.; FRANCOSO, KATIA S.; ALVES, RUBENS P. S.; FERREIRA, LUIS CARLOS S.; SOARES, IRENE S.; DE CAMARGO, MARISTELA M.. Febrile temperatures increase in vitro antibody affinity for malarial and dengue antigens. PLoS Neglected Tropical Diseases, v. 13, n. 4, APR 2019. (11/51778-6, 12/13032-5, 14/17595-0, 16/20045-7)

KANNO, ALEX ISSAMU; DE CERQUEIRA LEITE, LUCIANA CEZAR; PEREIRA, LENNON RAMOS; RODRIGUES DE JESUS, MONICA JOSIANE; ANDREATA-SANTOS, ROBERT; DOS SANTOS ALVES, RUBENS PRINCE; DURIGON, EDISON LUIZ; DE SOUZA FERREIRA, LUIS CARLOS; GONCALVES, VIVIANE MAIMONI. Optimization and scale-up production of Zika virus Delta NS1 in Escherichia coli: application of Response Surface Methodology. AMB EXPRESS, v. 10, n. 1, DEC 2020. (12/23124-4, 14/17595-0, 16/20045-7)

RODRIGUES, JULIANA FALCAO; LOURENCO, ROGERIO FERREIRA; MAEDA, DENICAR LINA NASCIMENTO FABRIS; DE JESUS CINTRA, MARIANA; NAKAO, NAOMI; MATHIAS-SANTOS, CAMILA; LUIZ, WILSON BARROS; DE SOUZA FERREIRA, LUIS CARLOS. Strain-specific transcriptional and posttranscriptional regulation of heat-labile toxin expression by enterotoxigenic Escherichia coli. Brazilian Journal of Microbiology, v. 51, n. 2, p. 11-pg., 2020-02-03. (16/20045-7)

OLIVEIRA, DANIELLE B. L.; DURIGON, GIULIANA S.; MENDES, ERICA A.; LADNER, JASON T.; ANDREATA-SANTOS, ROBERT; ARAUJO, DANIELLE B.; BOTOSSO, VIVIANE F.; PAOLA, NICHOLAS D.; NETO, DANIEL F. L.; CUNHA, MARIELTON P.; et al. Persistence and Intra-Host Genetic Evolution of Zika Virus Infection in Symptomatic Adults: A Special View in the Male Reproductive System. Viruses-Basel, v. 10, n. 11, NOV 2018. (13/07467-1, 14/17766-9, 14/27228-4, 14/16333-1, 16/08727-5, 15/25643-7, 14/03911-7, 16/08204-2, 16/20045-7, 13/26942-2, 16/03605-9)

ANDREATA-SANTOS, ROBERT; DOS SANTOS ALVES, RUBENS PRINCE; PEREIRA, SARA ARAUJO; PEREIRA, LENNON RAMOS; DE FREITAS, CARLA LONGO; PEREIRA, SAMUEL SANTOS; VENCESLAU-CARVALHO, ALEXIA ADRIANNE; CASTRO-AMARANTE, MARIA FERNANDA; PINHO FAVARO, MARIANNA TEIXEIRA; MATHIAS-SANTOS, CAMILA; et al. Transcutaneous Administration of Dengue Vaccines. Viruses-Basel, v. 12, n. 5, MAY 2020. (16/05570-8, 12/50362-3, 16/20045-7, 14/17595-0, 16/23560-0, 15/02352-7)

SLAVOV, SVETOSLAV NANEV; GUARAGNA MACHADO, RAFAEL RAHAL; FERREIRA, ALEXANDER RODRIGO; SOARES, CAMILA PEREIRA; ARAUJO, DANIELLE BASTOS; LEAL OLIVEIRA, DANIELLE BRUNA; COVAS, DIMAS TADEU; DURIGON, EDISON LUIZ; KASHIMA, SIMONE. Zika virus seroprevalence in blood donors from the Northeastern region of Sao Paulo State, Brazil, between 2015 and 2017. Journal of Infection, v. 80, n. 1, p. 111-115, JAN 2020. (17/23205-8, 16/20045-7, 16/15049-3, 16/08727-5, 14/50947-7)

PRUDENCIO, CARLOS ROBERTO; DA COSTA, VIVALDO GOMES; ROCHA, LETICIA BARBOZA; DA COSTA, HERNAN HERMES MONTEIRO; ORTS, DIEGO JOSE BELATO; SANTOS, FELIPE ROCHA DA SILVA; RAHAL, PAULA; LINO, NIKOLAS ALEXANDER BORSATO; DA CONCEICAO, PAMELA JOYCE PREVIDELLI; BITTAR, CINTIA; et al. Identification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological Tests. Viruses-Basel, v. 15, n. 3, p. 20-pg., 2023-03-01. (16/20045-7, 16/16188-7, 17/50333-7, 16/23560-0, 15/17178-2, 16/05570-8, 17/24769-2)

DE CASTRO-AMARANTE, MARIA FERNANDA; PEREIRA, SAMUEL SANTOS; PEREIRA, LENNON RAMOS; SANTOS, LUCAS SOUZA; VENCESLAU-CARVALHO, ALEXIA ADRIANNE; MARTINS, EDUARDO GIMENES; BALAN, ANDREA; FERREIRA, LUIS CARLOS DE SOUZA. The Anti-Dengue Virus Peptide DV2 Inhibits Zika Virus Both In Vitro and In Vivo. Viruses-Basel, v. 15, n. 4, p. 11-pg., 2023-04-01. (18/20162-9, 16/20045-7, 22/01034-5, 18/14459-9, 16/05570-8)

ANDREATA-SANTOS, ROBERT; MACHADO, RAFAEL RAHAL GUARAGNA; DOS SANTOS ALVES, RUBENS PRINCE; SALES, NATIELY SILVA; SOARES, CAMILA PEREIRA; RODRIGUES, KARINE BITENCOURT; SILVA, MARIANGELA OLIVEIRA; FAVARO, MARIANNA TEIXEIRA DE PINHO; RODRIGUES-JESUS, MONICA JOSIANE; YAMAMOTO, MARCIO MASSAO; et al. Validation of Serological Methods for COVID-19 and Retrospective Screening of Health Employees and Visitors to the Sao Paulo University Hospital, Brazil. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 12, p. 8-pg., 2022-06-02. (20/08943-5, 16/20045-7, 21/05661-1, 14/50890-5, 16/23560-0, 15/02352-7, 20/06409-1, 17/24769-2, 16/14344-1, 18/23680-0, 20/10700-3, 18/07629-5, 18/07142-9)

WENDEL, SILVANO; FONTAO-WENDEL, RITA; FACHINI, ROBERTA; CANDELARIA, GABRIELA; SCURACCHIO, PATRICIA; ACHKAR, RUTH; BRITO, MAYRA; REIS, LUIZ FERNANDO; CAMARGO, ANAMARIA; AMANO, MARIANE; et al. A longitudinal study of convalescent plasma (CCP) donors and correlation of ABO group, initial neutralizing antibodies (nAb), and body mass index (BMI) with nAb and anti-nucleocapsid (NP) SARS-CoV-2 antibody kinetics: Proposals for better quality of CCP collections. Transfusion, v. 61, n. 5, FEB 2021. (18/23680-0, 17/24769-2, 16/20045-7, 20/06409-1)

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