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Development and evaluation of biogenic metal nanoparticles (silver, titanium and iron) based on Trichoderma harzianum for agricultural application


Nanotechnology applied to the agricultural area is a reality, studies show that this technology can aid in fertilization and agricultural pests containment resulting in better crop performance. However, there is still a consensus that there is a need for further studies to ensure its effectiveness and safety. Among the possibilities existing the green synthesis of metallic nanoparticles emerge as a promise for an agriculture due to the sustainability appeal. The aim of the proposed project is the synthesis of metallic biogenic nanoparticles using Trichoderma harzianum (a mycoparasite used in biological control) as a reducing agent, for application in control of Sclerotinia sclerotiorum (white mold). The synthesis of metal biogenic nanoparticles results in a synergy between metal and reducing agent, which provides in addition to differentiated physicochemical and positive features, besides providing a coating that presents differentiated characteristics regarding the manipulated metabolite, which will to allow greater potentiality of the properties of the nanoparticle in question. In this work, initially T. harzianum will be stimulated with S. sclerotiorum to obtain metabolites of interest, which are used later for the synthesis of the nanoparticles. To evaluate the activity of the nanoparticles, in vitro analyses will be performed, as well as microcosm evaluation. The cito and genotoxicity of the nanoparticles will be monitored using assays with cell lines, soil and plant microbiota using indirect viability analyses with mitochondrial and lysosomal metabolism evaluation, as well as direct analysis using image cytometry and real-time viability for the evaluation of genotoxicity Analysis of Allium cepa, comet and molecular genetics will be carried out. As results are expected biogenic nanoparticles that have specific coating, which will enable greater efficiency t control of S. sclerotiorum proliferation, among other pathogens. It is also expected that these nanoparticles due to their biogenic characteristic have limited toxicity ensuring the application success in the agricultural area. (AU)

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GUILGER-CASAGRANDE, MARIANA; DE BARROS, CECILIA T.; ANTUNES, VITORIA A. N.; DE ARAUJO, DANIELE R.; LIMA, RENATA. Perspectives and Challenges in the Fight Against COVID-19: The Role of Genetic Variability. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 11, MAR 15 2021. Web of Science Citations: 0.
GUILGER-CASAGRANDE, MARIANA; GERMANO-COSTA, TAIS; BILESKY-JOSE, NATALIA; PASQUOTO-STIGLIANI, TATIANE; CARVALHO, LUCAS; FRACETO, LEONARDO F.; DE LIMA, RENATA. Influence of the capping of biogenic silver nanoparticles on their toxicity and mechanism of action towards Sclerotinia scierotiorum. JOURNAL OF NANOBIOTECHNOLOGY, v. 19, n. 1 FEB 24 2021. Web of Science Citations: 0.
CASAGRANDE, MARIANA GUILGER; DE LIMA, RENATA. Synthesis of Silver Nanoparticles Mediated by Fungi: A Review. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v. 7, OCT 22 2019. Web of Science Citations: 0.
GUILGER-CASAGRANDE, MARIANA; GERMANO-COSTA, TAIS; PASQUOTO-STIGLIANI, TATIANE; FRACETO, LEONARDO FERNANDES; DE LIMA, RENATA. Biosynthesis of silver nanoparticles employing Trichoderma harzianum with enzymatic stimulation for the control of Sclerotinia sclerotiorum. SCIENTIFIC REPORTS, v. 9, OCT 4 2019. Web of Science Citations: 0.
LIMA, RENATA; DEL FIOL, FERNANDO SA; BALCAO, VICTOR M. Prospects for the Use of New Technologies to Combat Multidrug-Resistant Bacteria. FRONTIERS IN PHARMACOLOGY, v. 10, JUN 21 2019. Web of Science Citations: 2.

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