Virus inhibitor substances from Nyctaginaceae and Phytolaccaceae native species as plant defense inducers

• Grant number: 16/25708-4
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2017
• End date: April 30, 2020
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Lígia Maria Lembo Duarte
• Grantee: Lígia Maria Lembo Duarte
• Host Institution: Instituto Biológico (IB). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil
• Associated researchers: Alexandre Levi Rodrigues Chaves ; Déborah Yara Alves Cursino dos Santos ; Luís Carlos Bernacci ; Marcelo Eiras ; Maria Amelia Vaz Alexandre ; Ricardo Harakava

Abstract

Plant viruses can cause heavy damages and losses in major crops worldwide. Their control is not always efficient. Alternative control measures have been tested, and species containing substances inhibiting viral infection highlighting those from the Caryophyllales order, as Nyctaginaceae and Phytolaccaceae have shown promising results. Brazil has one of the richest flora in the world, and native species have an unexploited potential as antiviral principle sources that could be used. Accordingly, it is intended in this project to evaluate the inhibitory activity of viral infection of native species Guapira opposita (Vell.) Reitz and Pisonia ambigua Heimerl (Nyctaginaceae) and Seguieria langsdorffii Moq. and Gallesia integrifolia (Spreng.) Harms (Phytolaccaceae) as well as to evaluate the effect of plant extracts as plant defense inducers. The leaf extracts from species that present viral infection inhibition superior to 50% in Tobacco mosaic virus (TMV) x Nicotiana glutinosa L. system will be selected for tests in Cucurbita pepo x Zuchinni yellow mosaic virus (ZYMV), which presents systemic infection. The extracts will be evaluated at different dilutions as well as at time intervals between treatment and ZYMV inoculation. Other aspects that will be evaluated refer to the parameters related to defense signaling in C. pepo, such as hydrogen peroxide, nitric oxide, salicylic and jasmonic acids and polyamines in four treatments: (i) plants treated with leaf extract(s) from Nyctaginaceae and Phytolaccaceae selected species, (ii) plants treated with phosphate buffer only, (iii) plants inoculated with ZYMV and (iv) treated and inoculated plants. In addition, the metabolomic profile as well as expression of the gene coding for proteins related to the pathogenesis (PR) of C. pepowill also be evaluated in the four treatments by means of gas chromatography coupled to mass spectrometry and qRT-PCR, respectively. The realization of this project will not only open new perspectives of alternative control of economically important plant viruses through the use of native species of the Brazilian flora but will also provide information for studies related to the mechanism of action, as well as the interactions in the defense processes as reactive oxygen species elicitors, PR gene expression and cascade of defense-related reactions. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEREIRA, MARCOS VINICIUS M.; ALEXANDRE, V, MARIA AMELIA; CHAVES, ALEXANDRE LEVI R.; DE SOUZA, ANA CLAUDIA O.; BERNACCI, LUIS CARLOS; NAGAI, ALICE; DOS SANTOS, DEBORAH YARA A. C.; DUARTE, LIGIA MARIA L.. Espécies de Caryophyllales endêmicas da Mata Atlântica como inibidoras de infecção viral em abobrinha-de-moita. Horticultura Brasileira, v. 39, n. 2, p. 146-154, APR-JUN 2021. (16/25708-4)

NAGAI, ALICE; DOS SANTOS, DEBORAH YARA ALVES CURSINO; ALEXANDRE, MARIA AMELIA VAZ; CHAVES, ALEXANDRE LEVI RODRIGUES; FERREIRA, DEMETRIUS RAFAEL TONIN; DUARTE, LIGIA MARIA LEMBO. Putative priming effect of Seguieria langsdorffii Moq. leaf extract in Cucurbita pepo L. infected by zucchini yellow mosaic virus. Rev. bras. Bot., v. N/A, p. 11-pg., 2022-12-29. (16/25708-4)

LEMBO DUARTE, LIGIA MARIA; VAZ ALEXANDRE, MARIA AMELIA; RODRIGUES CHAVES, ALEXANDRE LEVI; ALVES CURSINO DOS SANTOS, DEBORAH YARA; OLIVEIRA DE SOUZA, ANA CLAUDIA; BERNACCI, LUIS CARLOS. Plant-virus infection inhibitors: The great potential of Caryophyllales species. PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, v. 113, JAN 2021. (16/25708-4)