Immunostimulation of Nile tilapia, Oreochromis niloticus fed with probiotic and vaccinated against Streptococcus agalactiae

• Grant number: 17/03738-1
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2018
• End date: October 31, 2020
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Leonardo Tachibana
• Grantee: Leonardo Tachibana
• Host Institution: Instituto de Pesca. Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil
• Associated researchers: Carlos Massatoshi Ishikawa ; Danielle de Carla Dias ; Elaine Fender de Andrade Talmelli ; Erna Elisabeth Bach ; Fábio Rosa Sussel ; Giovani Sampaio Gonçalves ; Maria José Tavares Ranzani de Paiva
• Associated scholarship(s): 18/11337-0 - Immunostimulation of Nile Tilapia, Oreochromis niloticus fed with probiotic and vaccinated against Streptococcus agalactiae, BP.TT; 18/05610-5 - Immunostimulation of Nile tilapia, Oreochromis niloticus fed with probiotic and vaccinated against Streptococcus agalactiae, BP.TT

Abstract

The intensive production systems generally increases the infeccious deseases outbreaks that cause damages for aquaculture. The Streptococcus agalactiae is been considered an example of pathogenic bacteria with major occurrence and economic importance in fish farming. Therefore, this project aimed to evaluate the imulogical parameters and survivel of Nile tilapia fed with diet that contains probiotic and vaccined against S. agalactiae; submited to challenges related to experimental infection. The experiment will be conducted at APTA/SAA - Pólo Centro Leste - Pirassununga UPD. The experimental design will be in completely randomized, in factorial scheme: 2x2+2 controls group, corresponding to six treatments (with or without probiotic inclusion in diet, with or without vaccination, with or without vaccine booster injection). The experiment will be divided in three phases: 1°) in vitro bacteria inibition test; 2°) Nile tilapia anti IgM development; 3°) infection chalenge with S. agalactiae. For imunological response evaluation, following analysis will be done: lisozyme assays, immunoglobuline detection by serum agglutination and ELISA methods. The results will be submited to Analysis of Variance (ANOVA) and Tukey's test (p<0.05). Keywords: infection, imunization, booster, Streptococcus agalactiae. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GUIMARAES, MATEUS CARDOSO; CEREZO, ISABEL M.; FERNANDEZ-ALARCON, MIGUEL FREDERICO; NATORI, MARIENE MIYOKO; SATO, LUCIANA YURI; KATO, CAMILA A. T.; MORINIGO, MIGUEL ANGEL; TAPIA-PANIAGUA, SILVANA; DIAS, DANIELLE DE CARLA; ISHIKAWA, CARLOS MASSATOSHI; et al. Oral Administration of Probiotics (Bacillus subtilis and Lactobacillus plantarum) in Nile Tilapia (Oreochromis niloticus) Vaccinated and Challenged with Streptococcus agalactiae. FISHES, v. 7, n. 4, p. 18-pg., 2022-08-01. (19/20441-8, 17/23225-9, 17/03738-1)