| Grant number: | 17/06460-4 |
| Support Opportunities: | Regular Research Grants |
| Duration: | February 01, 2018 - July 31, 2020 |
| Field of knowledge: | Biological Sciences - Parasitology - Entomology and Malacology of Parasites and Vectors |
| Principal Investigator: | João Aristeu da Rosa |
| Grantee: | João Aristeu da Rosa |
| Host Institution: | Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil |
| Associated researchers: | Daniel Lassere Frías ; Mara Cristina Pinto ; Reinaldo Otávio Alvarenga Alves de Brito |
Abstract
Chagas' disease has as the etiological agent the protozoan Trypanosoma cruzi and the main form of transmission to humans is the vector through triatomines. The subfamily Triatominae has 18 genera and 152 species, of which two are fossils. The control of triatomines is necessary since it is the most efficient way to prevent the transmission of Chagas' disease. Thus, in order to disseminate the specific recognition of triatomines, we intend to obtain, computerize and make available images of these vectors. In order to do so, we will use images of 55 species of triatomines kept alive in 194 colonies in the "Insetário de Triatominae da Faculdade de Ciências Farmacêuticas / UNESP / Araraquara" and others that are obtained. It is also intended to provide images of the genus Mepraia that includes the species: M. gajardoi, M. parapatrica and M. spinolai. These three species occur only in Chile. The females of the three species are all micropterous, but the males are not. The males of M. gajardoi are brachypteros; Those of M. parapatrica macropterous or brachypterous and those of M. spinolai can be macropterous, brachypterous or micropterous. As in the subfamily Triatominae only this genus with sexual dimorphism is known in the alares, it is proposed the study of its three species. Thus, we intend to investigate genetic differences in species of this genus, for which will be made libraries of total RNA of cephalic tissues from next-generation sequencing strategies (RNA-seq). It is also intended to find possible differential profiles of gene expression in transcripts of salivary head and gland of females and males. Comparisons of the genetic profiles of M. gajardoi, M. parapatrica and M. spinolai obtained by RNA-seq will help to understand the evolutionary relationship in species of this genus and also of the subfamily Triatominae. For the development of this project will require collections to be made in Chile, so we will have the support of Professor Daniel Frias Lasserre of the Institute of Entomology of the Metropolitan University of Education Sciences, Santiago, Chile. For the study of transcriptomas we will have the support of Professor Dr. Reinaldo Brito of the Department of Genetics and Evolution of the Federal University of São Carlos. The image bank will be useful to help identify the species by the lay public, scientific and professionals involved in vector control campaigns linked to municipal health secretariats. (AU)
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