G-protein coupled receptors and autophagy: potential targets of omega-3 and deflazacort in DMD therapy

• Grant number: 17/24051-4
• Support type: Regular Research Grants
• Duration: February 01, 2018 - January 31, 2020
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Maria Julia Marques
• Grantee: Maria Julia Marques
• Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Assoc. researchers: Elaine Minatel ; Humberto Santo Neto ; Samara Camaçarí de Carvalho

Abstract

Duchenne muscular dystrophy (DMD) is a progressive degenerative disease that affects skeletal and cardiac muscles, leading to death due to cardiorespiratory failure. Although there is no cure for this disease, recent gene and drug therapies are being able to slow down the progression of the disease, improving life quality and duration. So far, corticoids, such as deflazacort, are still the choice therapy for DMD but, due to their side-effects, there is an urgent need for alternative therapies. We previously demonstrated positive effects of omega-3 in the mdx mice model of DMD. Omega-3 is already in use to treat several inflammatory diseases, in humans. Considering that corticoid therapy is still the best choice, we combined omega-3 and deflazacort and the promising results motivated us to go further on omega-3 mechanisms and effects. In the present study, we will evaluate the G protein-coupled receptors GPR40 and GPR120, which are free fatty acid receptors suggested to mediate omega-3 effects in other systems, and the roles of autophagy, as potential targets of omega-3 and deflazacort, in the diaphragm, quadriceps and cardiac muscles of the dystrophin-deficient mdx mouse. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MACIEL JUNIOR, MARCOS; CAMACARI DE CARVALHO, SAMARA; SAENZ SUAREZ, PAULA ANDREA; SANTO NETO, HUMBERTO; MARQUES, MARIA JULIA. Fish oil attenuated dystrophic muscle markers of inflammation via FFA1 and FFA4 in the mdx mouse model of DMD. Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology, NOV 2020. Web of Science Citations: 0.

FINKLER, JULIA M. G.; DE CARVALHO, SAMARA C.; NETO, HUMBERTO SANTO; MARQUES, MARIA J. Cardiac and skeletal muscle changes associated with rosuvastatin therapy in dystrophic mdx mice. Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology, v. 303, n. 8 DEC 2019. Web of Science Citations: 0.