Synthesis and antibiofilm effect of two new carrier nanosystems of antifungal drugs

• Grant number: 17/24416-2
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2018
• End date: February 29, 2020
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Douglas Roberto Monteiro
• Grantee: Douglas Roberto Monteiro
• Host Institution: Faculdade de Ciências da Saúde (FCSA). Universidade do Oeste Paulista (UNOESTE). Presidente Prudente , SP, Brazil
• Associated researchers: Emerson Rodrigues de Camargo

Abstract

The aim of this study is to synthesize two new carrier nanosystems of antifungal drugs (DRU) (miconazole and fluconazole) based on magnetic iron oxide nanoparticles (MNPs) and chitosan (CTS), as well as to evaluate the antimicrobial effect of these nanosystems against Candida albicans and Candida glabrata. The nanosystems will be prepared by loading of DRU on MNPs coated with CTS and characterized by X-ray diffraction, infrared spectroscopy, thermogravimetric analysis and transmission electron microscopy. The minimum inhibitory concentration of the DRU-CTS-MNP nanosystems capable of inhibiting the strains in planktonic state will be determined according to the microdilution method. After, biofilms of the abovementioned species will be formed (for 24 hours) in 96-well plates and treated twice a day, during 2 days, with different concentrations of the nanosystems. Antibiofilm effect of the nanosystems will be evaluated by counting the number of cultivable cells, total biomass (crystal violet staining assay), metabolic activity (XTT reduction assay), composition of the extracellular matrix (proteins and carbohydrates) and analysis of biofilms' structure. Appropriate controls will be included in all analyzes. Data will be analyzed to employ the most appropriate statistical test. P < .05 will be considered statistically significant. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MORAIS CALDEIRAO, ANNE CAROLINE; ARAUJO, HEITOR CEOLIN; ARIAS, LAIS SALOMAO; CARMONA, WILMER RAMIREZ; MIRANDA, GUSTAVO PORANGABA; PENHA OLIVEIRA, SANDRA HELENA; PESSAN, JULIANO PELIM; MONTEIRO, DOUGLAS ROBERTO. Nanocarriers of Miconazole or Fluconazole: Effects on Three-Species Candida Biofilms and Cytotoxic Effects In Vitro. JOURNAL OF FUNGI, v. 7, n. 7, JUL 2021. (17/24416-2)

ARAUJO, HEITOR CEOLIN; ARIAS, LAIS SALOMAO; MORAIS CALDEIRAO, ANNE CAROLINE; DE FREITAS ASSUMPCAO, LANAY CAROLINE; MORCELI, MARCELA GRIGOLETTO; DE SOUZA NETO, FRANCISCO NUNES; DE CAMARGO, EMERSON RODRIGUES; PENHA OLIVEIRA, SANDRA HELENA; PESSAN, JULIANO PELIM; MONTEIRO, DOUGLAS ROBERTO. Novel Colloidal Nanocarrier of Cetylpyridinium Chloride: Antifungal Activities on Candida Species and Cytotoxic Potential on Murine Fibroblasts. JOURNAL OF FUNGI, v. 6, n. 4, DEC 2020. (17/24416-2)

DE LIMA, TAYNARA M. T.; ARIAS, LAIS S.; AFANACI, LETICIA F.; FERRARESSE, RAPHAEL F. B.; NETO, FRANCISCO N.; DE LIMA, BRUNO H. R.; STRAIOTO, FABIANA G.; DE CAMARGO, EMERSON R.; PESSAN, JULIANO P.; MONTEIRO, DOUGLAS R.. Assembly and antifungal effect of a new fluconazole-carrier nanosystem. FUTURE MICROBIOLOGY, v. 15, n. 4, p. 273-286, MAR 2020. (17/24416-2, 17/08976-8, 17/08980-5)

ARAUJO, HEITOR CEOLIN; GOMES DA SILVA, ANA CAROLINA; PAIAO, LUANA ISABEL; WATANABE MAGARIO, MYCHELLE KEIKO; TFAILE FRASNELLI, SABRINA CRUZ; PENHA OLIVEIRA, SANDRA HELENA; PESSAN, JULIANO PELIM; MONTEIRO, DOUGLAS ROBERTO. Antimicrobial, antibiofilm and cytotoxic effects of a colloidal nanocarrier composed by chitosan-coated iron oxide nanoparticles loaded with chlorhexidine. Journal of Dentistry, v. 101, p. 8-pg., 2020-10-01. (17/24416-2)

ARIAS, LAIS SALOMAO; PESSAN, JULIANO PELIM; DE SOUZA NETO, FRANCISCO NUNES; RAMOS LIMA, BRUNO HENRIQUE; DE CAMARGO, EMERSON RODRIGUES; RAMAGE, GORDON; BOTAZZO DELBEM, ALBERTO CARLOS; MONTEIRO, DOUGLAS ROBERTO. Novel nanocarrier of miconazole based on chitosan-coated iron oxide nanoparticles as a nanotherapy to fight Candida biofilms. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 192, AUG 2020. (17/24416-2)

CALDEIRAO, ANNE CAROLINE MORAIS; ARAUJO, HEITOR CEOLIN; TOMASELLA, CAMILA MIRANDA; SAMPAIO, CAIO; DOS SANTOS OLIVEIRA, MARCELO JOSE; RAMAGE, GORDON; PESSAN, JULIANO PELIM; MONTEIRO, DOUGLAS ROBERTO. Effects of Antifungal Carriers Based on Chitosan-Coated Iron Oxide Nanoparticles on Microcosm Biofilms. ANTIBIOTICS-BASEL, v. 10, n. 5, MAY 2021. (17/24416-2)