Genetic polymorphism associated to post-operative nausea and vomiting in patients submitted to cancer surgeries

• Grant number: 17/17914-6
• Support Opportunities: Regular Research Grants
• Duration: March 01, 2018 - October 31, 2020
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Angela Maria Sousa
• Grantee: Angela Maria Sousa
• Host Institution: Instituto do Câncer do Estado de São Paulo Octavio Frias de Oliveira (ICESP). Coordenadoria de Serviços de Saúde (CSS). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated researchers: Alexandre Slullitel ; Hazem Adel Ashmawi ; Miyuki Uno ; Roger Chammas ; Tatiane Katsue Furuya

Abstract

Nausea and vomiting affects 25-30% of patients after surgeries. In high-risk patients, this incidence can reach 70-80% of people. Identification of high-risk patients for PONV (through medical history and / or molecular testing) may restrict the number of potential candidates for a particular type of prophylactic anti-emetic therapy, indicating those most likely to benefit from the specific medication. Inherited factors may play a significant role in background sensitivity for PONV and CINV. Clinical research about hereditary factors involved in the pathogenesis of PONV and CINV is relatively new. The aim of this study is to investigate whether inter-individual differences related to PONV are associated with genetic factors. 300 patients will be evaluated in postoperative oncological surgeries and a blood sample (15mL) will be collected by peripheral vein puncture in tubes containing EDTA. This blood will later be processed (Buffy coat) and stored by the Biobank team. The DNA will be extracted from peripheral bood by the Salting Out Procedure and genotyped for 48 SNPs, from 15 candidate genes, using Real-time PCR by the Taqman method. The possible associations with demographic data and factors related to surgery will be analyzed by univariate and multivariate analysis. (AU)