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Effect of whey protein intake on heat shock proteins (HSPs) and intestinal microbiot in obese rats induced by cafeteria diet

Grant number: 17/16886-9
Support type:Regular Research Grants
Duration: March 01, 2018 - February 29, 2020
Field of knowledge:Health Sciences - Nutrition - Nutrition Biochemistry
Principal Investigator:Jaime Amaya Farfan
Grantee:Jaime Amaya Farfan
Home Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Assoc. researchers: Aline Rissetti Roquetto ; Andrey dos Santos ; Carolina Soares de Moura ; Mario Jose Abdalla Saad ; Pablo Christiano Barboza Lollo ; Priscila Neder Morato ; Valfredo de Almeida Santos Junior

Abstract

The whey protein (WP) have shown to have various properties and health effects. We recently reported that WP was able to increase expression of HSP70 in various tissues. The heat-shock proteins (HSP) are composed of several families that represent a protective system that favors cell resistance and tolerance in the face of adverse conditions in the body. It is believed that the high content of branched chain amino acids (BCAAs), is a major factor involved in the ability of WP increase in HSP70. The objective of this study is to investigate the influence of the consumption of whey protein as protein source in the diet on the expression of other HSPs, and also check the influence of amino acids and peptides present in the WP in HSPs and associated parameters. Methods: We will use the first experiment, 48 male rats were divided primarily into 2 groups: sedentary and exercised. Each group is divided into three dietary groups (receiving AIN 93-G, with protein replacement): whey protein concentrate (WPC), whey protein hydrolyzate (WPH) and casein (CAS) as a control. The animals of group exercised will be submitted to exercise on a treadmill as a source of heat stress. The following parameters are determined: free amino acids in the plasma, HSP25, HSP60, HSP90, AHA1, DHFR, NOS, VEGF, PI3-Kinase, BCKDH, SOD, Catalase, GPx, p-AKT, GFAT, OGT, HSF1, HSF1 phosphorylated. In Experiment 2, we use 90 male rats were divided into 10 groups, which are gavados one of the following amino acids: leucine, valine, isoleucine, glutamine and arginine, or peptides: isoleucyl-leucine, leucyl-isoleucine, valyl-leucine, leucyl-valine, compared to WPH. After the exercise as a source of stress, the following parameters are determined: free amino acids in plasma, HSP25, HSP60, HSP90, HSP70, AHA1, DHFR, NOS, VEGF, PI3-Kinase, BCKDH, SOD, catalase, GPx, p-AKT , GFAT, OGT, HSF1, HSF1 phosphorylated in skeletal muscle. The results will be analyzed by ANOVA and Tukey's test (using p d 0.05). (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ROQUETTO, ALINE RISSETTI; MOURA, CAROLINA SOARES; SANTOS-JUNIOR, VALFREDO DE ALMEIDA; SAMMARCO OLIVEIRA, PAULO OTAVIO; AIRES MACHADO, KARLA IDELCA; BORGES CASTRO CARVALHO, GESSIKA CRISTINA; RISSO, EDER MULLER; AMAYA-FARFAN, JAIME. Moderate intake of BCAA-rich protein improves glucose homeostasis in high-fat-fed mice. JOURNAL OF NUTRITIONAL BIOCHEMISTRY, v. 80, JUN 2020. Web of Science Citations: 0.
AIRES MACHADO, KARLA IDELCA; ROQUETTO, ALINE RISSETTI; MOURA, CAROLINA SOARES; LOPES, ALINE DE SOUZA; CRISTIANINI, MARCELO; AMAYA-FARFAN, JAIME. Comparative impact of thermal and high isostatic pressure inactivation of gram-negative microorganisms on the endotoxic potential of reconstituted powder milk. LWT-FOOD SCIENCE AND TECHNOLOGY, v. 106, p. 78-82, JUN 2019. Web of Science Citations: 0.
MOURA, CAROLINA SOARES; BARBOZA LOLLO, PABLO CHRISTIANO; MORATO, PRISCILA NEDER; AMAYA-FARFAN, JAIME. Dietary Nutrients and Bioactive Substances Modulate Heat Shock Protein (HSP) Expression: A Review. NUTRIENTS, v. 10, n. 6 JUN 2018. Web of Science Citations: 8.

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