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Investigation of the effect of cell sheet of human dental pulp stem cells associated or not with biophotonics (photobiomodulation therapy and antimicrobial photodynamic therapy) on murine tissue regeneration

Grant number: 17/16777-5
Support type:Regular Research Grants
Duration: March 01, 2018 - August 31, 2020
Field of knowledge:Health Sciences - Dentistry - Oral and Maxillofacial Surgery
Principal Investigator:Marcia Martins Marques
Grantee:Marcia Martins Marques
Home Institution: Universidade Ibirapuera (UNIB). Campus Chácara Flora. São Paulo , SP, Brazil
Assoc. researchers:Maria Cristina Zindel Deboni ; Maria Stella Nunes Araujo Moreira


The objective of this study will be to investigate the regeneration of bone lesions where the cell sheets (CSs) engineering will be applied associated with photobiomodulation therapy (PBMT) and antibacterial photodynamic therapy (aPDT) in rats presenting basic pathologies (Type 2 Diabetes mellitus) and in rats where the medication-related osteonecrosis of the jaws (MRONJ) will be induced by anti-resorptive drugs. The hypothesis of the study is that the association of cell sheet culture engineering with biophotonic therapies (PBMT and aPDT) will improve the regeneration of bone lesions in the mice bearing Type 2 Diabetes mellitus, as well as the regeneration of MRONJ lesions. The study will have 3 steps: one in vitro and the other two in vivo. In the first step, in vitro, CSs will be obtained to be applied in the treatment of bone lesions of the animals in the following in vivo steps. In vivo, two studies will be performed: first, MRONJ will be induced in 84 Wistar rats by using zolendronate, a potent antireabsorption medicatio, followed by upper molar extraction. Oral lesions will then be treated with application of CSs associated or not to PBMT and / or aPDT. In the second study, CSs, associated or not to PBMT, will be applied in 72 diabetic rats for treating standardized critical bone lesions in the parietal bone. The material obtained in both studies will be fixed and submitted to the analysis: computerized microtomography (MicroCT), histological (HE), histochemistry (TRAP- Tartrate Resistant Acid Phosphatase) and immunohistochemistry (cell tracking-BrDU and expression of markers involved in the osteogenesis process). The data will be statistically analyzed (pd0.05). (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FAGUNDES PEDRONI, ANA CLARA; SARRA, GIOVANNA; DE OLIVEIRA, NATACHA KALLINE; MOREIRA, MARIA STELLA; ZINDEL DEBONI, MARIA CRISTINA; MARQUES, MARCIA MARTINS. Cell sheets of human dental pulp stem cells for future application in bone replacement. CLINICAL ORAL INVESTIGATIONS, v. 23, n. 6, p. 2713-2721, JUN 2019. Web of Science Citations: 4.
PEDRONI, ANA C. F.; DINIZ, IVANA M. A.; ABE, GABRIELA L.; MOREIRA, MARIA S.; SIPERT, CARLA R.; MARQUES, MARCIA M. Photobiomodulation therapy and vitamin C on longevity of cell sheets of human dental pulp stem cells. Journal of Cellular Physiology, v. 233, n. 10, p. 7026-7035, OCT 2018. Web of Science Citations: 4.

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