Mitochondrial energy metabolism, inflammation and oxidative stress in obese women submitted to taurine supplementation and physical training

• Grant number: 17/10080-2
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2018
• End date: February 29, 2020
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Ellen Cristini de Freitas
• Grantee: Ellen Cristini de Freitas
• Host Institution: Escola de Educação Física e Esporte de Ribeirão Preto (EEFERP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Andrew Greenberg ; Camila Fernanda Costa e Cunha Moraes Brandão ; Flávia Giolo De Carvalho ; Julio Sergio Marchini
• Associated scholarship(s): 18/25095-8 - Effect of taurine supplementation associated with physical training on taurine and total amino acid concentrations in obese women, BP.TT

Abstract

Taurine supplementation researches has increased due to its antioxidant and anti-inflammatory actions, and its ability to modulate lipid metabolism by stimulating the expression of proteins that regulates mitochondrial biogenesis and increases respiratory function (PGC-1± and PPAR). Since obesity can induce metabolic disorders including abnormal production of adipokines and activation of pro-inflammatory signaling pathways also mitochondrial metabolism dysfunction in the adipose tissue, the use of taurine would be a new strategy for obesity prevention and treatment. Moreover, the association of taurine and exercise could improve exercise effects, promote higher energy expenditure and increase mitochondrial respiration, consequently resulting in weight loss. Methods: 45 obese women will be recruited for this study. The study will consist of 8-week supplementation period with taurine (3 grams) or placebo associated or not with exercise. In order to check the effects of the intervention, abdominal tissue biopsy will be performed for histological adipose tissue analysis, evaluation of mitochondrial function and quantification of uncoupling proteins (UPCs 1 and 2), PGC 1 ± and PPAR; blood collection will be done for quantification of taurine and of inflammatory markers (IL-10, IL-6, IL-1, TNF-± and CRP) and evaluation of telomeric length before and after the supplementation period. And body composition will be evaluated by deuterium oxide method. Outcomes: It is expected that taurine supplementation associated with exercise will increase mitochondrial function of the white adipose tissue, increase energy expenditure, decrease weight and body fat mass, and benefit the inflammatory profile of obese women. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE CARVALHO, FLAVIA G.; MUNOZ, VITOR R.; BRANDAO, CAMILA F. C.; SIMABUCO, FERNANDO M.; PAVAN, ISADORA C. B.; NAKANDAKARI, SUSANA C. B. R.; PAULI, JOSE RODRIGO; DE MOURA, LEANDRO P.; ROPELLE, EDUARDO R.; MARCHINI, JULIO SERGIO; et al. Taurine upregulates insulin signaling and mitochondrial metabolism in vitro but not in adipocytes of obese women. NUTRITION, v. 93, JAN 2022. (17/08036-5, 17/10080-2)

BATITUCCI, GABRIELA; CUNHA BRANDAO, CAMILA FERNANDA; DE CARVALHO, FLAVIA GIOLO; MARCHINI, JULIO SERGIO; PFRIMER, KARMA; FERRIOLI, EDUARDO; CUNHA, FERNANDO QUEIROZ; PAPOTI, MARCELO; BARROS MORHY TERRAZAS, SARA IVONE; JUNQUEIRA-FRANCO, MARCIA VARELLA MORANDI; et al. Taurine supplementation increases irisin levels after high intensity physical training in obese women. CYTOKINE, v. 123, NOV 2019. (17/08036-5, 17/10080-2)

DE CARVALHO, FLAVIA GIOLO; CUNHA BRANDAO, CAMILA FERNANDA; MUNOZ, VITOR ROSETTO; BATITUCCI, GABRIELA; DE ALMEIDA TAVARES, MARIA EDUARDA; TEIXEIRA, GIOVANA RAMPAZZO; PAULI, JOSE RODRIGO; DE MOURA, LEANDRO PEREIRA; ROPELLE, EDUARDO ROCHETE; CINTRA, DENNYS ESPER; et al. Taurine supplementation in conjunction with exercise modulated cytokines and improved subcutaneous white adipose tissue plasticity in obese women. Amino Acids, v. 53, n. 9, JUL 2021. (17/08036-5, 17/10080-2)

CUNHA BRANDAO, CAMILA FERNANDA; DE CARVALHO, FLAVIA GIOLO; SOUZA, ANDERSON DE OLIVEIRA; MORANDI JUNQUEIRA-FRANCO, MARCIA VARELLA; BATITUCCI, GABRIELA; COUTO-LIMA, CARLOS ANTONIO; FETT, CARLOS ALEXANDRE; PAPOTI, MARCELO; DE FREITAS, ELLEN CRISTINI; ALBERICI, LUCIANE CARLA; et al. Physical training, UCP1 expression, mitochondrial density, and coupling in adipose tissue from women with obesity. SCANDINAVIAN JOURNAL OF MEDICINE & SCIENCE IN SPORTS, v. 29, n. 11, JULY 2019. (17/10080-2)

CUNHA BRANDAO, CAMILA FERNANDA; NONINO, CARLA BARBOSA; DE CARVALHO, FLAVIA GIOLO; NICOLETTI, CAROLINA FERREIRA; NORONHA, NATALIA YUMI; SAN MARTIN, ROCIO; DE FREITAS, ELLEN CRISTINI; MORANDI JUNQUEIRA-FRANCO, MARCIA VARELLA; MARCHINI, JULIO SERGIO. The effects of short-term combined exercise training on telomere length in obese women: a prospective, interventional study. SPORTS MEDICINE-OPEN, v. 6, n. 1, JAN 16 2020. (17/10080-2)

DE CARVALHO, FLAVIA GIOLO; CUNHA BRANDAO, CAMILA FERNANDA; BATITUCCI, GABRIELA; SOUZA, ANDERSON DE OLIVEIRA; FERRARI, GUSTAVO DUARTE; ALBERICI, LUCIANE CARLA; MUNOZ, VITOR ROSETTO; PAULI, JOSE RODRIGO; DE MOURA, LEANDRO PEREIRA; ROPELLE, EDUARDO ROCHETE; et al. Taurine supplementation associated with exercise increases mitochondrial activity and fatty acid oxidation gene expression in the subcutaneous white adipose tissue of obese women. Clinical Nutrition, v. 40, n. 4, p. 2180-2187, APR 2021. (17/08036-5, 17/10080-2)

DE CARVALHO, MILENA BARBON; CUNHA BRANDAO, CAMILA FERNANDA; FASSINI, PRISCILA GIACOMO; BIANCO, THIAGO MANTELLO; BATITUCCI, GABRIELA; MARTINEZ GALAN, BRYAN STEVE; DE CARVALHO, FLAVIA GIOLO; VIEIRA, TALES SAMBRANO; FERRIOLLI, EDUARDO; MARCHINI, JULIO SERGIO; et al. Taurine Supplementation Increases Post-Exercise Lipid Oxidation at Moderate Intensity in Fasted Healthy Males. NUTRIENTS, v. 12, n. 5, MAY 2020. (17/10080-2, 17/08036-5)