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A wide genome association study for resistance to Fusarium oxysporum f. sp. phaseoli and for tegument color in common bean

Grant number: 17/24711-4
Support type:Regular Research Grants
Duration: March 01, 2018 - August 31, 2020
Field of knowledge:Agronomical Sciences - Agronomy
Principal Investigator:Luciana Lasry Benchimol-Reis
Grantee:Luciana Lasry Benchimol-Reis
Home Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil
Assoc. researchers:Alisson Fernando Chiorato ; Antonio Augusto Franco Garcia ; Luis Eduardo Aranha Camargo ; Renata Oliveira Batista
Associated scholarship(s):18/08582-2 - A wide genome association study for resistance to Fusarium oxysporum f. SP. phaseoli and for tegument color in common bean, BP.TT

Abstract

Fusarium wilt (Fop) stands out as being the main soil disease in bean crop, with a large occurrence worldwide, can cause losses of up to 100% in productivity in extreme cases. The most economical and efficient method for its control is the use of resistant cultivars, but the physiological variability of the pathogen makes difficult the development of resistant cultivars by breeding programs. Genome wide association studies (GWAS) allow the identification of loci that influence the expression of quantitative characteristics. In this context, the objective of this work will be to use a set of 6,000 SNPs (BARCBean6K_3 BeadChip) in 288 accessions of the IAC germplasm bank (BAG) to identify genomic regions associated with Fop resistance genes, grain staining, and browning of grains. Significant markers will be located, and chromosomes with the highest number of significant SNPs explain the highest proportion of variation in Fop resistance and tegument color, and indicate whether there are any genes and / or QTLs associated and in which regions of the genome. Accelerated grain darkening for 'carioca' type and Nuclear Magnetic Resonance analysis with all panel genotypes will be performed. For resistance to fusarium wilt, two isolates of Brazilian breed 2, characterized by UFV (Viçosa, MG) as virulent, will be used. Bulks containing 60 genotypes of clear tegument and 60 genotypes of dark tegument of the 'carioca' type will be analyzed with microsatellites in the search of polymorphic markers for selection assisted by markers. When polymorphic, these markers will be analyzed in all Bulk genotypes and will be incorporated into associative mapping. This study is expected to provide genetic evidence of the relationship between grain staining, browning and Fop resistance, providing tools to achieve lasting resistance to this disease. (AU)